Freezing preservation solution used for adipose-derived stem cells as well as preparation method for freezing preservation solution and culture medium

A culture medium and amino acid technology, applied in the field of cryopreservation solution, can solve the problems of lack of serum-free medium research, etc., and achieve the effect of enhancing cryopreservation effect, promoting protein expression, enhancing cryopreservation effect and preservation effect
CN106561632AInactive Publication Date: 2017-04-19浙江译美生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
浙江译美生物科技有限公司
Publication Date
2017-04-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a freezing preservation solution used for adipose-derived stem cells as well as a preparation method for the freezing preservation solution and a culture medium. The culture medium comprises the following components in parts by weight: 7200-7300 parts of inorganic salt composite, 980-1000 parts of amino acid complex, 40-45 parts of vitamin composite, 1000-1100 parts of glucose, 100-110 parts of sodium pyruvate, 60-75 parts of butyric acid, 80-110 parts of sodium butyrate, 0.15-0.22 part of ferric citrate and 1,000,000 parts of water. The freezing preservation solution has the beneficial effects that: the culture medium has excellent freezing preservation effect in the freezing preservation solution which is compounded by a serum substitute component KSR and dimethyl sulfoxide, and is used for the adipose-derived stem cells.
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Description

technical field

[0001] The invention relates to the field of cryopreservation liquid, in particular to a cryopreservation liquid for adipose stem cells, a preparation method thereof and a culture medium. Background technique

[0002] Bone marrow stem cells, umbilical cord stem cells and adipose-derived stem cells (ADSCs) are several types of stem cells that have been studied more in recent years. Bone marrow stem cells are difficult to obtain, slow to expand, unsuitable for frail and elderly patients, and ethical issues, which bring inconvenience to clinical application. Compared with bone marrow stem cells, umbilical cord mesenchymal stem cells have a wide range of sources, low immunogenicity, and almost no immune rejection. Adipose stem cells are a kind of stem cells with multi-directional differentiation potential isolated from adipose tissue in recent years. Compared with bone marrow stem cells and umbilical cord stem cells, ADSCs have many advantages. Studies have foun...

Claims

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