Method for improving activity of lignin-degrading enzyme generated by phanerochaete chrysosporium after enzyme activity decay

A technology of lignin-degrading enzyme and Phanerochaete protothecoides, which is applied in the biological field, can solve the problem of not seeing high-level activity of lignin-degrading enzyme, and achieve the effect of simple condition control

Inactive Publication Date: 2016-04-27
TSINGHUA UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

So far, in the study of lignin-degrading enzymes produced by Phanerochaete chrysosporium, no method has been found that can effectively maintain a high level of lignin-degrading enzyme activity or re-enhance the enzyme activity after the enzyme activity decays. Stable production is still the main bottleneck problem to be solved for the practical application of white rot fungi and lignin-degrading enzymes

Method used

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  • Method for improving activity of lignin-degrading enzyme generated by phanerochaete chrysosporium after enzyme activity decay
  • Method for improving activity of lignin-degrading enzyme generated by phanerochaete chrysosporium after enzyme activity decay
  • Method for improving activity of lignin-degrading enzyme generated by phanerochaete chrysosporium after enzyme activity decay

Examples

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Effect test

Embodiment 1

[0030] Embodiment 1, Phanerochaete chrysosporium improves enzyme activity after lignin degrading enzyme activity decays under carbon-limited culture

[0031] The method of improving the activity of Phanerochaete chrysosporium after the activity of lignin degrading enzymes decays under carbon-limited culture is to add 2g / L glucose after the carbon source is exhausted during the fermentation process of Phanerochaete chrysosporium to realize yellowing. Phanerochaete sporogenes increased lignin-degrading enzyme activity under carbon-limited culture after attenuation, as follows:

[0032] 1. Preparation of carbon-limited liquid medium

[0033] A carbon-limited liquid medium was used, and the medium was slightly modified based on the typical Tien and Kirk medium (1988). The C / N ratio (molar ratio) of the medium was 3.8, and the specific composition was as follows:

[0034] The carbon-limited liquid medium consists of a final concentration of 5.04g / L (28mM) glucose, a final concentrat...

Embodiment 2

[0046] Example 2, Phanerochaete chrysosporium improves enzyme activity after lignin degrading enzyme activity decays under carbon-limited culture

[0047] The method of improving the activity of Phanerochaete chrysosporium after the activity of lignin degrading enzymes decays under carbon-limited culture is to add 5g / L glucose after the carbon source is exhausted during the fermentation process of Phanerochaete chrysosporium to realize yellowing. Phanerochaete sporogenes increased lignin-degrading enzyme activity under carbon-limited culture after attenuation, as follows:

[0048] 1, the preparation of carbon-limited liquid culture medium: same as embodiment 1;

[0049] 2. Maintain the stability of lignin-degrading enzyme activity produced by Phanerochaete chrysosporium

[0050] Basically the same as Example 1, the only difference is the step of adding glucose:

[0051] Ferment and cultivate the above-mentioned spore-inoculated culture system in an air environment (not addit...

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Abstract

The invention discloses a method for improving the activity of lignin-degrading enzyme generated by phanerochaete chrysosporium after enzyme activity decay. The method comprises the following steps: fermenting the phanerochaete chrysosporium in a carbon-limiting culture medium, and adding glucose to a fermentation system when a carbon source is used up, so that the activity of the lignin-degrading enzyme generated by the phanerochaete chrysosporium is improved after decay. According to the method, the recovery of the lignin-degrading enzyme activity can be realized only by adding an appropriate amount of glucose to the culturing system, the conditions are easy to control, and the method is suitable for the stable production and practical degradation of the lignin-degrading enzyme generated by phanerochaete chrysosporium.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for increasing the enzyme activity of Phanerochaete chrysosporium lignin degrading enzyme after its activity decays. Background technique [0002] White rot fungus is a kind of higher filamentous fungus, most of which belong to Basidiomycetes in classification, grow on trees or wood, and get its name because it causes woody white rot. White-rot fungi degrade lignin because they can produce lignin-degrading enzymes and secrete them outside the cells. The degradation of lignin by lignin-degrading enzymes is a chain reaction process based on free radicals, which has strong oxidation and substrate non-specificity. This degradation mechanism enables lignin-degrading enzymes to not only degrade lignin, but also degrade Many xenobiotics and persistent toxic organic pollutants in the environment (Barr & Aust, 1994; Camero et al., 2000; Gao et al., 2010). Therefore, white-r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08C12R1/645
CPCC12N9/0065C12Y111/01013C12Y111/01014
Inventor 喻国策文湘华王建龙
Owner TSINGHUA UNIV
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