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Cell-gel material composite microsphere as well as preparation method and application thereof

A gel material and composite microsphere technology, applied in the field of biomedical materials, can solve the problems of loading cells, poor mechanical properties, material breakage, etc., and achieve the effects of maintaining the biological properties of collagen, centralized distribution, and simple preparation method.

Active Publication Date: 2015-03-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In these researches and applications, in addition to exhibiting good biological properties, collagen-based biomaterials also exhibit defects such as poor mechanical properties leading to material fragmentation and poor permeability resulting in slow mass transfer. Although the combined treatment can improve its mechanical properties, the modification process is a great threat to the loaded cells.

Method used

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  • Cell-gel material composite microsphere as well as preparation method and application thereof
  • Cell-gel material composite microsphere as well as preparation method and application thereof
  • Cell-gel material composite microsphere as well as preparation method and application thereof

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preparation example Construction

[0049] The preparation process flow of the present invention is (as Figure 9 shown): the pH of the gel material solution is adjusted with the cell suspension to make a gel material-cell mixture, which is dispersed in an oily solution and gelled, and then the obtained product is washed to obtain a cell-loaded mixture. Microspheres; microspheres are then dispersed in the continuous phase solution of temperature-sensitive materials or photosensitive materials, and gelled to form heterogeneous hydrogels.

[0050] Among them, when temperature-sensitive materials are used, the cell material mixture needs to be mixed under low temperature conditions; when photosensitive materials are used, a photoinitiator needs to be added to the cell material mixture; dispersion treatment is to make the cell material mixture Disperse in oily solution; after continuous stirring and dispersion for a period of time, gelatinize the crude microspheres; obtain microspheres after repeated washing and sep...

Embodiment 1

[0053] (1) Take 200mL rapeseed oil, add a small amount of Tween-60, stir the rapeseed oil with a magnetic stirrer at a speed of 1500rpm in an ice bath, and slowly add 1mL of a collagen solution with a concentration of 15mg / mL to the dish Stir continuously in the seed oil for 20 minutes, raise the temperature of the above water-oil phase mixture to 37°C, and keep stirring for 10 minutes. After the stirring is completed, clean and separate the mixture, remove the oil phase, and obtain an average particle size of about 30 micron collagen microspheres.

[0054] (2) Disperse the collagen microspheres prepared in step (1) in a collagen solution with a concentration of 15 mg / mL, transfer the above-mentioned collagen solution containing microspheres to a mold, raise the temperature to 37°C and keep for 30 minutes to obtain collagen Heterogeneous Collagen Hydrogel with Collagen Microspheres Encapsulated by Continuous Phase. The resulting hydrogel was dissected, and under a scanning el...

Embodiment 2

[0056] (1) Take 4mL of olive oil and 6mL of corn oil and stir and mix at a speed of 800rpm to obtain a mixed oil phase solution. Under stirring conditions, 4mL of hyaluronic acid (transparent Hyaluronic acid) solution (the concentration of hyaluronic acid is 3 mg / mL) was slowly added dropwise into the above mixed oil phase solution, and stirred continuously for 30 min. The wavelength is 365nm, the light intensity is 30mW / cm 2 The ultraviolet radiation of 360s triggers the polymerization reaction, gels the hyaluronic acid microspheres, and keeps stirring for 10 minutes; washes and separates the mixed solution, removes the oil phase, and obtains hyaluronic acid microspheres with an average particle size of about 1000 microns. ball.

[0057] (2) The hyaluronic acid microspheres prepared in step (1) are dispersed in the hyaluronic acid solution modified by maleic anhydride with a concentration of 8 mg / mL, and the above-mentioned hyaluronic acid solution containing microspheres is...

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Abstract

The invention discloses a cell-gel material composite microsphere as well as a preparation method and an application thereof and belongs to the field of biomedical materials. The cell-gel material composite microsphere is prepared from a gel microsphere and cells distributed in the gel microsphere. The preparation method comprises the following steps: dispersing a gel material-cell mixed solution in an oily solution, and carrying out emulsification and gelation treatment to obtain the cell-gel material composite microsphere. The cell-gel material composite microsphere is controllable in cell loading ratio, high in cell survival rate, small in volume shrinkage, difficult in breaking, good in transmitting capability of nutrients and metabolites, simple in preparation method and convenient in popularization and application and can also be used for preparing nonhomogeneous hydrogel so as to obtain a material with high biomimetic degree.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a cell-gel material composite microsphere and its preparation method and application. technical background [0002] Biomedical gel materials (such as collagen, etc.) can create an environment close to the extracellular matrix, provide space for cell adhesion, growth and proliferation, and have good biocompatibility and biodegradability. Biological activity and function, so it has become an important material in the research and application of biomedical materials, and has shown high value in the fields of scientific research and clinical application. [0003] In the existing reports, the vast majority of collagen hydrogels are in the size range of millimeters to centimeters, and are studied or applied in bulk form. In these researches and applications, in addition to good biological properties, collagen-based biomaterials also have defects such as poor mechanical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/24A61L27/20A61L27/18A61L27/52C12N5/077C12N5/0775C12N5/071
Inventor 林海刘钧樊渝江张兴栋
Owner SICHUAN UNIV
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