Method for quickly, qualitatively and quantitatively measuring Lactobacillus casei in probiotic dairy products

A technology for the qualitative determination of Lactobacillus casei, applied in the direction of microbial-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve the lack of stability and reliability of test results, limited detection efficiency, and time-consuming Power and other issues
CN101712989AInactive Publication Date: 2010-05-26INNER MONGOLIA AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INNER MONGOLIA AGRICULTURAL UNIVERSITY
Publication Date
2010-05-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for quickly, qualitatively and quantitatively measuring Lactobacillus casei in probiotic dairy products. The method comprises the following steps: aiming at the Lactobacillus casei in probiotic dairy products, independently designing a species specificity primer of the 16S rRNA gene sequence of the Lactobacillus casei, carrying out species specificity PCR and real-time fluorescent quantitative PCR reaction by using the primer, and analyzing through an agarose gel electrophoresis pattern and a real-time fluorescent quantitative PCR pattern, thereby establishing the method for simply, conveniently, quickly, accurately, qualitatively and quantitatively measuring Lactobacillus casei in probiotic dairy products.
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Description

【Technical field】

[0001] The invention relates to the technical field of dairy products containing probiotics. More specifically, the present invention relates to a method for rapid qualitative and quantitative determination of Lactobacillus casei in probiotic milk products. 【Background technique】

[0002] Probiotic dairy products have become a hot topic due to their rich nutritional value and special beneficial effects. At present, there are many kinds of probiotics and dairy products containing probiotics on the market, and they are growing at a high rate every year. The supervision and certification of its quality, especially in terms of qualitative and quantitative detection, lacks scientific, reasonable and rapid methods. The qualitative and quantitative detection of probiotics in the traditional method still adopts the methods of physiological and biochemical reactions and selective medium pure culture isolation and counting. Traditional methods are easily affected ...

Claims

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