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Preparation for Hirsutella sinensis fermented powder extract and application of Hirsutella sinensis fermented powder extract in preventing and treating renal failure

A technology of Trichosporum chinensis and extract, which is applied in the field of traditional Chinese medicine, can solve the problems of small dosage, not easy to oxidize, and good solubility, and achieve the effects of reduced dosage, easy application, and long storage period

Inactive Publication Date: 2011-05-04
SHANGHAI ZHICAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the above-mentioned deficiencies, research and design a kind of Mortierella chinensis fermented bacterial powder extract, make its heavy metal content low, solubility is good, the product is not easy to oxidize, dosage is small, can be used for kidney Chinese patent medicine or health food for the prevention and treatment of exhaustion

Method used

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  • Preparation for Hirsutella sinensis fermented powder extract and application of Hirsutella sinensis fermented powder extract in preventing and treating renal failure
  • Preparation for Hirsutella sinensis fermented powder extract and application of Hirsutella sinensis fermented powder extract in preventing and treating renal failure
  • Preparation for Hirsutella sinensis fermented powder extract and application of Hirsutella sinensis fermented powder extract in preventing and treating renal failure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1. The preparation of Chinese Mortierella fermented bacteria powder extract

[0021] Chinese Mortierella fermented bacteria powder 100Kg, add deionized water (its conductivity is 15μ / cm)

[0022] 1000Kg, soaked for half an hour, reflux extraction in the traditional Chinese medicine extraction tank for 3 hours, extraction temperature: 95-100°C, and extract-1 after solid-liquid separation. Add 500Kg of deionized water to the residue, reflux extraction at 95-100°C for 2 hours, and extract-2 after suction filtration. Combine extract-1 and extract-2, and concentrate under reduced pressure in a rotary membrane vacuum to obtain a concentrate. The specific density of the concentrate was 1.04. The above concentrate was frozen at minus 18°C ​​for 24 hours, and then freeze-dried on a freeze dryer. Cold trap temperature: minus 55°C, vacuum degree: 30mtor, after drying for 24-36 hours, 20-30Kg of the water extract of Mortierella chinensis fermentation powder is obtaine...

Embodiment 2

[0024] The detection comparison of embodiment 2 Mortierella Chinese fermented bacterial powder extract and Chinese Mortierella fermented bacterial powder

[0025] Mortierella sinensis fermentation powder and the Mortierella sinensis fermentation powder extract (HsW) prepared in Example 1 were used as Cordyceps adenosine (calculated as adenosine), cordycepic acid (calculated as mannitol) and Cordyceps polysaccharide (calculated as mannitol) Dextran meter) for comparative determination. Results The content of the extract was 6.7, 8.6 and 3.0 times higher than that of the fermented bacteria powder. Table 1

[0026] The above extracts were then used for determination of pesticide residues and heavy metal content. Results No pesticides were detected in this product, and its heavy metal content was lower than the international food hygiene limit standard. Table 2

[0027] As determined by the fingerprint of traditional Chinese medicine recommended by the National Pharmacopoeia C...

Embodiment 3

[0033] Example 3. The improving effect of Mortierella chinensis fermented bacteria powder extract on blood creatinine and blood urea nitrogen in rats with renal failure

[0034] SD rats were randomly divided into 6 groups, 10 in each group. Be the test drug group of blank control group, gentamicin model group and three doses (Mertierella sinensis fermentation powder extract (HsW) 0.2g / Kg, 1.0g / Kg, 1.0g / Kg, 3.0g / Kg). Except for the blank control group, the other groups were injected intramuscularly with gentamicin (100mg / Kg) once a day for 7 consecutive days. At the same time as modeling, the drugs were administered in groups every day for a total of 8 days. On the 9th day, the rats were anesthetized with 3% Socram, blood was collected from the abdominal aorta, and the contents of blood urea nitrogen (BUN) and blood creatinine (SCr) were measured. The results showed that the BUN and SCr contents in the gentamicin model group were significantly higher than those in the normal...

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Abstract

The invention provides a Hirsutella sinensis fermented powder extract. The heavy metal content of the extract is lower than that in the international food hygiene standard, the extract does not have pesticide residue, can be completely dissolved in water or ethanol and does not contain culture medium residue, and the product is difficultly oxidized, difficultly generates smell of mutton, and has long retention period. The extract has obvious activity of resisting renal failure, 0.2 to 1.0g / Kg extract can obviously reduce serum creatinine (SCr) and blood urea nitrogen (BUN) content of a rat with renal failure, improves nephridial tissue degeneration caused by gentamicin, inverses renal tubular epithelial cell proliferation inhibition caused by aristolochic acid, reduces mRNA expression of renal failure key factors (TGF beta 1, TIMP-1, PAI-1 and ET) and has other effects; and compared with that of Hirsutella sinensis fermented powder, the effect of the Hirsutella sinensis fermented powder extract is improved by 2 to 10 times, the dosage can be greatly reduced, and the Hirsutella sinensis fermented powder extract is conveniently used by the patient and can be applied to preventing and treating renal failure disease. The Hirsutella sinensis fermented powder extract can serve as an active ingredient to be mixed with pharmaceutic excipients so as to prepare granules, capsules, tablets, buccal tablets, oral solution, tinctures and other formulations.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine. It specifically relates to the preparation method of Chinese Mortierella fermented powder extract and its application in preventing and treating renal failure. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis (Berk) G.H.Sung, J.M.Sung, Hywel-Jones & Spatafora) is a famous Chinese medicinal material. The medicinal material is the product of the fungus parasitizing the larvae of the bat moth. The "grass" of Cordyceps sinensis is the reproductive organ of the fungus - the sub-seat, and the mycelium in the dead body is its sclerotia. This kind of Cordyceps sinensis, which has both "worm" and "grass", cannot be cultivated artificially at present. However, the fungus isolated from Cordyceps sinensis can be fermented and cultivated by modern bioengineering technology, and the obtained product is the fermented bacteria powder of the fungus. The Latin scie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61K9/14A61K9/19A61P13/12A23L1/29A23L1/28A23L33/00
CPCA61K36/068A61K2236/19A61P13/12
Inventor 杨庆尧
Owner SHANGHAI ZHICAO BIOTECH
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