Method for extracting phenolic glycoside compounds from semen lepidii and application thereof

The invention relates to a technique for extracting and extracting the bellflower seeds, which is applied in the field of extracting phenolic glycoside compounds to achieve the effects of improving cell survival rate and protecting cardiomyocytes.

Active Publication Date: 2017-07-07
HENAN UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
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Problems solved by technology

However, there have been no relevant reports on the isolation of new compounds from the water extract of Tinglizi and the study of their applications.

Method used

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  • Method for extracting phenolic glycoside compounds from semen lepidii and application thereof
  • Method for extracting phenolic glycoside compounds from semen lepidii and application thereof
  • Method for extracting phenolic glycoside compounds from semen lepidii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 8 kg of Tinglizi, stir-fry for 5.5 minutes at 240°C, add water 10 times the weight of Tinglizi to extract three times, each time for 1.5h, concentrate the extract under reduced pressure to extract, and use 20L volume for the concentrated extract The concentration is 80% ethanol precipitation, the supernatant is centrifuged, concentrated to no alcohol smell, put on Dianion HP-20 column (adsorption / desorption resin of highly porous styrene), successively water, 20% ethanol (v / v), 40% ethanol (v / v), 60% ethanol (v / v), and 95% ethanol (v / v) were eluted, and each part was concentrated and dried under reduced pressure to obtain water, 20% ethanol, 40 % ethanol, 60% ethanol, 95% ethanol elution (respectively 361.0g, 71.2g, 89.6g, 67.2g, 28.7g) components, wherein 20% ethanol elution components pass through Toyopearl HW-40 column chromatography, Gradient elution with water, 10% methanol, 20% methanol, 30% methanol, and 100% methanol in sequence to obtain components A1-A5....

Embodiment 2

[0036]Take 2 kg of Tinglizi, stir-fry for 5.5 minutes at 240°C, add water 10 times the weight of Tinglizi to extract three times, each time for 1.5h, concentrate the extract under reduced pressure to extract, and use a volume of 5L for the concentrated extract The concentration is 80% ethanol precipitation, the supernatant is centrifuged, concentrated to no alcohol smell, put on Dianion HP-20 column (adsorption / desorption resin of highly porous styrene), successively water, 20% ethanol (v / v), 40% ethanol (v / v), 60% ethanol (v / v), and 95% ethanol (v / v) were eluted, and each part was concentrated and dried under reduced pressure to obtain water, 20% ethanol, 40 % ethanol, 60% ethanol, 95% ethanol elution (respectively 90.1g, 18.0g, 22.5g, 17.0g, 7.3g) components, wherein 20% ethanol elution components pass through Toyopearl HW-40 column chromatography, Gradient elution with water, 10% methanol, 20% methanol, 30% methanol, and 100% methanol in sequence to obtain components A1-A5...

Embodiment 3

[0038] Take 1 kg of Tinglizi, stir-fry at 240°C for 5.5 minutes, add water 10 times the weight of Tinglizi to extract three times, each time for 1.5 hours, concentrate the extract under reduced pressure to the extract, and use 3L volume of the concentrated extract The concentration is 80% ethanol precipitation, the supernatant is centrifuged, concentrated to no alcohol smell, put on Dianion HP-20 column (adsorption / desorption resin of highly porous styrene), successively water, 20% ethanol (v / v), 40% ethanol (v / v), 60% ethanol (v / v), and 95% ethanol (v / v) were eluted, and each part was concentrated and dried under reduced pressure to obtain water, 20% ethanol, 40 % ethanol, 60% ethanol, 95% ethanol elution (respectively 45.0g, 9.0g, 11.3g, 8.5g, 3.6g) fractions, of which 20% ethanol elution fraction (9.0g) passed through Toyopearl HW- 40 column chromatography, followed by gradient elution with water, 10% methanol, 20% methanol, 30% methanol, and 100% methanol, to obtain compo...

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Abstract

The invention relates to a method for extracting phenolic glycoside compounds from semen lepidii and application thereof, which can effectively obtain the new function of the semen lepidii. The method adopts the technical scheme that the molecular formula of a semen lepidii new glycoside A is C18H24O12[M+Na]<+>m/z 455.1196; the molecular formula of a semen lepidii new glycoside B is C18H24O12[M+Na]<+>m/z 455.1202. The method has the advantages that two new phenolic glycoside compounds are extracted from a water extract of the semen lepidii, namely the semen lepidii new glycoside A and the semen lepidii new glycoside B; by adopting the two new phenolic glycoside compounds, the cell survival rate of the injured hydrogen peroxide-induced rat myocardial cell H9c2 is obviously improved; the function of protecting the myocardial cell is realized, and a new path is provided for preparation of myocardial protection drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for extracting phenolic glycosides from Tinglizi chinensis and its application. Background technique [0002] Tinglizi is a commonly used Chinese herbal medicine. It was first recorded in "Shen Nong's Materia Medica", and it was listed as a herbal inferior product. The medicine tastes pungent and bitter, and is very cold in nature. The "Tinglizi" included in the 2015 edition of "Chinese Pharmacopoeia" has a north-south distinction. Among them, the dry mature seeds of the cruciferous plant Lepidium apetalum Willd. .) The dry mature seeds of Webbex Prantl. are commonly called "Nan Ting Li Zi". Modern pharmacology shows that Tinglizi mainly has cardiotonic effect, and its chemical composition is mainly flavonoids. However, there are no related reports on the isolation of new compounds from the water extract of Tinglizi and the study of their applications. Contents of the inventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08A61K31/7034A61P9/00
CPCC07H1/08C07H15/203
Inventor 郑晓珂李孟杨方方冯卫生张志广张靖柯吕锦锦赵璇
Owner HENAN UNIV OF CHINESE MEDICINE
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