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Potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood

A technology of root knot nematode and aristolochic acid, applied in the directions of botanical equipment and methods, chemicals for biological control, nematicides, etc., to achieve the effects of convenient purchase, wide distribution, and simple and easy technological process

Active Publication Date: 2018-01-19
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through extensive literature research, there is currently no relevant report on the activity of Aristolochia plant materials against nematodes, nor is there any report on the activity of its biologically active components on nematodes, which may contribute to the development of a new class of plant properties. Discovery of insecticidal plant secondary metabolites

Method used

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  • Potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood
  • Potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood
  • Potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A potential green and effective separation and screening method for aristolochic acid and its derivatives compounds for preventing and controlling root-knot nematode incognita, comprising the following steps:

[0032] S1: Cut 10 kg of Aristolochia branches into small pieces, dry at 50°C for 10 hours, add 4L of methanol for extraction at room temperature, repeat 3 times, filter with suction, and evaporate the obtained filtrate at 40°C under reduced pressure to obtain methanol extract;

[0033] S2: Dissolve the methanol extract in 1L distilled water, add 1L petroleum ether for extraction, repeat 3 times; continue to extract the aqueous solution layer with 1L chloroform, repeat 3 times; evaporate under reduced pressure to obtain petroleum ether extract and chloroform extract ;

[0034]S3: Petroleum ether extract was mixed with 80-mesh coarse silica gel and separated on a 200-mesh fine silica gel column. Gradient elution, and finally the column was washed with methanol; si...

Embodiment 2

[0042] A potential green and effective separation and screening method for aristolochic acid and its derivatives compounds for preventing and controlling root-knot nematode incognita, comprising the following steps:

[0043] S1: Take 30kg of Aristolochia branches and cut them into small pieces, dry at 60°C for 8 hours, add 4L of methanol to extract at room temperature, repeat 5 times, filter with suction, and evaporate the obtained filtrate under reduced pressure at 55°C to obtain methanol extract;

[0044] S2: Dissolve the methanol extract in 3L distilled water, add 3L petroleum ether for extraction, repeat 5 times; continue to extract the aqueous solution layer with 3L chloroform, repeat 5 times; evaporate under reduced pressure to obtain petroleum ether extract and chloroform extract ;

[0045] S3: Petroleum ether extract was mixed with 60-mesh coarse silica gel and separated on a 300-mesh fine silica gel column. Gradient elution, and finally the column was washed with met...

experiment example 1

[0075] The compound with nematode-killing effect that is separated and screened in Examples and Comparative Examples is subjected to NMR analysis to obtain its structure:

[0076] A. in embodiment 1 and embodiment 2, all obtain three kinds of compounds with killing nematode effect, and structure is as follows:

[0077]

[0078] b. Comparative Example 1 only obtained the above-mentioned compound A which has the effect of killing nematodes, and Comparative Example 2 only obtained the above-mentioned compound B which has the effect of killing nematodes.

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Abstract

The invention relates to a potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood. The method mainly comprises the following steps: shearing birthwort branches into small sections, drying, pulverizing, performing ethanol extraction, performing petroleum ether and chloroform extraction, performing concentration, performing elution on the petroleum ether and the chloroform separately, performing separation and purification by a repeated column chromatography, precipitation and recrystallization method, and performing activity screening on the compound obtained by separation to obtain three compounds with nematocidal activity. By the method, the process flow is simple, the obtained three compounds with nematocidal activity serving as secondary metabolic products are potential and environment-friendly compounds for killing nematode (particularly meloidogyne incognita chitwood), the compounds can serve as leading compounds through activity evaluation comparison, and foundation is laid for further structure modification research.

Description

technical field [0001] The invention relates to the technical field of compound extraction and separation, in particular to a potential green separation and screening method for aristolochic acid and its derivative compounds that can effectively prevent and control root-knot nematode incognita. Background technique [0002] Plant parasitic nematodes (PPNs) cause an estimated US$157 billion in crop losses annually globally. Among them, root-knot nematodes (RKNs) are considered to be the major plant-parasitic nematode species in the world, affecting the quantity and quality of many annual and perennial plant species, such as tomato, pepper, watermelon and onion. Chemical insecticides are the traditional source of nematicides. However, the continuous use of chemical insecticides makes nematode populations gradually develop resistance to them, and also pollutes the environment and has adverse effects on humans, mammals and other non-target organisms; of course, the continuous u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/056C07D317/70A01N43/90A01N43/28A01P5/00G01N33/00
Inventor 董存柱卜淼淼秦鑫余森泉陈卜超王锡燕
Owner HAINAN UNIVERSITY
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