Multiple stain reagent and detection method for identifying breast myoepithelial lesion

A detection method and multiple staining technology, applied in the preparation of test samples, etc., can solve the problems of time-consuming and labor-intensive methods, long staining time, cumbersome methods, etc., to improve clarity, reduce repeated puncture biopsies, and overcome discontinuity and jumping effects

Inactive Publication Date: 2013-07-10
王刚平
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AI Technical Summary

Problems solved by technology

If multiple tissue antigens are displayed on one slice at the same time, it is often necessary to use multiple labeling on one slice to repeatedly mark, and five kinds of antibodies need to be stained five tim

Method used

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Embodiment Construction

[0030] A multiple staining immunohistochemical reagent for identifying breast myoepithelial lesions, the immunohistochemical reagent consists of the following antibodies by weight: mouse anti-human CK8 / 18 immunohistochemical monoclonal antibody 20%, ready-to-use calcium regulation Protein mouse anti-human monoclonal antibody 20%, ready-to-use cytokeratin mouse anti-human monoclonal antibody 20%, ready-to-use P63 protein mouse anti-human monoclonal antibody 20%, actin HHF-35 mouse anti-human monoclonal Antibody 20%, the production method is to draw equal amounts of the above-mentioned antibodies of each component, then mix them, rotate them on a vortex mixer or blow them with a pipette to mix them, and store them in a 4°C refrigerator for later use.

[0031] Before carrying out multiple staining, the pre-experiments of individual staining were firstly performed, and the experimental conditions were the same as those for multiple staining.

[0032] The multi-staining reagent imm...

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Abstract

The invention discloses a multiple stain reagent and a detection method for identifying a breast myoepithelial lesion. The multiple stain reagent comprises 20% of mouse anti-human CK8/18 immunohistochemical monoclonal antibody, 20% of ready-to-use calmodulin mouse anti-human monoclonal antibody, 20% of ready-to-use cytokeratin mouse anti-human monoclonal antibody, 20% of ready-to-use P63 protein mouse anti-human monoclonal antibody, and 20% of actin HHF-35 anti-human monoclonal antibody. When a breast myoepithelial cell is marked, the myoepithelial cell has strong specificity and sensibility, and different target antigens in the same target cell can be identified, so that a cell nucleus, a cell membrane and a cytoplasm can be stained synchronously, the reactivity and the sensibility of the myoepithelial cell are markedly improved, basic structures of a breast acinus and a catheter can be indicated more objectively, and a good assistance effect is exerted on the diagnosis of the breast lesion.

Description

technical field [0001] The invention relates to an immunohistochemical reagent, in particular to a multiple staining reagent for distinguishing breast myoepithelial lesions and a detection method thereof. Background technique [0002] Breast tissue often contains a variety of antigenic components, which are expressed in different parts of the cell membrane, cytoplasm and nucleus of different types of cells. In pathological diagnosis of breast diseases, different antigens of myoepithelial tissue and glandular epithelial tissue often need to be stained and marked with various antibodies on multiple sections. If the myoepithelial tissue exists continuously, there is no invasive cancer, and if the myoepithelial tissue is negative, it means breast invasion. Carcinoma (except blind duct adenosis). A single antibody showed that myoepithelial cells of ductal carcinoma in situ were jumping positive, and myoepithelial discontinuity. If multiple tissue antigens are displayed on one s...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
Inventor 王刚平
Owner 王刚平
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