Serum-free protein-free cell culture medium
A medium, protein-free technology, applied in cell culture medium, culture process, tissue culture, etc., can solve the problem of low recombinant protein expression, achieve excellent results, reduce pollution risks, and reduce production costs.
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Embodiment 1
[0036] Serum-free and protein-free cell culture medium with defined components, including amino acids, vitamins, inorganic salts and trace elements, carbohydrates and other chemicals, polyamines or their derivatives, and its specific component content is composed of:
[0037] The composition of amino acids and their content per liter of medium are as follows:
[0038]
[0039]
[0040] The composition of vitamins and their content per liter of medium are as follows:
[0041]
[0042]
[0043] The composition of inorganic salts and trace elements and their content per liter of medium are as follows:
[0044]
[0045] Other trace elements and their contents per liter of medium are as follows:
[0046] ZnCl2 1–100 μM
[0047] or ZnSO4-7H2O 1–100 μM
[0048] or Zn(NO3)2-6H2O 1–100 μM
[0049] Other trace elements and their contents per liter of medium are as follows:
[0050] Ferric Ammonium Citrate 1–500mg
[0051] or ferric citrate 1–500 μM
[0052] or EDTA...
Embodiment 2
[0058] Serum-free and protein-free cell culture medium with defined components, including amino acids, vitamins, inorganic salts and trace elements, carbohydrates and other chemicals, polyamines or their derivatives, and its specific component content is composed of:
[0059] The composition and content of amino acids:
[0060]
[0061]
[0062] Composition and content of vitamins:
[0063]
[0064]
[0065] The composition and content of inorganic salts:
[0066]
[0067] Composition and content of trace elements:
[0068] CuSO4-7H2O 124.84mg / L
[0069] MnCl2-4H2O 98.955mg / L
[0070] Na2SeO3 17.294mg / L
[0071] Composition and content of other trace elements:
[0072] ZnCl2 68.15mg / L
[0073] Or ZnSO4-7H2O 143.78mg / L
[0074] Or Zn(NO3)2-6H2O 148.745mg / L
[0075] Composition and content of other trace elements:
[0076] Ferric Ammonium Citrate 2g / L
[0077] Or ferric citrate 489.88mg / L
[0078] Or sodium iron edetate 734.1mg / L
[0079] Composition and...
Embodiment 3
[0132] Serum-free and protein-free cell culture medium with defined components, including amino acids, vitamins, inorganic salts and trace elements, carbohydrates and other chemicals, polyamines or their derivatives, and its specific component content is composed of:
[0133] The composition and content of amino acids:
[0134]
[0135]
[0136] Composition and content of vitamins:
[0137]
[0138] The composition and content of inorganic salts:
[0139]
[0140]
[0141] Composition and content of trace elements:
[0142] CuSO4-7H2O 124.84mg / L
[0143] MnCl2-4H2O 98.955mg / L
[0144] Na2SeO3 17.294mg / L
[0145] Composition and content of other trace elements:
[0146] ZnCl2 68.15mg / L
[0147] Composition and content of other trace elements:
[0148] Ferric Ammonium Citrate 2g / L
[0149] Or ferric citrate 489.88mg / L
[0150] Or sodium iron edetate 734.1mg / L
[0151] Composition and content of carbohydrates and other chemicals:
[0152]
[0153] Com...
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