Method for improving activity of lignin-degrading enzyme generated by phanerochaete chrysosporium after enzyme activity decay
A technology of lignin-degrading enzyme and Phanerochaete protothecoides, which is applied in the biological field, can solve the problem of not seeing high-level activity of lignin-degrading enzyme, and achieve the effect of simple condition control
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Embodiment 1
[0030] Embodiment 1, Phanerochaete chrysosporium improves enzyme activity after lignin degrading enzyme activity decays under carbon-limited culture
[0031] The method of improving the activity of Phanerochaete chrysosporium after the activity of lignin degrading enzymes decays under carbon-limited culture is to add 2g / L glucose after the carbon source is exhausted during the fermentation process of Phanerochaete chrysosporium to realize yellowing. Phanerochaete sporogenes increased lignin-degrading enzyme activity under carbon-limited culture after attenuation, as follows:
[0032] 1. Preparation of carbon-limited liquid medium
[0033] A carbon-limited liquid medium was used, and the medium was slightly modified based on the typical Tien and Kirk medium (1988). The C / N ratio (molar ratio) of the medium was 3.8, and the specific composition was as follows:
[0034] The carbon-limited liquid medium consists of a final concentration of 5.04g / L (28mM) glucose, a final concentrat...
Embodiment 2
[0046] Example 2, Phanerochaete chrysosporium improves enzyme activity after lignin degrading enzyme activity decays under carbon-limited culture
[0047] The method of improving the activity of Phanerochaete chrysosporium after the activity of lignin degrading enzymes decays under carbon-limited culture is to add 5g / L glucose after the carbon source is exhausted during the fermentation process of Phanerochaete chrysosporium to realize yellowing. Phanerochaete sporogenes increased lignin-degrading enzyme activity under carbon-limited culture after attenuation, as follows:
[0048] 1, the preparation of carbon-limited liquid culture medium: same as embodiment 1;
[0049] 2. Maintain the stability of lignin-degrading enzyme activity produced by Phanerochaete chrysosporium
[0050] Basically the same as Example 1, the only difference is the step of adding glucose:
[0051] Ferment and cultivate the above-mentioned spore-inoculated culture system in an air environment (not addit...
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