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Method for simultaneously measuring Amadori compounds in tobaccos

A determination method and compound technology, applied in the field of simultaneous determination of 9 kinds of Amadori compounds in tobacco, can solve the problems of difficulty in co-detection of such substances, inaccurate characterization of high-performance liquid chromatography, etc., and achieve strong anti-interference ability, Qualitatively accurate effects

Active Publication Date: 2014-01-08
HONGTA TOBACCO GRP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High performance liquid chromatography is not accurate enough, and it is difficult to achieve the co-detection of such substances

Method used

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  • Method for simultaneously measuring Amadori compounds in tobaccos
  • Method for simultaneously measuring Amadori compounds in tobaccos
  • Method for simultaneously measuring Amadori compounds in tobaccos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] a. Crush the tobacco leaf sample, pass through a 40-mesh sieve, weigh 0.5-2g sample, place it in a stoppered conical flask, add 10-50mL methanol-water solution (50%-70%) for extraction, filter, and collect the filtrate;

[0026] b. Purification: add 10-30mL n-hexane to the filtrate and extract 2-5 times to remove impurities such as pigments, then concentrate the methanol-water phase to dryness, dilute to 10-50mL with methanol-water solution, and pass through a 0.22μm organic filter membrane , to prepare the sample;

[0027] c. Determination: HPLC-MS / MS is used for analysis, positive ion ionization mode, multiple reaction monitoring scanning, and the operating conditions are the same as the above technical scheme.

Embodiment 2

[0029] a. Tear apart the cigarette sample, pour out the shredded tobacco and crush it, pass through a 40-mesh sieve, weigh 0.5-2g sample, put it in a stoppered conical flask, add 10-50mL methanol-water solution (50%-70%) for extraction , filter, and collect the filtrate;

[0030] b. Purification: add 10-30mL n-hexane to the filtrate and extract 2-5 times to remove impurities such as pigments, then concentrate the methanol-water phase to dryness, dilute to 10-50mL with methanol-water solution, and pass through a 0.22μm organic filter membrane , to prepare the sample;

[0031] c. Determination: HPLC-MS / MS is used for analysis, positive ion ionization mode, multiple reaction monitoring scanning, and the operating conditions are the same as the above technical scheme.

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Abstract

The invention discloses a method for simultaneously measuring Amadori compounds in tobaccos, relates to a method for measuring important ingredients in tobaccos, and particularly relates to a method for simultaneously measuring nine Amadori compounds in tobaccos by the aid of liquid chromatography-tandem mass spectrometry. The method includes steps of 1, sample extracting: smashing tobacco samples, extracting the tobacco samples by methanol-aqueous solution, filtering the tobacco samples and collecting filter liquor; 2, purifying: adding normal hexane into the filter liquor, repeatedly extracting the filter liquor, removing impurities such as coloring matters in the filter liquor, concentrating the filter liquor by methanol-aqueous phases until the filter liquor is dry, diluting the filter liquor by methanol-aqueous solution until the volume of the diluted filter liquor meets requirements, and filtering the filter liquor by an organic filter membrane to obtain test samples; 3, measuring: analyzing the test samples via HPLC-MS / MS (high-performance liquid chromatography-mass spectrometry / mass spectrometry), and performing multi-reaction monitoring scanning on the test samples in a cation ionization mode. The method has the advantages that the Amadori compounds in complicated systems of the tobaccos can be effectively extracted and analyzed and can be detected jointly, the sensitivity and the interference resistance are high, and the method has popularization and application value.

Description

technical field [0001] The invention relates to a method for determining important components of tobacco, in particular to the simultaneous determination of nine kinds of Amadori compounds in tobacco by means of liquid chromatography-tandem mass spectrometry. Background technique [0002] The Amadori compound formed by amino acid and glucose is an intermediate product of the non-enzymatic browning reaction of tobacco, and is an important non-volatile flavor precursor formed in the process of tobacco conditioning, aging and processing. Relevant studies have shown that the content of such substances in tobacco has an important impact on the smoking quality of tobacco. Research on the distribution of such substances in tobacco has guiding significance for cigarette formulation and tobacco processing. [0003] Due to the difficulty in extracting and separating the Amadori compounds in tobacco, it is one of the chemical components of tobacco that are difficult to accurately deter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 朱瑞芝白晓莉岳保山向能军孟昭宇
Owner HONGTA TOBACCO GRP
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