41 results about "Aristolochic acid" patented technology

This invention publics a detecting method about a nontoxic traditional medicine liao asarum. It is to extract volatile oil according to a method of volatile oil extraction from Chinese pharmacopeia, the 2000 edition, dissolve by cyclohexane, and inject it into gas-chromatograph-mass spectrometer to draft fingerprint pattern. Through the analysis result by computer, it is to get 28-wave crest, such as methyleugenol, safrole myristicin and so on, where the essential component methyleugenol is about 37 percent. The volatile oil extracted according to pharmacopeia is dissolved by acetic ester, added methyl alcohol, and injected into high efficiency liquid chromatography to get high efficiency liquid fingerprint pattern. It is to adopt liquid hypersonic extracted for 30 minutes by methyl alcohol-water (3:1) ,and inject it into high efficiency liquid chromatography to build detecting method about high efficiency liquid chromatogram for aristolochic acid.

The invention discloses a quality detection method for a Tibetan medicine composition rheumatism Theron preparation. With the adoption of the quality detection method, the quality standard of an existing rheumatism Theron preparation is correspondingly improved and an identification method for shellac and elecampane in a previous standard is optimized; the identification on juniperus formosana, syzygium aromaticum, meconopsis, rubia wallichiana decne and amomum kravanh is increased on the basis of the previous standard, and a content determination method of general flavone and swertia chirayita and a detection method for aristolochic acid A are increased, so as to further ensure the safe, efficient, uniform and stable product quality and the controllable quality.

The invention discloses a quality control method for clear wulin pill. The method adopts the thin-layer qualitative detection of rheum officinale, berberine hydrochloride, Gardenoside and aristolochic acid, and also adopts the qualitative detection of the content of rheum emodin. Therefore, the method guarantees the safety and efficacy of medicines, improves the quality of products, and is convenient for standardization production of the products.

The invention discloses a detection method for a swelling-reducing and pain-alleviating tincture, which is used for controlling the content of aristolochic acid A in the swelling-reducing and pain-alleviating tincture and ensuring that the content of aristolochic acid A in the swelling-reducing and pain-alleviating tincture meets the safety requirement of a product. Due to the adoption of the method provided by the invention, the detection problem of a small amount of aristolochic acid A is solved, and a high-sensitivity, accurate and reliable detection method for aristolochic acid A is established. On the aspect of operation, the method provided by the invention is accurate and reliable, and has high accuracy and very high practical value.

The invention discloses a quality detection method for 29-componnet stagnation dissipation powder as a Tibetan medicinal composition and preparations thereof. IN the quality detection method, thin layer chromatography is used to carry out qualitative identification on frankincense, piperine and myristica fragrans in the 29-componnet stagnation dissipation powder, high performance liquid chromatography (HPLC) is used to carry out limit test on aconitine and costustoot aristolochic acid in the 29-componnet stagnation dissipation powder, and simultaneously, the high performance liquid chromatography is used to carry out quantitative detection on aloe-emodin, chrysophanol, rhein, emodin, emodin monomethyl ether and hydroxysafflor yellow A in the 29-componnet stagnation dissipation powder. According to the invention, the thin-layer identification method for the frankincense, the piperine and the myristica fragrans, the limit test method for the aconitine and the costustoot aristolochic acid, and the content determination method for the aloe-emodin, the chrysophanol, the rhein, the emodin, the emodin monomethyl ether and the hydroxysafflor yellow A are developed, so that the quality standard of a product is increased, and drug safety and effectiveness are guaranteed while the 29-componnet stagnation dissipation powder and the preparations thereof are used for treating diseases.

The method of detecting micro content of aristolochic acid A in water extract of Chinese medicine includes extracting the Chinese medicine sample with water to obtain water extract of Chinese medicine, treating the water extract by means of solid extracting technology to obtain sample solution, and high efficiency liquid chromatography detecting, with the stuffing for solid extracting being macroporous resin stuffing. The present invention has accurate determination of micro content of aristolochic acid A in water solution.

The invention discloses applications of benzopyran lactone in attenuating the toxicity of aristolochic acid and traditional Chinese medicines containing aristolochic acid. The benzopyran lactone provided by the invention can attenuate the renal toxicity of aristolochia acid, and can be used for preparing attenuation medicines for single traditional Chinese medicines, traditional Chinese medicinalextracts or traditional Chinese medicinal compound preparations containing aristolochic acid. Benzopyran lactone can be developed into single medicines to be taken together with the single traditionalChinese medicines, traditional Chinese medicinal extracts or traditional Chinese medicinal compound preparations containing aristolochic acid, or the single traditional Chinese medicines, the traditional Chinese medicinal extracts or the traditional Chinese medicinal compound preparations containing aristolochic acid can be developed into medicinal compositions with benzopyran lactone. The traditional Chinese medicines can be the traditional Chinese medicines, such as caulis aristolochiae manshuriensis, fructus aristolochiae, radix aristolochiae fangchi, radix aristolochiae, radix aristolochiae kaempferi, herba aristolochiae mollissimae, herba aristolochiae, cortex magnoliae officinalis and herba asari, which contain aristolochic acid. The traditional Chinese medicinal compound preparations can be the traditional Chinese medicinal compound preparations such as rheum officinale pills for clearing stomach heat, children-welfare tablets, lucidity-separating pills for treating five kindsof stranguria, gentian pills for purging liver-fire, resurrection pills containing 10 ingredients named with Chinese character 'Xiang', and styrax pills for treating coronary heart diseases, which contain aristolochic acid.

The invention relates to a method of removing the toxic ingredient of aristolochic acid from Traditional Chinese Medicine and the corresponding limit standard. Being aimed at the traditional Chinese medicinal materials containing aristolochic acid, the method is to obtain the processed medical herb in pieces with more than 99% f removal rate for the aristolochic acid after the process of repeated soaking - rinsing with weak acid strong alkali saline water and soaking - rinsing with clear water, draining and then vinegar baking. The invention is characterized in that the method can realize the purpose of selectively removing the toxic ingredient of aristolochic acid from the traditional Chinese medicine through the particular processing way, meanwhile, the limit standard of aristolochic acid is also formulated passing the systematical evaluation.

The invention discloses a tissue culture method for Aristolochia heterophylla. Aristolochia heterophylla is an Aristolochiaceae plant and can be harvested in the four seasons, preferably in autumn. After the root of Aristolochia heterophylla is dug out, silt and fibrous roots on the root of Aristolochia heterophylla are removed; then the root is dried to a semi-dry state in the sun; and if the root has a diameter of 2.5 cm or more, the root is halved and dried in the sun. The drug properties of Aristolochia heterophylla are that the root of Aristolochia heterophylla is cylindrical, has light brown or light brown-yellow cortex, and has a light grayish yellow color after the cortex is peeled; Aristolochia heterophylla is firm and not prone to breakage; a broken surface is uneven and spinous; a cross section is offwhite to yellow white; and Aristolochia heterophylla is mealy and has thick cortex and bitter and slightly astringent taste. Aristolochia heterophylla mainly comprises aristolochic acid, beta-sitosterol, magnoflorine, allantoin, etc. Aristolochia heterophylla is mainly used for dispelling wind, promoting urination, eliminating dampness and easing pain and applied to treatment of oedema in the head, face, arms and legs and pain in the waist and the knee joints. According to the invention, an Aristolochia heterophylla stem with a bud is used as an explant and subjected to bud induction, bud proliferation culture, rooting culture, domestication, transplanting and the like so as to realize in-vitro regeneration of Aristolochia heterophylla; so the tissue culture method provides technical support for mass breeding, rapid propagation and novel species promotion of Aristolochia heterophylla.

The invention relates to an asarum medicinal material and a quality control method of aristolochic acid A in a tincture for reducing swellings and easing pains, aiming to control the quality of aristolochic acid A in the asarum medicinal material and the tincture for reducing swellings and easing pains and guarantee that the content of the aristolochic acid A in the tincture for reducing swellings and easing pains meets the safety requirement of a product. The method solves the detection problem of trace aristolochic acid A, thus a high-sensitivity, accurate and reliable quality control method of the aristolochic acid A is established. Viewing from operations, the method has the advantages of simplicity, easiness of application, accuracy, reliability, high precision and higher practical value.

The invention relates to a traditional Chinese medicine preparation for treating cold as well as a preparation method and a detection method thereof. The traditional Chinese medicine preparation is prepared from the following crude drugs in parts by weight: 48 to 72 parts of tangerine peels, 880 to 1320 parts of Chinese ephedra herb, 112 to 168 parts of radix platycodonis, 24 to 36 parts of bulbus fritillariae uninbracteatae, 24 to 36 parts of fructus schisandrae chinensi, 16 to 24 parts of poria cocos, 16 to 24 parts of herba asari, 112 to 168 parts of chloriti lapis and 28 to 42 parts of liquorice roots. The detection method comprises qualitative detection of hyphae, the fructus schisandrae chinensi, aurantiamarin and the like and quantitative detection of aristolochic acid and ephedrine hydrochloride. According to the traditional Chinese medicine preparation, an improvement process is carried out so that the effect is more remarkable, the dose is reduced and the traditional Chinese medicine preparation is more convenient to take and carry, is rapidly released, is rapid in absorption and has higher bioavailability.

The invention relates to a process for removing toxic and harmful components from herbal medicines and extracting effective components from the herbal medicines, in particular to a process for preparing non-toxic extractives from asarum heterotropoides, belonging to the field of pharmaceutical technology. The preparation process for preparing the non-toxic extractives from the asarum heterotropoides comprises the following steps of: decocting a whole asarum heterotropoides plant by using hot water for extraction; cooling filer liquor; adding HCl and NaOH to regulate a pH value; and removing the toxic components, i.e. volatile safrole and nonvolatile components, such as aristolochic acid and aconitine, nor-aconitine and the like, out of asarum heterotropoides medicinal materials and effectively retaining the effective components through the purification steps of cation exchange resin, anion exchange resin, and the like. The invention can simultaneously remove multiple toxic and harmful components contained in the asarum heterotropoides medicinal materials together, obviously shorten the production period and reduce the production cost; in addition, a detoxification process has the advantages of strong compatibility, simple process, low energy consumption and resource saving without environment pollution in the production process.

The invention provides a Hirsutella sinensis fermented powder extract. The heavy metal content of the extract is lower than that in the international food hygiene standard, the extract does not have pesticide residue, can be completely dissolved in water or ethanol and does not contain culture medium residue, and the product is difficultly oxidized, difficultly generates smell of mutton, and has long retention period. The extract has obvious activity of resisting renal failure, 0.2 to 1.0g / Kg extract can obviously reduce serum creatinine (SCr) and blood urea nitrogen (BUN) content of a rat with renal failure, improves nephridial tissue degeneration caused by gentamicin, inverses renal tubular epithelial cell proliferation inhibition caused by aristolochic acid, reduces mRNA expression of renal failure key factors (TGF beta 1, TIMP-1, PAI-1 and ET) and has other effects; and compared with that of Hirsutella sinensis fermented powder, the effect of the Hirsutella sinensis fermented powder extract is improved by 2 to 10 times, the dosage can be greatly reduced, and the Hirsutella sinensis fermented powder extract is conveniently used by the patient and can be applied to preventing and treating renal failure disease. The Hirsutella sinensis fermented powder extract can serve as an active ingredient to be mixed with pharmaceutic excipients so as to prepare granules, capsules, tablets, buccal tablets, oral solution, tinctures and other formulations.

The invention relates to a potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood. The method mainly comprises the following steps: shearing birthwort branches into small sections, drying, pulverizing, performing ethanol extraction, performing petroleum ether and chloroform extraction, performing concentration, performing elution on the petroleum ether and the chloroform separately, performing separation and purification by a repeated column chromatography, precipitation and recrystallization method, and performing activity screening on the compound obtained by separation to obtain three compounds with nematocidal activity. By the method, the process flow is simple, the obtained three compounds with nematocidal activity serving as secondary metabolic products are potential and environment-friendly compounds for killing nematode (particularly meloidogyne incognita chitwood), the compounds can serve as leading compounds through activity evaluation comparison, and foundation is laid for further structure modification research.

The invention relates to application of ergot sterone in the preparation of medicaments for treating chronic renal failure. The ergot sterone has the effects of lowering urea nitrogen and serum creatinine, improving the renal function and reducing the pathological damage of adenine metabolites to the kidney. The invention provides a novel medicament for clinically treating chronic renal failure caused by adenine, aristolochic acid, rhubarb and the like.

The invention discloses an extraction process for aristolochic acid C. The extraction process comprises the following steps: 1) feeding radix aristolochiae into a crusher to be crushed to obtain crushed radix aristolochiae; 2) carrying out ethanol reflux extraction on the crushed radix aristolochiae twice so as to obtain an extraction solution; and 3) concentrating the extraction solution, addingtetrahydrofuran and ethyl acetate in a proportion of 2 to 8 into the concentrated solution, cooling the solution to 10 DEG C below zero, and putting the solution for 12-36 hours to crystallize to obtain the aristolochic acid C. By taking a novel mixed solvent tetrahydrofuran and ethyl acetate as a crystallizing solvent, the extraction rate of the aristolochic acid C is improved greatly, and the extraction process is simple and low in cost.

The invention relates to an extraction method of aristolochic acid A in a swelling dispersing and pain relieving tincture, and aims to solve the extraction problem of aristolochic acid A in the swelling dispersing and pain relieving tincture and meet the requirements for determining the content of aristolochic acid A in the swelling dispersing and pain relieving tincture. The method provided in the invention has the advantages of high extraction efficiency, reliable accuracy and very high practical value.

The invention relates to a method for preparing a traditional Chinese medicine, that is, a processing method of asarum. The method comprises the following steps: 1) the asarum is selected or the asarum cut into proper segments is selected; 2) the asarum is soaked once to twice in 5% ammonia water or saturated lime water solution which is ten times of the asarum, with every soaking lasting for 12 hours, the asarum is taken out and washed with water until the washing liquor is neutral, and then the asarum is dried in shade, thus the medicine is obtained. The toxic component aristolochic acid of the asarum can be removed by the processing method, the volatile oil content of the asarum can not be affected, and the method provides a new medicine for the reasonable clinical application of the asarum. The method is simple and reasonable, which has outstanding effect.

The invention discloses oral granules for treating the mumps and a preparation method thereof.The oral granules are prepared from codeine, aristolochic acid, robinin, syringic acid, paeonol, mint distillate, riboflavin, pentosans, nabumetone, nitrofurantoin, allantoin, water-soluble araboxylan, mixed amino acid, cyclodextrin and alcohol and other auxiliaries including sweetening agent, disintegrant and lubricant.Various western medicine components are reasonably combined, and the oral granules have functions of resisting infection, clearing away heat and toxic materials, and calming and stopping pain, can eliminate parotid gland swelling, resist virus infection and meanwhile enhance immunity of organism to promote the rapid rehabilitation of a patient, are easy to prepare and convenient to use and have quite good clinical application prospects.

The invention relates to a method for removing rigid structure matter from a sample. A nucleic acid-solid phase material and the to-be-treated sample are mixed or are in full contact at normal temperature, planar rigid structure molecules in the to-be-treated sample are combined with nucleic acid, the sample is separated out, and therefore the sample with the planar rigid structure molecules removed is obtained. The planar rigid structure molecules widely exist in the living environment, and for example, benzopyrene in edible oil, aflatoxin in fresh milk and agricultural and sideline products, a laboratory reagent ethidium bromide, aristolochic acid in Chinese herbal medicine and the like all have a planar rigid structure. The matter usually exists at the tiny content, but great health risks can be brought to people and other animals. The method is used for treating the sample containing the matter, therefore, the content of the planar rigid structure molecules in the sample is reduced to be minimal, the advantages of being high in efficiency, convenient to use, low in cost and the like are achieved, and the method is widely applied to the fields such as food, medicine and sewage treatment.

The invention discloses a method for enriching and detecting trace aristolochic acid A in a traditional Chinese medicine bolus of gentian for purging liver-fire. The method comprises the following steps: firstly, activating silica gel; then modifying a surface of the silica gel; synthesizing a molecularly imprinted polymer on the surface of the silica gel; filling a column with the molecularly imprinted polymer; activating with methanol to obtain a molecularly imprinted solid phase extraction column; using the molecularly imprinted solid phase extraction column for detecting and enriching aristolochic acid A in the traditional Chinese medicine bolus of gentian for purging liver-fire. The method provided by the invention has advantages of simple operation, high binding capacity, high mass transfer rate and high enrichment efficiency.

The invention relates to application of ergot sterone in the preparation of medicaments for treating chronic renal failure. The ergot sterone has the effects of lowering urea nitrogen and serum creatinine, improving the renal function and reducing the pathological damage of adenine metabolites to the kidney. The invention provides a novel medicament for clinically treating chronic renal failure caused by adenine, aristolochic acid, rhubarb and the like.

The present invention relates to an improved preparation of Yishen Juanbi Wan containing no aristolochine and its preparation method. The pharmacodynamic tests show that after the Chinese medicinal material hairy birthwort is removed from original prescription of Chinese medicine Yishen Juanbi Wan, the medicine effects of warming and recuperating the kidney-yang, tonifying kidney and strengthening bone, expelling pathogenic factors and removing obstruction is the channels, etc. of the Yishen Juanbi medicine composition prepared by said invention are not changed, so that said invention can reduce toxic side effect of its original prescription.

The invention relates to a coating technology, and aims to provide an anti-sliding coating. The anti-sliding coating consists of the following raw material components in parts by weight: 4-6 parts oftriethanolamine, 10-16 parts of magnesium hydroxide, 1-3 parts of bishydroxymethyl propionic acid, 6-8 parts of polyglutamic acid, 3-5 parts of liquid paraffin, 1-3 parts of aristolochic acid, 8-10 parts of rosin acid, 3-5 parts of carbon black, 10-12 parts of silicone acrylic emulsion, 10-12 parts of polyurethane emulsion and 20-44 parts of distilled water; the solid content of the silicone acrylic emulsion is 40%-60%; and the solid content of the polyurethane emulsion is 40%-60%. Compared with the prior art, the anti-sliding coating can directly coat the ground or a road surface; and after the anti-sliding coating is cured at a room temperature, an excellent dry-state and wet-state anti-sliding effect can be achieved. Compared with a coating with the same function, the coating has the advantage that the anti-sliding property is improved by 5 times or above.

The invention relates to an extraction method of aristolochic acid A in a swelling dispersing and pain relieving tincture, and aims to solve the extraction problem of aristolochic acid A in the swelling dispersing and pain relieving tincture and meet the requirements for determining the content of aristolochic acid A in the swelling dispersing and pain relieving tincture. The method provided in the invention has the advantages of high extraction efficiency, reliable accuracy and very high practical value.

The invention relates to a process for removing toxic and harmful components from herbal medicines and extracting effective components from the herbal medicines, in particular to a process for preparing non-toxic extractives from asarum heterotropoides, belonging to the field of pharmaceutical technology. The preparation process for preparing the non-toxic extractives from the asarum heterotropoides comprises the following steps of: decocting a whole asarum heterotropoides plant by using hot water for extraction; cooling filer liquor; adding HCl and NaOH to regulate a pH value; and removing the toxic components, i.e. volatile safrole and nonvolatile components, such as aristolochic acid and aconitine, nor-aconitine and the like, out of asarum heterotropoides medicinal materials and effectively retaining the effective components through the purification steps of cation exchange resin, anion exchange resin, and the like. The invention can simultaneously remove multiple toxic and harmfulcomponents contained in the asarum heterotropoides medicinal materials together, obviously shorten the production period and reduce the production cost; in addition, a detoxification process has the advantages of strong compatibility, simple process, low energy consumption and resource saving without environment pollution in the production process.

The invention discloses a method for enriching and detecting trace aristolochic acid A in a traditional Chinese medicine bolus of gentian for purging liver-fire. The method comprises the following steps: firstly, activating silica gel; then modifying a surface of the silica gel; synthesizing a molecularly imprinted polymer on the surface of the silica gel; filling a column with the molecularly imprinted polymer; activating with methanol to obtain a molecularly imprinted solid phase extraction column; using the molecularly imprinted solid phase extraction column for detecting and enriching aristolochic acid A in the traditional Chinese medicine bolus of gentian for purging liver-fire. The method provided by the invention has advantages of simple operation, high binding capacity, high mass transfer rate and high enrichment efficiency.

The invention provides a method for simultaneously detecting salicylic acid, aristolochic acid A, sodium cyclamate and beta-naphthol. According to the method, in the extraction process, the interference of partial interference substances in a sample to be detected to the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol is shielded; moreover, when mass spectrometric detection is carried out, the contents of the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol in the sample to be detected are more accurately tested in an electro-spraying ionization anion and multi-reaction monitoring scanning mode through quantitative determination of second-level ion fragments. Besides, by utilizing the method provided by the invention, the detection on the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol is simultaneously realized, the interference of similar compounds is eliminated, the accuracy and the reliability of the detected components are ensured, the detection sensitivity is high, the repeatability is good, and high accuracy and precision are achieved; the method is applicable to rapid quantitative screening and evaluation on the salicylic acid, the aristolochic acid A, the sodium cyclamate and the beta-naphthol in cigarette additives.