Serum-free protein-free cell culture medium

A medium, protein-free technology, applied in cell culture medium, culture process, tissue culture, etc., can solve the problem of low recombinant protein expression, achieve excellent results, reduce pollution risks, and reduce production costs.

Active Publication Date: 2017-05-10
昆明润什生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country has also developed some serum-free and protein-free media, but these media can only support cell growth at low cell densities, and the expression of recombinant proteins is also relatively low. Protein medium is an important topic in the field of medium research

Method used

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  • Serum-free protein-free cell culture medium
  • Serum-free protein-free cell culture medium
  • Serum-free protein-free cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Serum-free and protein-free cell culture medium with defined components, including amino acids, vitamins, inorganic salts and trace elements, carbohydrates and other chemicals, polyamines or their derivatives, and its specific component content is composed of:

[0037] The composition of amino acids and their content per liter of medium are as follows:

[0038]

[0039]

[0040] The composition of vitamins and their content per liter of medium are as follows:

[0041]

[0042]

[0043] The composition of inorganic salts and trace elements and their content per liter of medium are as follows:

[0044]

[0045] Other trace elements and their contents per liter of medium are as follows:

[0046] ZnCl2 1–100 μM

[0047] or ZnSO4-7H2O 1–100 μM

[0048] or Zn(NO3)2-6H2O 1–100 μM

[0049] Other trace elements and their contents per liter of medium are as follows:

[0050] Ferric Ammonium Citrate 1–500mg

[0051] or ferric citrate 1–500 μM

[0052] or EDTA...

Embodiment 2

[0058] Serum-free and protein-free cell culture medium with defined components, including amino acids, vitamins, inorganic salts and trace elements, carbohydrates and other chemicals, polyamines or their derivatives, and its specific component content is composed of:

[0059] The composition and content of amino acids:

[0060]

[0061]

[0062] Composition and content of vitamins:

[0063]

[0064]

[0065] The composition and content of inorganic salts:

[0066]

[0067] Composition and content of trace elements:

[0068] CuSO4-7H2O 124.84mg / L

[0069] MnCl2-4H2O 98.955mg / L

[0070] Na2SeO3 17.294mg / L

[0071] Composition and content of other trace elements:

[0072] ZnCl2 68.15mg / L

[0073] Or ZnSO4-7H2O 143.78mg / L

[0074] Or Zn(NO3)2-6H2O 148.745mg / L

[0075] Composition and content of other trace elements:

[0076] Ferric Ammonium Citrate 2g / L

[0077] Or ferric citrate 489.88mg / L

[0078] Or sodium iron edetate 734.1mg / L

[0079] Composition and...

Embodiment 3

[0132] Serum-free and protein-free cell culture medium with defined components, including amino acids, vitamins, inorganic salts and trace elements, carbohydrates and other chemicals, polyamines or their derivatives, and its specific component content is composed of:

[0133] The composition and content of amino acids:

[0134]

[0135]

[0136] Composition and content of vitamins:

[0137]

[0138] The composition and content of inorganic salts:

[0139]

[0140]

[0141] Composition and content of trace elements:

[0142] CuSO4-7H2O 124.84mg / L

[0143] MnCl2-4H2O 98.955mg / L

[0144] Na2SeO3 17.294mg / L

[0145] Composition and content of other trace elements:

[0146] ZnCl2 68.15mg / L

[0147] Composition and content of other trace elements:

[0148] Ferric Ammonium Citrate 2g / L

[0149] Or ferric citrate 489.88mg / L

[0150] Or sodium iron edetate 734.1mg / L

[0151] Composition and content of carbohydrates and other chemicals:

[0152]

[0153] Com...

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PUM

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Abstract

The invention relates to the technical field of biology, specifically discloses a serum-free protein-free cell culture medium, and aims to provide a serum-free protein-free cell culture medium capable of supporting the growth of high density cells. The culture medium comprises amino acids, vitamins, inorganic salts, trace elements, carbohydrates, other chemical compounds, polyamine (or derivatives of polyamine), and hydrolysate. The manufacturing cost is low. The stability is high, the live cell density and cell survival rate are extremely high, and the culture medium is suitable for culture cells for recombinant protein and vaccine production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a serum-free and protein-free cell culture medium. Background technique [0002] Animal cells have been widely used in the production of recombinant protein drugs and vaccines, and culture medium is the key technology for large-scale cultivation of animal cells. To culture cells in traditional medium, 5-10% serum should be added to the traditional medium. Serum is expensive, easily infected by viruses and mycoplasma, and the quality between batches is unstable. Serum contains a large amount of protein, which brings great difficulties to the separation and purification of recombinant protein cell products. In order to overcome the above problems, scientists have found that insulin, transferrin and other growth factors in serum are the main components to promote cell growth, and developed insulin, transferrin and other growth factors in serum-free medium. Because these growth factor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07
CPCC12N5/0037C12N5/0043C12N5/005C12N2500/05C12N2500/22C12N2500/24C12N2500/30C12N2500/32C12N2500/38C12N2500/46C12N2500/92C12N2500/95
Inventor 陈少军陈少敏
Owner 昆明润什生物科技有限公司
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