Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gene of sheep type brucella M5 mycopremna ribosomal protein L7, its encoding protein and application

A Brucella and ribosomal protein technology, applied in the field of genetic engineering, can solve the problems of susceptibility to Brucella melis, strong toxic reaction, and health hazards in animal husbandry, etc.

Inactive Publication Date: 2008-04-02
INNER MONGOLIA UNIVERSITY
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there are many deficiencies in the existing vaccines, and the crowd is the most susceptible to Brucella melis and has the strongest toxic reaction, the infection rate of Brucellosis has been rising in a straight line in recent years, which has a great impact on the development of animal husbandry and human beings. serious harm to the health of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene of sheep type brucella M5 mycopremna ribosomal protein L7, its encoding protein and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The following examples are used to further illustrate the present invention, but are not intended to limit the scope of the present invention.

[0014] 1. Culture of Brucella melis M5 strain

[0015] First resuscitate Brucella melis M5 strain freeze-dried bacteria (purchased from China Institute for the Control of Pharmaceutical and Biological Products) with Brucella broth medium, and Brucella broth medium (purchased from China Import and Export Commodity Inspection Technology Research Institute) solid Streak culture on a slant at 37°C, 5% CO 2 Cultivate under the same conditions for 10 days, after Brucella grows on the solid slope to form a single colony visible to the naked eye, carefully scrape a single colony with an inoculation loop, inoculate it into liquid Brucella broth medium, and culture it in a water bath shaker at 37°C After 24 hours, a bacterial solution with a suitable concentration was obtained.

[0016] 2. Extraction of Bacterial Whole Genomic DNA

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a gene of ribosomal protein L7 / L12 of strain M5 of brucellaovis as well as the encoding protein and the application of the gene. The encoding protein of the gene of the present invention has the amino acid sequence of SEQ ID NO.2. Gene vaccine can be prepared by utilizing the gene of the present invention; siRNA or shRNA or microRNA, which is designed and synthesized according to the sequence of the present invention, can be used for gene silencing. Developing a recombinant protein vaccine or exploring a detection kit can be realized by preparing the expressing protein of the gene of the present invention, thereby providing new effective solving ways to brucellosis.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to the gene of ribosomal protein L7 / L12 of Brucella melitensis M5 strain, its coded protein and application. Background technique [0002] Brucellosis is a zoonotic infectious disease caused by Brucella. Brucella is a mammalian parasite that is highly infectious and pathogenic to humans and mammals. The genus has seven biological species: sheep, cattle, pigs, dogs, wood rats, marine mammals and sheep. The first four species are mainly popular in China. Livestock such as sheep, cattle, and pigs are most susceptible to infection, and sheep are the most common. Because Brucella is a facultative intracellular parasite, it not only parasitizes the mononuclear-macrophage system, but also reproduces in placental villous epithelial cells, renal parenchymal cells, testicular fibroblasts, and embryonic cells, causing reproductive Inflammation of organs and membranes, abortion, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/23C12N15/63A61K31/7088A61K39/10C12Q1/68G01N33/68
Inventor 石艳春郑源强旭日干
Owner INNER MONGOLIA UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com