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Cell cryopreservation solution, method for cryopreserving hematopoietic stem cells by using cell cryopreservation solution and stem cell preparation

A stem cell preparation and hematopoietic stem cell technology, which is applied in the field of cryopreservation solution, can solve the problems of easy aggregation of cells and low cell viability, and achieve the effects of simple composition, high cell viability and high viability.

Inactive Publication Date: 2021-03-12
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a cell cryopreservation solution and a method for freezing and storing hematopoietic stem cells, which solves the problem that the hematopoietic stem cells stored in the traditional hematopoietic stem cell freezing solution are low in cell viability and easy to form agglomerates after resuscitation

Method used

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  • Cell cryopreservation solution, method for cryopreserving hematopoietic stem cells by using cell cryopreservation solution and stem cell preparation
  • Cell cryopreservation solution, method for cryopreserving hematopoietic stem cells by using cell cryopreservation solution and stem cell preparation
  • Cell cryopreservation solution, method for cryopreserving hematopoietic stem cells by using cell cryopreservation solution and stem cell preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of cell freezing solution

[0033] For every 10ml of cell cryopreservation solution: add 4ml of Bomaili A injection, 5ml of 20wt% human albumin injection, and 1ml of clinical-grade DMSO into a centrifuge tube, and gently blow and mix. Prepare cryopreservation solution in the amount of 3-5 tubes per 10-15ml of cord blood volume, and pre-cool in a 4°C refrigerator.

Embodiment 2

[0035] Preparation of cell freezing solution

[0036] The difference between this example and Example 1 is that each 10mL cryopreservation solution contains 5ml of Bomaili A injection, 4ml of human serum albumin, and 1ml of clinical grade DMSO.

Embodiment 3

[0038] Preparation of cell freezing solution

[0039] The difference between this example and Example 1 is that each 10mL cryopreservation solution contains 2ml Bomaili A injection, 6ml human albumin, and 2ml clinical grade DMSO.

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Abstract

The invention relates to the technical field of cryopreservation solutions, in particular to a cell cryopreservation solution, a method for cryopreserving hematopoietic stem cells by using the cell cryopreservation solution and a stem cell preparation. The invention discloses a cell cryopreservation solution which is composed of DMSO, a human serum albumin injection and a plasmon A injection. Thecell cryopreservation liquid is simple and safe in components, the components are matched with one another, a synergistic effect is achieved, the cell cryopreservation effect can be remarkably improved, the survival rate of cryopreserved hematopoietic stem cells after recovery is high, the cell stability is good, and treatment of diseases is facilitated.

Description

technical field [0001] The invention relates to the technical field of cryopreservation liquid, in particular to a cell cryopreservation liquid, a method for freezing and storing hematopoietic stem cells, and a stem cell preparation. Background technique [0002] The cell cryopreservation solution can prevent cells from being damaged during the freezing process. The traditional hematopoietic stem cell cryopreservation solution uses 10% DMSO + 90% fetal bovine serum to freeze hematopoietic stem cells. The non-apoptotic cells are surrounded by substances to form flocs and even the cells are clustered and the cells are unstable. After the clustered cells are imported into the human body, it is easy to cause fever in the patient. Therefore, it is very important to effectively control the viability and stability of hematopoietic stem cells after cryopreservation. Contents of the invention [0003] The invention provides a cell cryopreservation solution and a method for freezin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 陈海佳王小燕杨祖婷张梦晨
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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