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Feed supplement

A supplement, feed technology, applied in the field of feed including feed supplements, which can solve problems such as limited growth or feed efficiency

Inactive Publication Date: 2012-07-11
DUPONT NUTRITION BIOSCIENCES APS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This fact limits its utilization in animal feed and possible improvement of growth or feed efficiency in animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0439] Preparation of phosphate standard curve (absolute method)

[0440] use KH 2 PO 4 Buffer (1.2) A standard curve was prepared. Standards were diluted with acetate buffer.

[0441] C 稀释 = 0.0mM, 0.5mM, 1.0mM, 1.5mM, 2.0mM, 2.5mM, 3.0mM, 4.0mM

[0442] Use the following formula: C 原液 *V 原液 =C 稀释 *V 稀释

[0443] V 原液 =(C 稀释 *V 稀释 ) / C 原液

[0444] where C = concentration, V = volume

[0445]

[0446]1.00 ml of the standard curve sample was mixed with 2.00 ml of staining / stopping reagent and then mixed with 2.00 ml of phytate solution. Samples were incubated at room temperature for 5-10 minutes and OD was measured (measured on samples after incubation).

[0447] sample preparation :

[0448] 1A: Liquid product (approximately 5500FTU / g): Weigh 1g of sample into a 100ml volumetric flask, record the weight, and fill the volumetric flask with acetate buffer.

[0449] 1B: Dry product (phytase formulated on wheat / starch carrier only): 1 g of prod...

Embodiment

[0501] Example 1 - Introduction

[0502] In order to be effective as an enzyme additive in food or animal feed, phytase must have a variety of different properties. To be able to degrade phytic acid in the acidic environment of the animal's stomach, it must be active at low pH, preferably over a broad pH range. In addition, it must have a high specific activity and preferably a high thermostability so that it, as a protein, can withstand the high temperatures common in the preparation of feed, such as pelleted feed.

[0503] Also importantly, the enzyme has a broad substrate specificity that allows it to hydrolyze not only phytate but also intermediates of phytate degradation such as inositol pentaphosphate, tetraphosphate and triphosphate. Studies on the degradation of phytate in pigs have shown that without this enzyme these inositol oligophosphates remain largely insoluble in the small and large intestines, leaving them inaccessible to alkaline phosphatase produced by th...

Embodiment 2

[0508] Embodiment 2 pepsin stability

[0509] Phytase variants BP-17, BP-110, BP-111, BP-112 and Phyzyme XP from Buttjuia sp. compared to Natuphos (BASF) and Ronozyme P (Novozymes / DSM) at pH 2 Pepsin resistance.

[0510] Materials and methods

[0511] buffer :

[0512] Pepsin incubation buffer: 0.1 M Glycine-HCl, pH 2.0, 3 mg / ml BSA, 2.9 mg anhydrous sodium chloride / mL, 0.73 mg calcium chloride / mL. For pepsin-containing solutions, incubation buffers were prepared to contain 500, 1000, 3000, 6000 or 10000 U / ml pepsin (SigmaP-7000, 10000 U / mg corresponds to 27 mg / ml), respectively. One pepsin unit is defined as will produce a ΔOD of 0.001 per minute at pH 2.0, 37°C 280 The amount of enzyme was measured as a soluble product of TCA using hemoglobin as substrate (American Food Chemicals Codex).

[0513] Phytase assay buffer: Acetate buffer 250mM, pH 5.5

[0514] Phytase assay buffer containing BSA: Acetate buffer 250mM, pH 5.5, containing 3mg / ml BSA

[0515] Resistance...

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Abstract

The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.

Description

technical field [0001] The present invention relates to feed supplements. More specifically, the present invention relates to feed supplements comprising phytases and lipolytic enzymes useful for improving the digestion of animal feed and to feedstuffs comprising the feed supplements. Background technique [0002] Phytate is the main storage form of phosphorus in cereals and legumes. However, monogastric animals such as pigs, poultry and fish are not able to metabolize or absorb phytate (or phytic acid), so it is excreted, leading to phosphorus pollution in areas where livestock is intensively kept. In addition, phytic acid also acts as an antinutrient in monogastric animals by chelating metals such as calcium, copper and zinc. [0003] In order to provide sufficient phosphate for the growth and health of these animals, inorganic phosphate is added to their diet. Such additions can be costly and further exacerbate pollution problems. [0004] Phytate is usually hydrolyze...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165C12N9/16C12N9/20
CPCA23K1/1653A23K1/184C12N9/20A23K1/1826A61K38/465C12N9/16A23L1/30A23K20/189A23K50/75A23K50/30A23L33/10A61P1/00A61P3/02A23K20/147A23K20/168A23K20/195A23K20/26A23K20/00
Inventor M·F·伊萨克森R·H·洛伦特森P·普卢姆斯泰德L·F·罗梅罗米兰S·M·马德里C·林M·沃德M·R·扎尔加希
Owner DUPONT NUTRITION BIOSCIENCES APS
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