Method for producing monounsaturated glycerides

A glyceride and unsaturated technology, applied in the field of glyceride, can solve the problems of increasing the risk of cardiovascular disease and raising blood cholesterol

Inactive Publication Date: 2011-05-04
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] A diet high in saturated fat is known to raise blood cholesterol and increase the risk of cardiovascular disease

Method used

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  • Method for producing monounsaturated glycerides
  • Method for producing monounsaturated glycerides
  • Method for producing monounsaturated glycerides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0084] The present invention is further described by the following examples, which should not be construed as limiting the scope of the present invention.

[0085] Example 1-Specificity Research of Candida Antarctica Lipase A

[0086] The enzyme Candida antarctica lipase A-Novozym 735-N735 (Lot: LDN00026) with 6KLU activity was used. Test substrates: Palm Stearine (PS); soybean oil (SBO); and high oleic oil, all purchased from Sigma.

[0087] A. Fat hydrolysis

[0088] 1. Weigh 200g of oil into an empty 500ml screw cap flask.

[0089] 2. Add 35g of water to the same flask and keep at oven temperature 70°C for one hour.

[0090] 3. Process by high shear mixing (IKA Ultra Turrax T25) at 24,000 rpm for 90 seconds.

[0091] 4. Transfer 100 g of the emulsion to an empty 250 ml screw cap flask.

[0092] 5. Add Novozym 735 at 600 ppm (51 μl). The dosage is based on the dry basis of the emulsion.

[0093] 6. Place the flask in a shaking water bath operating at 200 rpm and 70°...

Embodiment 2

[0136] Example 2 - Method of utilizing 1,3-specific lipolytic enzymes

[0137] In a method combining enzymatic reaction and separation, a glyceride product having an enriched content of monounsaturated fatty acids is produced.

[0138] 100 kg palm olein and 7.2 kg ethanol were used as raw materials for an enzymatic reaction using a 1,3-specific lipolytic enzyme (e.g. Lipozyme TL IM) to produce a mixture of monoglycerides, diglycerides, glycerol and fatty acid ethyl esters . The reaction was carried out at a temperature of 40°C. The molar ratio of palm olein to ethanol was 2:1, meaning that half a mole of ethanol was used for every mole of free fatty acid in palm olein. The reaction was carried out until almost all of the ethanol had reacted. The enzyme preferably reacts with the 1- and 3-positions. The reaction product contains ethyl esters mainly from saturated fatty acids.

[0139] The mixture is separated by distillation or evaporation into two fractions: (1) an ethyl ...

Embodiment 3

[0146] Example 3 - Method of Utilizing a Lipolytic Enzyme Selective for Saturated Fatty Acids

[0147] In a method combining enzymatic reaction and separation, a glyceride product having an enriched content of monounsaturated fatty acids is produced.

[0148] 100 kg of palm olein and 7.2 kg of ethanol were used as raw materials for an enzymatic reaction using a lipase (e.g. Candida antarctica lipase A) preferably reacting with saturated fatty acids to produce monoglycerides, diglycerides, glycerol and fatty acid B mixture of esters. The reaction was carried out at a temperature of 40°C. The molar ratio of palm olein to ethanol was 2:1, meaning that half a mole of ethanol was used for every mole of free fatty acid in palm olein. The reaction was carried out until almost all of the ethanol had reacted. The reaction products include ethyl esters mainly from saturated fatty acids.

[0149] The mixture is separated by distillation or evaporation into two fractions: (1) an ethyl...

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Abstract

A process for producing a glyceride product which is enriched in monounsaturated fatty acids relative to the starting glyceride comprising the steps: (a) alcoholysis of triglycerides employing lipolytic enzymes selective for saturated fatty acids and / or lipolytic enzymes selective for the 1-position, the 3-position or both positions in a glyceride; and (b) separation of fraction A which is enriched in saturated fatty acid esters from fraction B which is enriched in monounsaturated glycerides.

Description

technical field [0001] The present invention relates to the technical field of glycerides. It involves the manufacture of glycerides by using lipolytic enzymes. More specifically, the present invention relates to methods for producing monounsaturated glycerides by using specific lipolytic enzymes. Background technique [0002] A diet high in saturated fat is known to raise blood cholesterol and increase the risk of cardiovascular disease. Therefore, it is desirable to reduce the amount of saturated fat and increase the amount of unsaturated fat in consumer products. [0003] Several reports have been published disclosing methods for producing polyunsaturated fats. WO 95 / 24459 (Norsk Hydro A / S) describes a process applied to fish oils in which a fraction rich in polyunsaturated glycerides is separated from a fraction with saturated fatty acids and monounsaturated glycerides. Instead of recombining the fractions to form triglycerides, they continued alcoholysis until essen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6454C12P7/649Y02E50/10C12P7/6458
Inventor 珀·M·尼尔森斯蒂芬·厄恩斯特金·博尔奇汉斯·C·霍尔姆伊·H·森威廉·D·考恩
Owner NOVOZYMES AS
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