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Method for removing rigid structure matter from sample

A rigid structure and plane technology, applied in the fields of food, medicine and sewage treatment, can solve the problems of lower removal efficiency and lower binding force between DNA and plane rigid molecules, and achieve the effects of low cost, low cost and simple operation.

Active Publication Date: 2017-08-11
GUANGXI UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such harsh conditions may cause premature denaturation of DNA molecules, thereby reducing the binding force of DNA to planar rigid molecules, thereby reducing the removal efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Using DNA-magnetic beads to remove benzopyrene from tea seed oil.

[0045] 1. Weigh 100g of crude tea seed oil, the content of benzopyrene is 16.1μg / kg after testing, and place the sample in a 250ml glass container with a sealed cover.

[0046] 2. Add 4-10ml of commercially available DNA-magnetic beads, and cover with a sealing cap.

[0047] 3. At room temperature, stir slowly with a magnetic stirrer at a stirring speed of 50 rpm for 1 hour. During this period, about every 10-20 minutes, pick up the container by hand and shake it upside down several times to promote full contact between the DNA-magnetic beads and the sample.

[0048] 4. After 1 hour, let it stand for about 1 minute to allow the DNA-magnetic beads to settle completely at the bottom of the container.

[0049] 5. Open the sealing cover and place it on the opening of the container with a magnetic frame. Most of the DNA-magnetic beads in the container are adsorbed on the magnetic frame. Leave o...

Embodiment 2

[0055] Example 2: Removal of ethidium bromide from aqueous solution using DNA-magnetic beads.

[0056] There are more than 2,000 colleges and universities of various types in my country, and there are tens of thousands of chemical laboratories of various types. Every day, a large number of teachers and students do various chemical experiments and generate a large amount of waste liquid. Among them, the most harmful pollutant is ethyl bromide ingot. In this example, a certain amount of ethidium bromide is added to a certain volume of water as a sample of polluted water, and the method of the present invention is used to remove the ethidium bromide, so as to demonstrate the practicability of the present invention in sewage treatment. This embodiment includes the following steps:

[0057] 1. Add ethidium bromide (EB) to 500 ml of purified water in a glass container with a tight lid to prepare a wastewater sample with an ethidium bromide content of 50 mg / l.

[0058] 2. Add 4-10ml...

Embodiment 3

[0067] Example 3: Using a DNA-dextran microbead column to remove aflatoxin B1 in a solution.

[0068] Since crops are often infected with Aspergillus flavus, agricultural by-products may contain aflatoxin B1, a highly toxic pollutant. This example demonstrates the method of the present invention for removing aflatoxin B1 from a sample. This embodiment includes the following steps:

[0069] 1. Prepare a commercially available aflatoxin B1 methanol standard in 30ml methanol / water (4 / 1, v / v) solution to prepare a 5μg / ml sample.

[0070] 2. Pack commercially available DNA-dextran microbeads into a column with a column height of 50 cm, and equilibrate at room temperature for 30 minutes.

[0071] 3. Wash the purification column twice with deionized water, 10 ml each time, and let the liquid flow out at a flow rate of 1-5 drops / second.

[0072] 4. Add the sample into the purification column, adjust the flow rate to 1-6 drops / second, until all the samples flow out of the purificati...

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PUM

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Abstract

The invention relates to a method for removing rigid structure matter from a sample. A nucleic acid-solid phase material and the to-be-treated sample are mixed or are in full contact at normal temperature, planar rigid structure molecules in the to-be-treated sample are combined with nucleic acid, the sample is separated out, and therefore the sample with the planar rigid structure molecules removed is obtained. The planar rigid structure molecules widely exist in the living environment, and for example, benzopyrene in edible oil, aflatoxin in fresh milk and agricultural and sideline products, a laboratory reagent ethidium bromide, aristolochic acid in Chinese herbal medicine and the like all have a planar rigid structure. The matter usually exists at the tiny content, but great health risks can be brought to people and other animals. The method is used for treating the sample containing the matter, therefore, the content of the planar rigid structure molecules in the sample is reduced to be minimal, the advantages of being high in efficiency, convenient to use, low in cost and the like are achieved, and the method is widely applied to the fields such as food, medicine and sewage treatment.

Description

technical field [0001] The invention relates to a technical method for removing planar rigid structure molecules from a sample, and is applicable to the fields of food, medicine, sewage treatment and the like. Background technique [0002] The planar rigid molecule refers to both the molecule whose whole molecule is a planar rigid configuration and the molecule whose partial structure is a planar rigid configuration. In these molecules, there are often one or more aromatic ring systems. These aromatic ring systems bind the atoms involved in a plane through a conjugated system (such as π-π conjugation), forming a rigid planar structure. [0003] This planar configuration and its hydrophobic nature enable the planar rigid molecule to interact with the DNA double-stranded helix. It is believed that many planar rigid molecules are capable of intercalating between adjacent pairs of base pairs in the DNA double helix and also binding into the major and minor grooves of DNA. Due...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/02B01D15/26B01D15/42
CPCB01D15/02B01D15/26B01D15/424
Inventor 孙庭广程谦伟孟陆丽刘昭明
Owner GUANGXI UNIVERSITY OF TECHNOLOGY
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