Cell seedling temperature cryopreservation method and device

A cell and cryopreservation technology, which is applied to the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of not being able to effectively determine the success or failure of ice planting and the effect, so as to improve the effect of cryopreservation and avoid excessive ice planting or planting. The effect of lack of ice
CN113207872APending Publication Date: 2021-08-06UNIV OF SHANGHAI FOR SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
UNIV OF SHANGHAI FOR SCI & TECH
Publication Date
2021-08-06

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Abstract

The invention discloses a cell seedling temperature cryopreservation method and a device, and belongs to the technical field of cell cryopreservation, the method comprises the following steps: S1, cooling a cryopreservation tube loaded with cells to be cryopreserved through a refrigeration system according to a preset cooling rate, and detecting the temperature of the cryopreservation tube in the cooling process; S2, judging whether the temperature of the cryopreservation tube reaches a preset seedling temperature or not, if yes, executing S3, and if not, executing S1 continuesly; S3, adopting the refrigeration system to conduct heat preservation on the cryopreservation tube at the preset seedling temperature; S4, applying ultrasonic waves to the cryopreservation tube through an ultrasonic vibrator so as to carry out seedling temperature operation, and lasting the operation for preset threshold time; S5, judging whether seedling temperature operation of the cryopreservation tube succeeds or not by analyzing the temperature of the cryopreservation tube before and after the seedling temperature operation, if yes, executing S6, and if not, continuing to execute S4; and S6, cooling the cryopreservation tube continuesly through the refrigerating system, stopping cooling after the cryopreservation tube is cooled to the preset cryopreservation temperature, and carrying out heat preservation. According to the method, the cell cryopreservation effect is improved by adding the step of judging whether seedling temperature operation succeeds or not.
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Description

technical field

[0001] The invention relates to the technical field of cell cryopreservation, in particular to a cell plantation cryopreservation method and device. Background technique

[0002] Low temperature preservation is a routine practice for laboratories and sample banks to preserve cells and tissues, but cells and tissues are often damaged during the cooling process. The main factors that cause cell and tissue damage are the toxic damage of cryoprotectants and the intracellular ice damage caused by the formation of intracellular ice during freezing.

[0003] Among them, the reason for intracellular ice damage is that during the process of programmed freezing, when the solution temperature is lower than the eutectic temperature but still does not crystallize, the cell solution will be supercooled, and when the supercooling degree is large, it will suddenly nucleate, and then cause Cells are damaged by large ice crystals.

[0004] One of the keys to solving intracel...

Claims

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