High-precision ultrasonic anti-blocking multi-cell biological additive manufacturing method and device

A kind of additive manufacturing and multi-cell technology, applied in additive processing, medical science, prosthesis, etc., can solve the problems of inaccurate cell positioning, low printing efficiency, nozzle clogging, etc., to improve survival rate, facilitate clamping, The effect of reducing the probability of congestion

Active Publication Date: 2017-05-31
JILIN UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a high-precision ultrasonic anti-blocking multi-cell biological additive manufacturing method and device to sol

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  • High-precision ultrasonic anti-blocking multi-cell biological additive manufacturing method and device
  • High-precision ultrasonic anti-blocking multi-cell biological additive manufacturing method and device
  • High-precision ultrasonic anti-blocking multi-cell biological additive manufacturing method and device

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Embodiment Construction

[0037] Include the following steps:

[0038] 1) Before printing, put the printing device into the disinfection cabinet or sterilization box for 15 minutes, and finally put it into the sterile test room or the sterile test box to wait for printing;

[0039] 2) Inject the biological solution containing various cells into the storage chamber connected to the nozzle one, and apply a strength of 20mw / cm to the nozzle 2 , ultrasonic frequency 200KHz, open the nozzle-piezoelectric drive device and the storage chamber-pneumatic device, the piezoelectric drive device and the ultrasonic wave form a composite driving force, and the droplet ejection efficiency is higher;

[0040] 3) The high-strength composite hydrogel is injected into the second storage chamber connected to the special nozzle for printing blood vessels, and the pneumatic device of the second storage chamber is turned on, waiting for printing;

[0041] 4) Place a printed polyethylene (PE) organ base with 80% porosity on ...

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Abstract

The invention relates to a high-precision ultrasonic anti-blocking multi-cell biological additive manufacturing method and device, and belongs to the technical field of biological additive manufacturing. A three-axis moving platform is mounted under a horizontal moving shaft, a multi-nozzle component is mounted on the horizontal moving shaft, multiple materials and cells are sprayed by piezoelectric driving and ultrasonic-assisted technology, hydrogel is transformed into sol outside the cells by a micron-sized nozzle, an ultrasonic sprayer device and an ultrasonic anti-blocking method, the cells are accurately positioned and deposited by the micron-sized nozzle, and complicated structural organs are accurately printed in a multi-material, multi-cell and high-activity manner. Reduction of printing accuracy due to enlargement of the nozzle diameter of a traditional cell printer for preventing blockage of a sprayer is avoided, limitation of multi-cell and multi-material composite printing and single-cell accurate printing is overcome, the problems of low cell survival rate and the like in the multi-cell and multi-material printing process are solved, and three-dimensional forming and integrated printing of complicated biological organ tissues are realized.

Description

technical field [0001] The invention relates to the technical field of biological additive manufacturing, in particular to a method and device for three-dimensional organ additive manufacturing of multi-material and multi-cell additive manufacturing. Background technique [0002] Organ printing is a bioprinting method that uses the principle of additive manufacturing to manufacture organs using bio-inks made from stem cell differentiation as raw materials. Organs are composed of intricate vascular networks, tissues, muscles, nerves, etc., requiring printers to have the ability to print multi-material and multi-cells; organs themselves have compact tissues and a large number of capillaries, resulting in very small spacing between cells, requiring printers It can deposit cells with high precision and print biological materials accurately; in the process of cell printing, the nozzle diameter of the nozzle is generally reduced to meet the precise positioning of the cells, and th...

Claims

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Application Information

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IPC IPC(8): A61F2/02B33Y30/00B33Y10/00
CPCA61F2/02A61F2240/001B33Y10/00B33Y30/00
Inventor 吴文征叶稳立刘巍耿鹏李桂伟赵继任露泉
Owner JILIN UNIV
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