Potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood

A technology of root knot nematode and aristolochic acid, applied in the directions of botanical equipment and methods, chemicals for biological control, nematicides, etc., to achieve the effects of convenient purchase, wide distribution, and simple and easy technological process
CN107602575AActive Publication Date: 2018-01-19HAINAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HAINAN UNIVERSITY
Publication Date
2018-01-19

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Abstract

The invention relates to a potential and environment-friendly method for separating and screening aristolochic acid and derivative compounds thereof capable of effectively preventing and controlling meloidogyne incognita chitwood. The method mainly comprises the following steps: shearing birthwort branches into small sections, drying, pulverizing, performing ethanol extraction, performing petroleum ether and chloroform extraction, performing concentration, performing elution on the petroleum ether and the chloroform separately, performing separation and purification by a repeated column chromatography, precipitation and recrystallization method, and performing activity screening on the compound obtained by separation to obtain three compounds with nematocidal activity. By the method, the process flow is simple, the obtained three compounds with nematocidal activity serving as secondary metabolic products are potential and environment-friendly compounds for killing nematode (particularly meloidogyne incognita chitwood), the compounds can serve as leading compounds through activity evaluation comparison, and foundation is laid for further structure modification research.
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Description

technical field

[0001] The invention relates to the technical field of compound extraction and separation, in particular to a potential green separation and screening method for aristolochic acid and its derivative compounds that can effectively prevent and control root-knot nematode incognita. Background technique

[0002] Plant parasitic nematodes (PPNs) cause an estimated US$157 billion in crop losses annually globally. Among them, root-knot nematodes (RKNs) are considered to be the major plant-parasitic nematode species in the world, affecting the quantity and quality of many annual and perennial plant species, such as tomato, pepper, watermelon and onion. Chemical insecticides are the traditional source of nematicides. However, the continuous use of chemical insecticides makes nematode populations gradually develop resistance to them, and also pollutes the environment and has adverse effects on humans, mammals and other non-target organisms; of course, the continuous u...

Claims

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