Drug for inhibiting proliferation of tumor cells and application thereof

Abstract

The invention discloses a polypeptide for inhibiting proliferation of tumor cells, which is of a fragment with maximum length of 145 amino acid residues at 46-100 position from N-terminal, comprising ribosomal protein RPL26. The reporter gene experiments, Western blotting, co-immunoprecipitation and cell proliferation detection experiments confirm that RPL26 as well as MDM2 and p53 form a complex, and the combination of RPL26 and MDM2 can obviously reduce the ubiquitin ligase activity of MDM2, thereby improving the stability and transcriptional activation of p53. The overexpression of RPL26 can obviously inhibit the proliferation of the tumor cells, and the function is related to the activity of p53. RPL26 can be assisted for the clinical treatment of cancer and has great significance.

Description

technical field

[0001] The invention relates to a medicine for inhibiting tumor cell proliferation and its application. Background technique

[0002] In normal mammalian cells, the tumor suppressor protein p53 is mainly maintained at a low level by ubiquitin ligase E3 and 26S proteasomal degradation pathway. When cells face stress signals such as oncogene activation and DNA damage, the degradation of the tumor suppressor protein p53 will be inhibited, the amount of p53 will increase rapidly, the transcriptional activation function will be activated, and the activation of downstream target genes will be induced, thereby causing cell cycle arrest. Stress responses such as stagnation, apoptosis, or DNA damage repair. Studies have shown that the change of p53 protein level has a great relationship with its function. Therefore, the p53 ubiquitination degradation pathway is very important for the regulation of p53 function.

[0003] MDM2 is an important ubiquitin ligase E3 resp...

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