Macrophage cryopreservation liquid and macrophage cryopreservation method

A macrophage and cryopreservation method technology, applied in the field of cell cryopreservation, can solve the problems of unsuitability for clinical use, cumbersome operation steps, and few passages, and achieve the effects of improving cryopreservation effect, long passage time, and reducing content.
CN105900973AActive Publication Date: 2016-08-31GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Publication Date
2016-08-31

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Abstract

The invention relates to macrophage cryopreservation liquid and a macrophage cryopreservation method. The macrophage cryopreservation liquid is prepared from the following components: glucan, bovine serum albumin, sorbitol, low molecular dextran, chitosan and RPMI 1640. The macrophage cryopreservation method utilizes the macrophage cryopreservation liquid to cryopreserve macrophages. According to the macrophage cryopreservation liquid and the macrophage cryopreservation method, the cryopreservation effect of the macrophages is improved, the survival rate is relatively high after recovery, the growth state of cells is good and the generation time is relatively long; the macrophage cryopreservation liquid does not utilize FBS (Fasting Blood Sugar) so that the safety of clinical transfusion is greatly improved; and the content of DMSO (Dimethylsulfoxide) is reduced and the toxin risks to human bodies are reduced.
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Description

technical field

[0001] The invention relates to the field of cell cryopreservation, in particular to a macrophage cryopreservation solution and a cryopreservation method. Background technique

[0002] Macrophages are white blood cells located in tissues and belong to phagocytic cells, which participate in non-specific defense (innate immunity) and specific defense (cellular immunity) in vertebrates. Their main function is to phagocytize (i.e. phagocytize and digest) cell debris and pathogens in the form of fixed or free cells, and activate lymphocytes or other immune cells to respond to pathogens. Therefore, macrophages also have a certain ability to present antigens.

[0003] At present, there are few studies on macrophage cryopreservation. In fact, macrophages are extremely sensitive to cryopreservation conditions due to their long digestion time and terminal differentiation. The existing cryopreservation solution for frozen macrophages consists of 90v / v% FBS and 10v / v%...

Claims

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