Lignocellulose anaerobic methane-producing pretreatment and fermentation technology

A technology of lignocellulose and fermentation process, applied in the field of lignocellulose anaerobic fermentation, can solve problems such as difficult biodegradation, secondary pollution, etc., and achieve the effects of enhancing cellulase content, no secondary pollution, and short time

Inactive Publication Date: 2016-04-20
TONGJI UNIV
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Problems solved by technology

However, acid-base pretreatment will produce waste liquid that is difficult to biodegrade, which contains substances such as furfural and furan that inhibit the activity of anaerobic microorganisms, which will cause secondary pollution

Method used

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  • Lignocellulose anaerobic methane-producing pretreatment and fermentation technology
  • Lignocellulose anaerobic methane-producing pretreatment and fermentation technology
  • Lignocellulose anaerobic methane-producing pretreatment and fermentation technology

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Embodiment 1

[0030] The embodiment adopts rice straw as the substrate of this process. For specific implementation process steps, see figure 1 .

[0031] 1) The collected rice straw has a high moisture content, and it is placed in a glass greenhouse for drying. After drying, the moisture content is about 6.5%;

[0032] 2) Use a screw extrusion crusher to crush the dried rice straw, sieve after crushing, sieve out the materials with a particle size larger than 1cm and then crush them again, and collect the materials with a particle size smaller than 1cm;

[0033] 3) Add cellulase to the broken rice straw, the amount of cellulase added is about 200U per gram of cellulose. In this example, the cellulose content of rice straw is 36.8%, so the amount of cellulase added is about 74U / g. Fully mix cellulase with straw;

[0034] 4) Add acetic acid-sodium acetate buffer solution with a pH of 5.0 to the mixture of cellulase and rice straw, and use the buffer solution to adjust the moisture conten...

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Abstract

The invention relates to a lignocellulose anaerobic methane-producing pretreatment and fermentation technology. Pretreatment is conducted on lignocellulose through cellulase, and hydrolysis of the lignocellulose in the anaerobic fermentation process is facilitated. The technology comprises the steps that the moisture content of the raw materials is adjusted to below 10%; physical crushing is conducted on the lignocellulose through a crushing machine; the cellulase is added into the crushed lignocellulose; a buffer solution is added into lignocellulose and cellulase mixed liquor, and the solid content is controlled to blow 10%; a reaction is conducted for 72 h at 50 DEG C, and full stirring is conducted; an anaerobic fermentation inoculum is directly added into the lignocellulose and cellulase mixed liquor to be fermented, and the additive amount of dry matter of the inoculum is 50%-300% of that of the dry matter of the lignocellulose. According to the lignocellulose anaerobic methane-producing pretreatment and fermentation technology, the content of the cellulase inside a reactor can be increased, the content of the cellulase in the anaerobic fermentation process is increased, the efficiency of anaerobic fermentation is promoted, and the daily gas yield, the accumulated gas yield and the unit raw material gas yield are increased.

Description

technical field [0001] The invention relates to the technical field of lignocellulose anaerobic fermentation, including anaerobic biogas pretreatment and fermentation process for wastes containing lignocellulose components such as agricultural straw, garden garbage, energy crops and the like. Background technique [0002] In recent years, the impact of energy depletion and environmental pollution caused by the extensive use of fossil energy on daily life has become increasingly prominent. Using biomass to produce renewable energy is one of the feasible ways to alleviate these two problems. [0003] Lignocellulose is a biomass resource that widely exists in nature. Through anaerobic fermentation, the cellulose and hemicellulose contained in lignocellulose can be converted into biogas. Using lignocellulose anaerobic fermentation biogas technology can recover energy on the basis of solving the problem of agricultural straw waste and garden waste disposal. In addition, energy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P5/02
CPCC12P5/023C12P19/14C12P2203/00Y02E50/30
Inventor 周雪飞张亚雷顾禹李琪
Owner TONGJI UNIV
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