Hepatocyte ultrasonic planting and freezing storage device and freezing storage method thereof

Abstract

The invention relates to an ultrasonic plant freezing and storing device, in particular to an ultrasonic plant freezing and storing device for hepatocytes and a freezing and storing method of the ultrasonic plant freezing and storing device. The ultrasonic plant-freezing storage device comprises an ultrasonic container, a temperature detection system, an ultrasonic induced nucleation system and a cooling system, the liver cell ultrasonic plant-freezing preservation method comprises the following steps: culturing liver cells, determining an upper threshold of a plant-freezing temperature, selecting a temperature-controlled phase-change liquid raw material, and carrying out liver cell ultrasonic plant-freezing preservation. Compared with a conventional hepatocyte cryopreservation mode, the ultrasonic plant-freezing cryopreservation device and the ultrasonic plant-freezing cryopreservation method have the advantages that the hepatocyte cryopreservation effect is remarkably improved, the use amount of the cryoprotectant is small, the ultrasonic plant ice and the cryopreservation protectant have a synergistic effect, the recovery survival rate of the cryopreserved hepatocytes is higher than 90%, and no significant difference exists between the cryopreserved hepatocytes and fresh groups; and an unexpected technical effect is achieved.

Description

technical field

[0001] The present invention relates to an ultrasonic implant freezing device, in particular to an ultrasonic implant freezing device for liver cells and a freezing method thereof. Background technique

[0002] Cell cryopreservation is a key technology used in modern regenerative medicine, organ transplantation and assisted reproduction. Cell therapy is currently recognized as an effective means of fighting cancer and other intractable diseases, and has attracted widespread attention from all parties. The cryopreservation of cells is generally done by freezing the cells to a lower subzero temperature (usually -196°C). The cells are at low temperatures and have no vital activities, so they can be stored for a long time. However, severe cell damage may occur during the cooling process. damage. In order to reduce freezing damage, the commonly used cell freezing scheme is to add a certain concentration of cryoprotectant to the cell solution, and then freeze by s...

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