Tissue culture method for Aristolochia heterophylla

A technique of tissue culture and self-prevention, which is applied in the field of plant tissue culture, can solve the problems of low survival rate, unfavorable promotion of good varieties, and differences in medicinal ingredients, and achieve the effect of high germination rate, easy operation, and good growth of seedlings

Inactive Publication Date: 2017-12-22
李正美
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, Hanzhong Fang has mainly adopted cuttings, but the survival rate is low, which is far from meeting the needs of large-scale production of Hanzhong Fang's...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] (1) Bud induction culture: take the Hanzhong anti-bud stem section as the explant, cut off the leaves, cut into 6 cm long sections with 3 axillary buds, soak them in washing powder solution for 5 minutes, and then wash them with soft hair Brush and gently scrub the surface of the explant to remove the dust and some bacteria on the surface, then rinse it with tap water for 1 hour, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 5 seconds, then wash it with sterile water for 3 times, and then wash it with 0.1 % mercuric chloride solution for 10 minutes, rinsed with sterile water for 5 times, and then dried the water drops on the surface with sterile filter paper, then inoculated into the bud induction medium for bud induction, and cultured at 26°C for 4 days in total darkness after inoculation. Then place it under 11 hours of light every day, and cultivate it under the condition of 1200lx light intensity for 28 days, count its bud inducti...

Embodiment 2

[0020] (1) Bud induction culture: take Hanzhong anti-bud stems as explants, cut off the leaves, cut into 3 long segments with 6 axillary buds, soak them in washing powder solution for 16 minutes, and then use a soft brush Gently brush the surface of the explant to remove the dust and some bacteria on the surface, then rinse it with tap water for 3 hours and place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 15 seconds, then wash it with sterile water for 6 times, and then wash it with 0.1% ethanol Mercuric chloride solution was sterilized for 15 minutes, rinsed with sterile water for 5 times, and then wiped dry the water drops on the surface with sterile filter paper, and then inoculated into bud induction medium for bud induction. Placed under the condition of 10 hours of light every day, and the light intensity was 1500lx, cultivated for 30 days, and counted its bud induction rate and growth situation, and the bud induction rate was 85%. The bud in...

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Abstract

The invention discloses a tissue culture method for Aristolochia heterophylla. Aristolochia heterophylla is an Aristolochiaceae plant and can be harvested in the four seasons, preferably in autumn. After the root of Aristolochia heterophylla is dug out, silt and fibrous roots on the root of Aristolochia heterophylla are removed; then the root is dried to a semi-dry state in the sun; and if the root has a diameter of 2.5 cm or more, the root is halved and dried in the sun. The drug properties of Aristolochia heterophylla are that the root of Aristolochia heterophylla is cylindrical, has light brown or light brown-yellow cortex, and has a light grayish yellow color after the cortex is peeled; Aristolochia heterophylla is firm and not prone to breakage; a broken surface is uneven and spinous; a cross section is offwhite to yellow white; and Aristolochia heterophylla is mealy and has thick cortex and bitter and slightly astringent taste. Aristolochia heterophylla mainly comprises aristolochic acid, beta-sitosterol, magnoflorine, allantoin, etc. Aristolochia heterophylla is mainly used for dispelling wind, promoting urination, eliminating dampness and easing pain and applied to treatment of oedema in the head, face, arms and legs and pain in the waist and the knee joints. According to the invention, an Aristolochia heterophylla stem with a bud is used as an explant and subjected to bud induction, bud proliferation culture, rooting culture, domestication, transplanting and the like so as to realize in-vitro regeneration of Aristolochia heterophylla; so the tissue culture method provides technical support for mass breeding, rapid propagation and novel species promotion of Aristolochia heterophylla.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a Hanzhong Fangji tissue culture method. Background technique [0002] Hanzhongfang is already an Aristolochiaceae plant, mainly produced in Hanzhong, Shaanxi. In addition, Hubei, Hunan, Sichuan, Yunnan and other places. It can be harvested in all seasons, but it is better to use the season diggers. After digging the roots, remove the silt and fibrous roots, scrape off the cork or not, sun-dry until half-dried, split in half with a diameter of more than 2.5cm, and dry in the sun. Properties of medicinal materials: the root is cylindrical, somewhat curved, with deep horizontal grooves at the curved part, 6-15cm long, and 1.5-3cm in diameter. The outer skin is light brown or light brownish yellow, and the outer skin is light grayish yellow when scraped off. The quality is solid, not easy to break, the section is uneven, spiny, the cross-section is off-...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李正美
Owner 李正美
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