Two-dimensional liquid chromatography-mass spectrometry analysis method and system and storage medium

Abstract

The invention relates to a two-dimensional liquid chromatography-mass spectrometry analysis method and system and a storage medium, and the two-dimensional liquid chromatography-mass spectrometry analysis method comprises the following steps: obtaining type information of a sample introduced by a sample injector, and extracting corresponding preset separation time according to the type information; adjusting the communication between the sample injector and the plurality of chromatographic columns through a switching valve, and feeding a plurality of samples into the corresponding chromatographic columns for one-dimensional separation; according to preset separation time, sending the sample subjected to one-dimensional separation into a two-dimensional liquid phase port for two-dimensional separation, and supplementing a next sample to be analyzed into the chromatographic column; and sending the sample subjected to two-dimensional separation into a mass spectrometry detector for detection. According to the two-dimensional liquid chromatography-mass spectrometry analysis method and system and the storage medium, simultaneous treatment and continuous analysis of a plurality of samples are realized, and the flux of sample analysis is improved.

Description

Technical Field

[0001] This invention belongs to the field of analytical testing technology, and particularly relates to a two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method, system, and storage medium. Background Technology

[0002] In fields such as chemistry, biology, and pharmacy, liquid chromatography-mass spectrometry (LC-MS) systems are widely used as an important analytical tool for sample separation and identification. With the development of scientific research and production, the throughput requirements for sample analysis are becoming increasingly stringent.

[0003] Traditional liquid chromatography-mass spectrometry (LC-MS) systems use a single constant flow pump and a single chromatographic column for sample separation, which means they can only process one sample at a time, resulting in low analytical efficiency and making it difficult to meet the needs of rapid detection of large numbers of samples.

[0004] To improve analytical throughput, several multi-channel parallel liquid chromatography-mass spectrometry (LC-MS) systems have emerged in the current technology. However, these systems have shortcomings in column switching. Either the switching method is fixed and cannot be flexibly adjusted according to different sample separation times, or manual intervention is required, which not only increases operational complexity but also easily introduces errors, affecting the accuracy and reliability of the analytical results. Summary of the Invention

[0005] In view of this, the present invention provides a two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method, system and storage medium to solve the problem of how to improve analytical throughput in LC-MS analysis.

[0006] To achieve the above objectives, in a first aspect, the technical solution of the present invention to solve the technical problem is to provide a two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method, comprising: acquiring the type information of the sample introduced by the injector, and extracting the corresponding preset separation time according to the type information; adjusting the connection between the injector and multiple chromatographic columns by a switching valve, and sending multiple samples into the corresponding chromatographic columns for one-dimensional separation; according to the preset separation time, sending the sample that has completed one-dimensional separation into a two-dimensional liquid chromatography interface for two-dimensional separation, and adding the next sample to be analyzed to the chromatographic column; and sending the sample that has completed two-dimensional separation into a mass spectrometer detector for detection.

[0007] In one specific embodiment, when multiple samples are fed into corresponding chromatographic columns for one-dimensional separation, each chromatographic column is connected to a constant flow pump to provide the mobile phase for the chromatographic column.

[0008] In one specific embodiment, obtaining the type information of the sample introduced by the injector and extracting the corresponding preset separation time based on the type information includes: establishing a preset separation time database for each sample; obtaining the feature information of the sample introduced by the injector; and extracting the preset separation time of the introduced sample from the database based on the feature information.

[0009] In one specific embodiment, acquiring the feature information of the sample introduced by the injector includes: acquiring image information of the introduced sample; preprocessing the image information; and extracting features from the preprocessed image information.

[0010] In one specific embodiment, the step of adjusting the connection between the injector and multiple chromatographic columns by switching the valve to send multiple samples into the corresponding chromatographic columns for one-dimensional separation includes: determining the type of chromatographic column that is compatible with the preset separation time of the sample; detecting whether there is an idle chromatographic column among the columns of the compatible type; and driving the injector to connect with the idle chromatographic column by switching the valve.

[0011] In one specific embodiment, the step of sending the sample that has completed one-dimensional separation into a two-dimensional liquid chromatography interface for two-dimensional separation according to a preset separation time, and adding the next sample to be analyzed to the chromatographic column, includes: determining whether the sample has completed one-dimensional separation based on the preset separation time and the real-time monitored operating status of the chromatographic column; when it is determined that the sample has completed one-dimensional separation, a switching valve connects the chromatographic column to the two-dimensional liquid chromatography interface, so that the separated sample is delivered to the two-dimensional liquid chromatography interface for two-dimensional separation until the chromatographic column is in an idle state; the switching valve connects the injector to the idle chromatographic column, introducing the next sample to be analyzed into the chromatographic column.

[0012] In one specific embodiment, the step of sending the two-dimensionally separated sample into a mass spectrometer for detection includes: after the separated sample is transported to the mass spectrometer, a detection start signal is sent to the mass spectrometer to control the mass spectrometer to perform ionization, mass analysis and signal acquisition on the sample according to preset detection parameters; the mass spectrometer generates the detection data of the sample and stores and records it.

[0013] Secondly, the present invention also provides a two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis system, comprising: an extraction module for acquiring the type information of the sample introduced by the injector and extracting the corresponding preset separation time according to the type information; a switching module for adjusting the connection between the injector and multiple chromatographic columns through a switching valve, thereby sending multiple samples into the corresponding chromatographic columns for one-dimensional separation; a sample replenishment module for sending the sample that has completed one-dimensional separation into the two-dimensional liquid chromatography interface for two-dimensional separation according to the preset separation time, and replenishing the next sample to be analyzed into the chromatographic column; and a detection module for sending the sample that has completed two-dimensional separation into a mass spectrometer detector for detection.

[0014] In one specific embodiment, the switching valve includes a valve body, multiple interfaces, and a drive mechanism. The multiple interfaces are respectively connected to an injector, multiple chromatographic columns, and a two-dimensional liquid chromatography interface. The drive mechanism is used to drive the valve body to move and realize the connection between different interfaces.

[0015] Thirdly, the present invention also provides a computer-readable storage medium having a computer program stored thereon, wherein the computer program, when executed by a processor, implements the method described thereon.

[0016] Compared with existing technologies, the two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method, system, and storage medium provided by this invention have the following advantages: By utilizing multiple chromatographic columns in parallel operation, combined with switching valves, simultaneous processing and continuous analysis of multiple samples are achieved, increasing the throughput of sample analysis. Furthermore, the automatic switching of the injector connection to different columns based on sample separation time ensures that samples with different separation times are separated on appropriate columns, improving adaptability to different sample types. The column switching process requires no manual intervention, reducing operation time and errors. Simultaneously, by rationally arranging the separation order of samples on different columns, the overall analysis time is shortened, improving analytical efficiency. Accurate and reliable column switching reduces the impact of human factors on analytical results, ensuring accuracy and repeatability. Attached Figure Description

[0017] Figure 1 A schematic diagram illustrating the application environment of the two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method provided by this invention; Figure 2 This is a flowchart of the two-dimensional liquid chromatography-mass spectrometry analysis method provided in the first embodiment of the present invention; Figure 3 Flowchart of steps for obtaining sample type information and corresponding preset separation time; Figure 4 Flowchart of steps for obtaining sample feature information; Figure 5 Flowchart of steps for adjusting sample entry into the chromatographic column using a switching valve; Figure 6 Flowchart of steps for adjusting the sample entry into the two-dimensional liquid chromatography interface by switching the valve and replenishing the sample into the idle chromatographic column; Figure 7 This is a flowchart illustrating the steps involved in testing the separated samples. Detailed Implementation

[0018] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0019] It should be noted that all directional indications in the embodiments of this application are only used to explain the relative positional relationship and movement of each component in a specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0020] Furthermore, the use of terms such as "first" and "second" in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. Additionally, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of a person skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, the user should consider such a combination of technical solutions to be non-existent and not within the scope of protection claimed in this application.

[0021] The two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method provided in the first embodiment of this application can be applied to, for example... Figure 1 In the illustrated environment, the sample enters through an injector, passes through a switching valve, and then enters a chromatographic column for initial one-dimensional separation. The switching valve allows the injector to switch between different chromatographic columns, enabling the sample to enter each column individually. A constant flow pump is connected to each column to provide the mobile phase. The sample, initially separated by the column, enters a two-dimensional chromatography system via a two-dimensional liquid chromatography interface for further two-dimensional separation. Finally, after separation, the sample is detected by a mass spectrometer.

[0022] In one embodiment, such as Figure 2 As shown, the two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method includes: S100: Obtain the type information of the sample introduced by the injector, and extract the corresponding preset separation time based on the type information; The S200 uses a switching valve to adjust the connection between the injector and multiple chromatographic columns, allowing multiple samples to be sent into the corresponding chromatographic columns for one-dimensional separation. S300, according to the preset separation time, sends the sample that has completed one-dimensional separation into the two-dimensional liquid interface for two-dimensional separation, and adds the next sample to be analyzed to the chromatographic column; The S400 sends the two-dimensionally separated sample into a mass spectrometer for detection. In this embodiment, the sample type introduced each time is first determined based on the sample information from the injector. Then, the preset separation time required for the corresponding sample to undergo initial one-dimensional separation in the chromatographic column is obtained according to the sample type. Next, the switching valves are adjusted sequentially so that multiple samples enter multiple chromatographic columns simultaneously for one-dimensional separation. After each sample undergoes one-dimensional separation in the chromatographic column for the preset time, the separated samples are sequentially introduced into the two-dimensional chromatography system through the two-dimensional liquid chromatography interface for two-dimensional separation. Simultaneously, after a sample completes one-dimensional separation and elutes from the column, the injector simultaneously introduces the next sample to be analyzed, ensuring the continuity of one-dimensional separation and improving analytical throughput. Finally, the two-dimensional separated samples are sequentially sent to the mass spectrometer detector for detection and analysis, completing the sample analysis process.

[0023] In some embodiments, when multiple samples are fed into corresponding chromatographic columns for one-dimensional separation, each chromatographic column is connected to a constant flow pump to provide the mobile phase to the chromatographic column.

[0024] It should be noted that peristaltic pumps, reciprocating infusion pumps, etc., can also be used, as long as the flow rate and delivery of the mobile phase can be stably controlled to meet the basic conditions for one-dimensional separation.

[0025] In some embodiments, such as Figure 3-4 As shown, obtaining the sample type information introduced by the injector and extracting the corresponding preset separation time based on the type information includes: S110, Establish a database of preset separation times for each sample; S120, acquire characteristic information of the sample introduced by the injector; S130, extract the preset separation time of the introduced sample from the database based on the feature information; In this embodiment, a preset separation time corresponding to each sample is pre-established and stored. When a sample is introduced through the injector, the introduced sample is identified, the characteristic information of the introduced sample is obtained, and the corresponding preset separation time is extracted from the database based on the characteristic information.

[0026] It should be noted that the database uses feature information as labels, and each label is associated with a corresponding preset separation time. The feature information can be appearance features obtained through image recognition, or barcodes / QR codes set on the sample container. The sample category is obtained by scanning, and the feature information is mapped to the labels in the database to extract the preset separation time of the sample introduced by each injector.

[0027] Furthermore, obtaining characteristic information about the sample introduced by the injector includes: S121, Obtain image information of the introduced sample; S122, Preprocess the image information; S123, extract features from the preprocessed image information; It should be noted that the image information can be a color image of the label area of ​​the sample container captured by a camera. Preprocessing of the image information can be done in any way, such as cropping the image based on edge detection algorithms (e.g., the Canny operator) to retain the region of interest where the label is located, removing background interference, removing image noise through Gaussian filtering to improve clarity, converting the color image to the HSV color space, and extracting the hue (H) and saturation (S) feature parameters of the label's background color. Feature extraction can be performed on the preprocessed image, using shape recognition algorithms (e.g., Hough transform) to extract the contour features of geometric patterns (the side length ratio of triangles, the stroke structure of the internal digit "3"), and combining this with the HSV parameters of the background color to form a comprehensive feature vector.

[0028] After obtaining the sample's characteristic information through the above steps, the preset separation time for introducing the sample can be obtained by matching the tags in the database through the mapping relationship.

[0029] To illustrate with a specific example: First, a pre-defined separation time database is established and stored in the control system. The database uses sample characteristic information as labels, with each label corresponding to a unique pre-defined separation time. The specific format is as follows:

[0030] When sample 1 (human serum metabolite sample) is introduced into the sample container, a high-definition camera (image acquisition device) captures an image of the label area, obtaining raw image information. After image preprocessing (cropping the label area, removing background noise, and enhancing text clarity), feature information is extracted from the image: the label is blue, circular in shape, and bears the text "Serve Sample 1," meaning the acquired feature information is "blue circular label + 'Serve Sample 1' text label." This feature information is compared with feature information labels in the database, and the corresponding label is matched through a mapping relationship. This determines the preset separation time for the sample, which is 10 minutes, serving as the basis for subsequent valve switching actions.

[0031] Similarly, when sample 2 (drug intermediate A sample) is introduced, the feature information extracted from the image acquisition is "red square label + 'intermediate 2' text identifier", and the preset separation time after matching the database is 8 minutes; when sample 3 (natural product extract B sample) is introduced, the preset separation time after matching the feature information is 12 minutes.

[0032] In some embodiments, such as Figure 5As shown, by adjusting the connection between the injector and multiple chromatographic columns through the switching valve, multiple samples are sent into the corresponding chromatographic columns for one-dimensional separation, including: S210, Determine the type of chromatographic column that is compatible with the preset separation time of the sample; S220, detects whether there is an idle column in the compatible chromatographic column; S230, the switching valve drives the injector to connect to the idle chromatographic column; It should be noted that the model and specifications of the chromatographic column can be selected according to the separation requirements of different samples. Whether the chromatographic column is idle can be determined by any means such as pressure sensor or system log recording. The switching valve can be used to connect or disconnect the injector from each chromatographic column by means of stepper motor or other means.

[0033] To illustrate with a specific example: When the injector introduces sample 1, a biological sample containing aflatoxin M1 (such as milk), with a preset separation time of 10 minutes, according to the "metabolite-separation time" rule, the compatible chromatographic column A is ChromCore C18 (120 Å, 5 μm). Column A achieves efficient retention of polar substances through single-point bonding technology. At this time, column A (ChromCore C18) provides real-time column pressure data via a pressure sensor. The current column pressure is 3.2 MPa (normal operating pressure range 2-8 MPa), and the UV detector has no signal output, indicating an idle state. The switching valve connects the injector to column A to deliver sample 1 into column A for preliminary one-dimensional separation.

[0034] When sample 2 is an amino acid sample derivatized by OPA / FMOC (such as a pharmaceutical intermediate), with a preset separation time of 8 minutes, the matched column B is an Agilent Poroshell HPH-C18 column. Its porous packing material is stable under high pH conditions, making it suitable for the separation of basic amino acids. The autosampler interface of column B (HILIC-Z) displays a "Ready" status, and the system log records its last run time as 30 minutes ago, confirming it is in an idle state. A switching valve connects the injector to column B to deliver sample 2 into column B for preliminary one-dimensional separation. In some embodiments, such as Figure 6 As shown, according to the preset separation time, the sample that has completed one-dimensional separation is sent to the two-dimensional liquid chromatography interface for two-dimensional separation, and the next sample to be analyzed is added to the chromatographic column, including: S310 determines whether the sample has completed one-dimensional separation based on the preset separation time and the real-time monitored column operating status. S320: When the sample is determined to have completed one-dimensional separation, the switching valve connects the chromatographic column to the two-dimensional liquid chromatography interface, so that the separated sample is transported to the two-dimensional liquid chromatography interface for two-dimensional separation until the chromatographic column is idle. S330, the switching valve connects the injector to the idle column, introducing the next sample to be analyzed into the column; To illustrate with a specific example, when the sample to be separated is a pharmaceutical intermediate sample with a preset separation time of 8 minutes, and one-dimensional separation is being performed in chromatographic column A, the running time of chromatographic column A is tracked in real time. When the timer reaches 8 minutes and the detector returns a separation signal indicating the end, it is determined that the sample has completed one-dimensional separation. A switching valve connects chromatographic column A to the two-dimensional liquid chromatography interface, allowing the separated sample to be transported to the two-dimensional liquid chromatography interface for two-dimensional separation until chromatographic column A is idle. At this point, the switching valve reconnects the injector to chromatographic column A, allowing the injector to introduce the next sample to be analyzed into chromatographic column A for another one-dimensional separation.

[0035] In some embodiments, such as Figure 7 As shown, the process of sending the two-dimensionally separated sample into a mass spectrometer for detection includes: S410: After the separated sample is transported to the mass spectrometer detector, a detection start signal is sent to the mass spectrometer detector to control the mass spectrometer detector to perform ionization, mass analysis and signal acquisition of the sample according to the preset detection parameters. S420, the mass spectrometer detector generates, stores, and records the sample detection data; Taking a drug metabolite sample (such as aflatoxin metabolite) as a specific example, the analytical detection process is explained as follows: After separation by a two-dimensional chromatography system, the drug metabolite sample (such as aflatoxin metabolite) is transported to the mass spectrometer detector via a two-dimensional liquid chromatography interface. Based on the preset detection parameters for this sample type (such as an electrospray ionization source voltage of 5000V and a scan range of m / z 100-500), a start signal is sent to the mass spectrometer detector. After receiving the sample, the mass spectrometer detector converts the sample molecules into ions through electrospray ionization. After separation by the mass analyzer, the detector collects the ion signals, generating an ion chromatogram and mass-to-charge ratio data. After detection, the mass spectrometer detector transmits the data to the control system, which automatically stores the detection results for subsequent qualitative or quantitative analysis.

[0036] A second embodiment of the present invention provides a two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis system, comprising: The extraction module is used to obtain the type information of the sample introduced by the injector and extract the corresponding preset separation time according to the type information; The switching module is used to adjust the connection between the injector and multiple chromatographic columns through the switching valve, so that multiple samples can be sent into the corresponding chromatographic columns for one-dimensional separation. The sample replenishment module is used to send the sample that has completed one-dimensional separation into the two-dimensional liquid chromatography interface for two-dimensional separation according to the preset separation time, and to replenish the next sample to be analyzed into the chromatographic column. The detection module is used to send the two-dimensionally separated sample into a mass spectrometer for detection.

[0037] In this embodiment, the injector is used to introduce the sample into the two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis system. Multiple constant flow pumps are connected to different chromatographic columns to provide mobile phase for the columns. Multiple chromatographic columns are used for one-dimensional separation of the sample. A switching valve is used to control the sample entering different chromatographic columns and to transport the separated sample to the two-dimensional liquid chromatography interface. The two-dimensional liquid chromatography interface is used to introduce the sample separated by the one-dimensional chromatographic columns into the two-dimensional chromatography system for further separation. A mass spectrometer detector is used to detect the sample after two-dimensional separation.

[0038] The switching valve includes a valve body, multiple interfaces, and a drive mechanism. The multiple interfaces are respectively connected to the injector, multiple chromatographic columns, and two-dimensional liquid chromatography interface. The drive mechanism is used to drive the valve body to move and realize the connection between different interfaces.

[0039] It should be noted that the operation of the two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis system is controlled by a control system, which is electrically connected to the injector, multiple constant flow pumps, switching valves, the 2D liquid chromatography interface, and the mass spectrometry detector to control the operation of the entire 2D LC-MS analysis system.

[0040] The control system has preset separation time parameters for different samples. When a sample is introduced by the injector, the control system controls the switching valve to switch the sample to the corresponding chromatographic column for separation according to the preset separation time for that sample. During the sample separation process, the control system monitors the operating status of each chromatographic column in real time. When a chromatographic column completes sample separation, the control system controls the intelligent column switching valve to connect the column to the two-dimensional liquid chromatography interface, delivering the separated sample to the two-dimensional liquid chromatography interface. At the same time, the system switches the newly introduced sample to an idle chromatographic column for separation.

[0041] A third embodiment of the present invention provides a computer-readable storage medium having a computer program stored thereon, which, when executed by a processor, implements the steps in the above-described method embodiments.

[0042] Those skilled in the art will understand that all or part of the processes in the above embodiments can be implemented by a computer program instructing related hardware. The computer program can be stored in a non-volatile computer-readable storage medium, and when executed, it can include the processes of the embodiments described above. Any references to memory, databases, or other media used in the embodiments provided in this application can include at least one of non-volatile and volatile memory. Non-volatile memory can include read-only memory (ROM), magnetic tape, floppy disk, flash memory, optical memory, high-density embedded non-volatile memory, resistive random access memory (ReRAM), magnetic random access memory (MRAM), ferroelectric random access memory (FRAM), phase change memory (PCM), graphene memory, etc. Volatile memory can include random access memory (RAM) or external cache memory, etc. By way of illustration and not limitation, RAM can take many forms, such as Static Random Access Memory (SRAM) or Dynamic Random Access Memory (DRAM). The databases involved in the embodiments provided in this application may include at least one type of relational database and non-relational database. Non-relational databases may include, but are not limited to, blockchain-based distributed databases. The processors involved in the embodiments provided in this application may be general-purpose processors, central processing units, graphics processing units, digital signal processors, programmable logic devices, quantum computing-based data processing logic devices, etc., and are not limited to these. The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described; however, as long as the combination of these technical features does not contradict each other, it should be considered within the scope of this specification.

[0043] Compared with existing technologies, the two-dimensional liquid chromatography-mass spectrometry (LC-MS) analysis method, system, and storage medium provided by this invention utilizes multiple chromatographic columns operating in parallel, combined with switching valves, to achieve simultaneous processing and continuous analysis of multiple samples, thereby increasing sample throughput. Furthermore, the automatic switching of the injector connection to different chromatographic columns based on sample separation time allows samples with different separation times to be separated on appropriate columns, improving adaptability to different sample types. The elimination of manual intervention in the column switching process reduces operation time and errors. Simultaneously, by rationally arranging the separation order of samples on different chromatographic columns, the overall analysis time is shortened, improving analytical efficiency. Accurate and reliable column switching reduces the impact of human factors on analytical results, ensuring accuracy and repeatability.

[0044] The specific embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made in accordance with the technical concept of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A two-dimensional liquid chromatography-mass spectrometry analysis method, characterized by, The method comprises the following steps: acquiring type information of samples introduced by the sample injector, and extracting preset separation time corresponding to the type information according to the type information; adjusting the communication between the sample injector and multiple chromatographic columns through a switching valve, and sending multiple samples into corresponding chromatographic columns for one-dimensional separation; according to the preset separation time, sending the samples completing one-dimensional separation into a two-dimensional liquid chromatography interface for two-dimensional separation, and supplementing the next sample to be analyzed into the chromatographic column; sending the samples completing two-dimensional separation into a mass spectrometer for detection.

2. The method according to claim 1, wherein: when the multiple samples are sent into the corresponding chromatographic columns for one-dimensional separation, a constant flow pump is connected to each chromatographic column for providing a mobile phase for the chromatographic column.

3. The method according to claim 1, wherein the two-dimensional liquid chromatography-mass spectrometry analysis method is a two-dimensional liquid chromatography-mass spectrometry method. The step of acquiring type information of samples introduced by the sample injector, and extracting preset separation time corresponding to the type information according to the type information comprises the following steps: establishing a database of preset separation time corresponding to each sample; acquiring characteristic information of the samples introduced by the sample injector; extracting the preset separation time of the introduced sample from the database according to the characteristic information.

4. The method according to claim 3, wherein the two-dimensional liquid chromatography-mass spectrometry analysis method is a two-dimensional liquid chromatography-mass spectrometry method. The step of acquiring characteristic information of the samples introduced by the sample injector comprises the following steps: acquiring picture information of the introduced sample; preprocessing the picture information; extracting features from the preprocessed picture information.

5. The method according to claim 1, wherein the method is a two-dimensional liquid chromatography-mass spectrometry method. The step of adjusting the communication between the sample injector and multiple chromatographic columns through a switching valve, and sending multiple samples into corresponding chromatographic columns for one-dimensional separation comprises the following steps: determining the type of the chromatographic column suitable for the preset separation time of the sample; detecting whether there is an idle chromatographic column in the chromatographic column of the determined type; driving the switching valve to connect the sample injector with the idle chromatographic column.

6. The method according to claim 5, wherein the two-dimensional liquid chromatography-mass spectrometry analysis method is a two-dimensional liquid chromatography-mass spectrometry method. The step of according to the preset separation time, sending the samples completing one-dimensional separation into a two-dimensional liquid chromatography interface for two-dimensional separation, and supplementing the next sample to be analyzed into the chromatographic column comprises the following steps: judging whether the sample completes one-dimensional separation according to the preset separation time and the real-time monitored running state of the chromatographic column; when it is determined that the sample completes one-dimensional separation, driving the switching valve to connect the chromatographic column with the two-dimensional liquid chromatography interface, so that the separated sample is transported to the two-dimensional liquid chromatography interface for two-dimensional separation until the chromatographic column is in an idle state; driving the switching valve to connect the sample injector with the chromatographic column in the idle state, and introducing the next sample to be analyzed into the chromatographic column.

7. The method according to claim 1, wherein the method is a two-dimensional liquid chromatography-mass spectrometry method. The step of sending the samples completing two-dimensional separation into a mass spectrometer for detection comprises the following steps: after the separated sample is transported to the mass spectrometer, sending a detection start signal to the mass spectrometer to control the mass spectrometer to ionize, mass analyze and collect signals of the sample according to preset detection parameters; the mass spectrometer generates detection data of the sample and stores and records the detection data.

8. A two-dimensional liquid chromatography-mass spectrometry system, characterized by, The method comprises the following steps: a extraction module is configured to acquire type information of samples introduced by the sample injector, and extract preset separation time corresponding to the type information according to the type information; a switching module is configured to adjust the communication between the sample injector and multiple chromatographic columns through a switching valve, and send multiple samples into corresponding chromatographic columns for one-dimensional separation; a sample supplement module is configured to according to the preset separation time, send the samples completing one-dimensional separation into a two-dimensional liquid chromatography interface for two-dimensional separation, and supplement the next sample to be analyzed into the chromatographic column. The detection module is configured to send the sample after two-dimensional separation into a mass spectrometer for detection.

9. The two-dimensional liquid chromatography-mass spectrometry analysis method of claim 8, wherein: The switching valve comprises a valve body, a plurality of interfaces, and a driving mechanism. The plurality of interfaces are respectively connected to the sample injector, the plurality of chromatographic columns, and the two-dimensional liquid chromatography interface. The driving mechanism is configured to drive the valve body to actuate and realize the communication between different interfaces.

10. A computer-readable storage medium having stored thereon a computer program, characterized in that, The computer program, when executed by a processor, implements the method of any one of claims 1 to 7.

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