Gene of sheep type brucella M5 mycopremna ribosomal protein L7, its encoding protein and application

A Brucella and ribosomal protein technology, applied in the field of genetic engineering, can solve the problems of susceptibility to Brucella melis, strong toxic reaction, and health hazards in animal husbandry, etc.

Inactive Publication Date: 2008-04-02
INNER MONGOLIA UNIVERSITY
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Problems solved by technology

Because there are many deficiencies in the existing vaccines, and the crowd is the most susceptible to Brucella melis and has the strongest toxic reaction, the infection rate of Brucellosis has been rising in a straight line in recent years, which has a great impact on the development of animal husbandry and human beings. serious harm to the health of

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  • Gene of sheep type brucella M5 mycopremna ribosomal protein L7, its encoding protein and application

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Embodiment Construction

[0013] The following examples are used to further illustrate the present invention, but are not intended to limit the scope of the present invention.

[0014] 1. Culture of Brucella melis M5 strain

[0015] First resuscitate Brucella melis M5 strain freeze-dried bacteria (purchased from China Institute for the Control of Pharmaceutical and Biological Products) with Brucella broth medium, and Brucella broth medium (purchased from China Import and Export Commodity Inspection Technology Research Institute) solid Streak culture on a slant at 37°C, 5% CO 2 Cultivate under the same conditions for 10 days, after Brucella grows on the solid slope to form a single colony visible to the naked eye, carefully scrape a single colony with an inoculation loop, inoculate it into liquid Brucella broth medium, and culture it in a water bath shaker at 37°C After 24 hours, a bacterial solution with a suitable concentration was obtained.

[0016] 2. Extraction of Bacterial Whole Genomic DNA

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Abstract

The present invention provides a gene of ribosomal protein L7/L12 of strain M5 of brucellaovis as well as the encoding protein and the application of the gene. The encoding protein of the gene of the present invention has the amino acid sequence of SEQ ID NO.2. Gene vaccine can be prepared by utilizing the gene of the present invention; siRNA or shRNA or microRNA, which is designed and synthesized according to the sequence of the present invention, can be used for gene silencing. Developing a recombinant protein vaccine or exploring a detection kit can be realized by preparing the expressing protein of the gene of the present invention, thereby providing new effective solving ways to brucellosis.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to the gene of ribosomal protein L7 / L12 of Brucella melitensis M5 strain, its coded protein and application. Background technique [0002] Brucellosis is a zoonotic infectious disease caused by Brucella. Brucella is a mammalian parasite that is highly infectious and pathogenic to humans and mammals. The genus has seven biological species: sheep, cattle, pigs, dogs, wood rats, marine mammals and sheep. The first four species are mainly popular in China. Livestock such as sheep, cattle, and pigs are most susceptible to infection, and sheep are the most common. Because Brucella is a facultative intracellular parasite, it not only parasitizes the mononuclear-macrophage system, but also reproduces in placental villous epithelial cells, renal parenchymal cells, testicular fibroblasts, and embryonic cells, causing reproductive Inflammation of organs and membranes, abortion, ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/23C12N15/63A61K31/7088A61K39/10C12Q1/68G01N33/68
Inventor 石艳春郑源强旭日干
Owner INNER MONGOLIA UNIVERSITY
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