Multiple stain reagent and detection method for identifying breast myoepithelial lesion

Abstract

The invention discloses a multiple stain reagent and a detection method for identifying a breast myoepithelial lesion. The multiple stain reagent comprises 20% of mouse anti-human CK8 / 18 immunohistochemical monoclonal antibody, 20% of ready-to-use calmodulin mouse anti-human monoclonal antibody, 20% of ready-to-use cytokeratin mouse anti-human monoclonal antibody, 20% of ready-to-use P63 protein mouse anti-human monoclonal antibody, and 20% of actin HHF-35 anti-human monoclonal antibody. When a breast myoepithelial cell is marked, the myoepithelial cell has strong specificity and sensibility, and different target antigens in the same target cell can be identified, so that a cell nucleus, a cell membrane and a cytoplasm can be stained synchronously, the reactivity and the sensibility of the myoepithelial cell are markedly improved, basic structures of a breast acinus and a catheter can be indicated more objectively, and a good assistance effect is exerted on the diagnosis of the breast lesion.

Description

technical field [0001] The invention relates to an immunohistochemical reagent, in particular to a multiple staining reagent for distinguishing breast myoepithelial lesions and a detection method thereof. Background technique [0002] Breast tissue often contains a variety of antigenic components, which are expressed in different parts of the cell membrane, cytoplasm and nucleus of different types of cells. In pathological diagnosis of breast diseases, different antigens of myoepithelial tissue and glandular epithelial tissue often need to be stained and marked with various antibodies on multiple sections. If the myoepithelial tissue exists continuously, there is no invasive cancer, and if the myoepithelial tissue is negative, it means breast invasion. Carcinoma (except blind duct adenosis). A single antibody showed that myoepithelial cells of ductal carcinoma in situ were jumping positive, and myoepithelial discontinuity. If multiple tissue antigens are displayed on one s...

AI Technical Summary

Problems solved by technology

If multiple tissue antigens are displayed on one slice at the same time, it is often necessary to use multiple labeling on one slice to repeatedly mark, and five kinds of antibodies need to be stained five times. The method is cumbersome, the staining time is long, and repeated on one slice. The operation is easy to cause tissue detachment, and it is time-consuming and laborious from a methodological point of view