40results about How to "Use of technique" patented technology

Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.

The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.

An orthodontic bracket or convertible buccal tube for use with arch wires has the usual mesial distal extending slot having one side open to receive the wire. The open side is closed by a shutter pivoting on a pivot pin, or coaxial pins, about a mesial distal axis, the shutter being latched in slot closed position to retain the arch wire by a latch having one latch member on the pivot pin / s and a cooperating member on an annular part of the shutter surrounding the pin / s. Preferably the latch members are a mesial distal extending cam shaped recess in the pivot pin / s and a projection protruding from the annular shutter part, or vice versa. The part of the annular shutter part carrying the latch member is made flexible by a mesial distal extending through slot dividing it to form a movable segment of about 200° to 270° of the total circumference; a tool can be inserted into the slot to assist in disengaging the latch. The shutter member can have parts thereof that with the shutter in slot closed position are an interference fit against the device body providing an additional retaining force against opening movement. Preferably the device includes an attitude controlling spring member consisting of a thin sheet metal spring within the body having a free end extending into the arch wire slot to engage an arch wire therein, or alternatively consisting of a flexible extension of the part of the shutter member that closes the slot.

An orthodontic bracket or convertible buccal tube for use with arch wires has the usual mesial distal extending slot having one side open to receive the wire. The open side is closed by a shutter pivoting on a pivot pin, or coaxial pins, about a mesial distal axis, the shutter being latched in slot closed position to retain the arch wire by a latch having one latch member on the pivot pin / s and a cooperating member on an annular part of the shutter surrounding the pin / s. Preferably the latch members are a mesial distal extending cam shaped recess in the pivot pin / s and a projection protruding from the annular shutter part, or vice versa. The part of the annular shutter part carrying the latch member is made flexible by a mesial distal extending through slot dividing it to form a movable segment of about 200° to 270° of the total circumference; a tool can be inserted into the slot to assist in disengaging the latch. The shutter member can have parts thereof that with the shutter in slot closed position are an interference fit against the device body providing an additional retaining force against opening movement. Preferably the device includes an attitude controlling spring member consisting of a thin sheet metal spring within the body having a free end extending into the arch wire slot to engage an arch wire therein, or alternatively consisting of a flexible extension of the part of the shutter member that closes the slot.

Proposed is a system and method for forecasting frequencies associated to future loss and loss distributions for individual risks of a plurality of operating units (30) with at least one measurable liability exposure (31) by means of independently operated liability risk drivers (311-313), and for related automated operation of a loss resolving unit (40) by means of a control unit controller (10). In case of an occurring loss at a loss unit (20, . . . , 26) measure parameters are measured and transmitted to the control unit controller (10). The control unit controller (10) dynamically assigned the measure parameters to the liability risk drivers (311-313) and tunes the operation of the loss resolving unit (40) by resolving the occurred loss by means of the loss resolving unit (40).

The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications.

An isolation circuitry and method are provided for coupling between a power supply and processing circuitry in order to provide power to the processing circuitry whilst hiding a power consumption characteristic of that processing circuitry. The isolation circuitry comprises a plurality of sub-circuits, with each sub-circuit comprising a capacitor, a first switch configured to provide a first connection between the capacitor and the power supply, a second switch configured to provide a second connection between the capacitor and the processing circuitry, and a third switch configured to provide a third connection across the capacitor to partially discharge the capacitor. Control circuitry controls the plurality of sub-circuits, such that within each sub-circuit the first switch, second switch and third switch are placed in an active state in a repeating sequence. Each of the plurality of sub-circuits further comprises a comparator configured to place the third switch in an open state when a predetermined non-zero voltage difference across the capacitor is reached during the active state of the third switch. By such an approach, it is ensured that the voltage across the comparator at the end of the discharge operation is always the same irrespective of the voltage present at the start of the discharge operation. As a result, the power consumption characteristic of the processing circuitry is entirely hidden by the isolation circuitry. Further, the isolation circuitry of the present invention provides a particular power efficient mechanism for hiding the power consumption characteristic of the processing circuitry.

A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 mul of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 mul volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99° C., 1 second-66° C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent. According to the present invention, the procedures in the genetic engineering studies and industries involved with PCR can be speeded up.

Described are amphiphilic polymers that are provided with chelating moieties. The amphiphilic polymers are block copolymers comprising a hydrophilic block and a hydrophobic block, with the chelating moieties linked to the end-group of the hydrophilic block. The disclosed polymers are capable of self-assembly into structures such as micelles and polymersomes. With suitable metals present in the form of coordination complexes with 5 the chelating moieties, the chelating amphiphilic polymers of the invention are suitable for use in various imaging techniques requiring metal labeling, such as MRI (T 1 / T 2 weighted contrast agents or CEST contrast agents) SPECT, PET or Spectral CT.

Where code execution results in an error attributable to multiple data elements, the code is revised so the error results from one element. Where execution improperly functions without error, and the elements contain required but missing properties, the code is improperly functioning in its required-property handling. Errors are organized into classes and error-causing elements are organized into independent sets corresponding to the classes. Elements that are not within any set are determined. Symptom, error, no-error, refined-symptom, and function databases can be employed during code execution analysis. Symptom database entries correspond to elements and indicate errors, or that no errors, are attributable to the elements. Error database entries correspond to elements resulting in errors upon execution. No-error database entries correspond to elements resulting in no errors upon execution. Refined-symptom database entries correspond to root cause elements of errors. Function database entries correspond to root cause elements of no errors.

A networked system of computers, connected dynamically, often very temporarily (e.g. phone call, text, web search), based on individual criteria unique to and controlling each, as they temporarily opt in to the system to evaluate information. All may process data obtained from a database mashed together by combining highly persistent (years) with highly transitory (minutes to weeks) data, temporarily bound thereto. Distributed processing by individual, independent processors is controlled by their own individual criteria, facilitating population, storing, indexing, searching, navigation, querying, and serving of heretofore unwieldy content. Applications execute heretofore impossible procedures as dynamic as situational awareness and personal decisions by their operators. In one example, transitory events can be incorporated by combining persistent data records with temporary ones on a customized site serving an event calendar from a media site, and commercial entities can tap into predictions based thereon.

A package for a semiconductor chip or other heat producing device has a supporting substrate to which the devices mount and electrically connect. An enclosure is formed over the heat producing devices and filled with a supercritical fluid that transports heat from the devices to a heat sink in thermal contact with the enclosure.

Methods for applying injectable fillers are provided. In some embodiments, the methods can result in a reduced risk of an adverse event occurring from the administration of the injectable filler. Also disclosed are devices and kits related to this method.

System and method for measuring an optical property of a sub micrometer region of a sample including interacting a probe tip of a probe microscope with a region of the sample, illuminating the sample with a beam of light from a radiation source such that light is scattered from the probe-sample interaction region, interfering a reference beam with the scattered light wherein the reference beam has an adjustable optical phase, measuring with a detector at least a portion of the light scattered from probe-sample and background regions at a substantially constant reference phase, and constructing a signal indicative of the optical property of the sample wherein contributions from background scattered light are substantially suppressed.

A method for segmenting a digitized medical image including providing a digitized image comprising a plurality of intensities corresponding to a domain of points on a n-dimensional grid, modeling the segmentation phases of said image with 2k weighting functions defined as a product of k unknown auxiliary functions, minimizing an energy functional of said weighting functions and said auxiliary functions to derive k gradient flow equations for said auxiliary functions, numerically integrating said gradient flow equations wherein solutions for the k unknown auxiliary functions are obtained, and partitioning said image into segments based on a membership of each image point in each of said weighting functions.

A risk parsing device provides risk splitting and loss event diversification for a multi-risk forecast systems capturing multiple loss aggregation under complex environmental conditions based on the occurrence of defined loss events. The loss events are triggered by one or more involved causing units and affected units, wherein the loss events evolve from one or multiple risk accumulating sources. Risk events related loss measures can be scattered over multiple causing units or multiple related clash events. The multi-risk forecast system provides loss-generating processes and events, wherein the inventive risk splitter is a technical core element to generate an event-related loss distribution mirroring cause-effect chains induced by the risk events which extend over space and time. The risk splitter module allows automated splitting of the risk based on risk exposed, causing unit characteristics and risk exposed affected units' characteristics. The causing unit characteristics comprises for each causing unit assigned activity characteristic parameters and participation characteristic parameters of said causing unit defining for a specific causing unit a specific set of executable activity link members and market share driving participation probabilities. The causing unit characteristics comprises quota parameters defining affected populations of affected units with incidence and defining refined effect types.

An orthodontic bracket or convertible buccal tube for use with arch wires has the usual mesial distal extending slot having one side open to receive the wire. The open side is closed by a shutter pivoting on a pivot pin, or coaxial pins, about a mesial distal axis, the shutter being latched in slot closed position to retain the arch wire by a latch having one latch member on the pivot pin / s and a cooperating member on an annular part of the shutter surrounding the pin / s. Preferably the latch members are a mesial distal extending cam shaped recess in the pivot pin / s and a projection protruding from the annular shutter part, or vice versa. The part of the annular shutter part carrying the latch member is made flexible by a mesial distal extending through slot dividing it to form a movable segment of about 200° to 270° of the total circumference; a tool can be inserted into the slot to assist in disengaging the latch. The shutter member can have parts thereof that with the shutter in slot closed position are an interference fit against the device body providing an additional retaining force against opening movement. Preferably the device includes an attitude controlling spring member consisting of a thin sheet metal spring within the body having a free end extending into the arch wire slot to engage an arch wire therein, or alternatively consisting of a flexible extension of the part of the shutter member that closes the slot.

In a method and apparatus for acquiring cardiac image data of a subject of examination, in particular in the context of magnetic resonance tomography, a first image acquiring technique is implemented, in which image data acquiring takes place only in a part of the cardiac cycle, and / or not in each cardiac cycle, and a second image acquiring technique is alternatingly implemented, such that the image data acquiring using the second image acquiring technique takes place at times in which image data acquiring using the first image acquiring technique is not occurring.

Described are amphiphilic polymers that are provided with chelating moieties. The amphiphilic polymers are block copolymers comprising a hydrophilic block and a hydrophobic block, with the chelating moieties linked to the end-group of the hydrophilic block. The disclosed polymers are capable of self-assembly into structures such as micelles and polymersomes. With suitable metals present in the form of coordination complexes with 5 the chelating moieties, the chelating amphiphilic polymers of the invention are suitable for use in various imaging techniques requiring metal labeling, such as MRI (T 1 / T 2 weighted contrast agents or CEST contrast agents) SPECT, PET or Spectral CT.

This disclosure describes examples of a filter media for use in filter devices, e.g., pulse filter cartridges found in power generating systems. Embodiments of the filter media include a base media and a membrane, which partially covers one or both sides of the base media. In one embodiment, the membrane covers a membrane area of at least one side of the base media, wherein the membrane area is less than a total area of the side on which the membrane is disposed.

A method of the detection of particle contamination on a semiconductor wafer is provides which includes examining an area of the semiconductor wafer by a metrology system comprising a scatterometry or ellipsometry / reflectometry tool to obtain measured metrology data, comparing the measured metrology data with reference metrology data and determining the presence of particle contamination in the examined area of the semiconductor wafer based on the comparison of the measured metrology data with the reference metrology data.

A device and method for enterally hydrating a patient with cooled fluids. The patient may be a paralyzed or limited upper body mobility patient with a functional gastrointestinal system who is nonetheless incapable or not fully capable of drinking unassisted. Here fluids are provided by a suspended container which also has a pouch to admit cooling material such as ice, but to keep the cooling material separate from the fluid. The container may also have optional graduation markings to allow for convenient estimation of fluid use, and an upper open and closeable cap to allow the container to be refilled. The container has a lower fluid conducting tube that terminates in a mouthpiece. The mouthpiece contains a fluid valve formed from at least one slit in an elastic material. Patient mouth force on the valve causes the slit to enlarge, thus enabling cooled fluid to flow into the patient.

A device and method for enterally hydrating a patient with cooled fluids. The patient may be a paralyzed or limited upper body mobility patient with a functional gastrointestinal system who is nonetheless incapable or not fully capable of drinking unassisted. Here fluids are provided by a suspended container which also has a pouch to admit cooling material such as ice, but to keep the cooling material separate from the fluid. The container may also have optional graduation markings to allow for convenient estimation of fluid use, and an upper open and closeable cap to allow the container to be refilled. The container has a lower fluid conducting tube that terminates in a mouthpiece. The mouthpiece contains a fluid valve formed from at least one slit in an elastic material. Patient mouth force on the valve causes the slit to enlarge, thus enabling cooled fluid to flow into the patient.

A package for a semiconductor chip or other heat producing device has a supporting substrate to which the devices mount and electrically connect. An enclosure is formed over the heat producing devices and filled with a working fluid including a chemical compound that reacts endothermically to absorb heat produced by the devices and releases the heat in a reverse reaction to the enclosure.

A magnet system for use in a nuclear magnetic resonance (“NMR”) apparatus includes a first magnet and a second magnet located on a backplane to form a gap therebetween, wherein the first magnet and the second magnet are each shaped to form trapezoidal prisms with dimensions selected to optimize a magnetic field at a target region in space external to the magnet system.