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Method for degrading gallic acid in wastewater by using Phanerochaete chrysosporium

A technology of Phanerochaete and gallic acid, applied in the field of bioengineering, can solve problems affecting water color, taste and smell, teratogenicity, carcinogenicity, etc.

Inactive Publication Date: 2014-03-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The main components of dissolved organic matter (DOM) in natural water bodies are natural organic acids (Natural organic acids, NOA), and 15-30% of natural organic acids are hydrophilic small molecule organic acids, and gallic acid is a natural organic acid. The ubiquitous hydrophilic small molecule organic acids will bring the following problems in the drinking water purification process: 1) affect the color, taste and smell of water; 2) form disinfection byproducts (Disinfection byproducts, DPBs for short), such as chloroform and haloacetic acids, which can cause damage to organisms, especially human liver, kidney and central nervous system, and have carcinogenic, teratogenic and mutagenic effects after they are combined with chlorine in water; 3) Membrane fouling will be easily caused in the subsequent advanced water treatment process
[0003] At present, there are several patents on the treatment of waste water containing gallic acid, but the main methods are water washing or alkali extraction, such as the patented gallic acid production waste residue recycling method (application number 200610086067.7), which protects the waste residue from washing twice at 90~100 degrees , the method of recovering gallic acid by two pressing methods; the patented gallic acid mother liquor recovery process (patent number 200910226622.5) protects the process of extracting gallic acid with alkali, and then adjusting the pH8.0-9.0 to precipitate to obtain crude gallic acid; patented gallic acid A new technology for the recovery and treatment of waste water, waste residue and waste carbon from acid production (patent application number 201210469098.6) discloses the process of recovering gallic acid from the waste residue and waste carbon produced by gallic acid by secondary washing and extrusion in countercurrent; Method for producing waste liquid (application number 2010136553.1) and the article "Fenton reaction-neutralization-biological treatment of gallic acid crude waste liquid" (Journal "Water Treatment Technology" 2010, Volume 36, Issue 9, pp106-109), Disclosed is a method in which the crude gallic acid waste liquid is subjected to catalytic oxidation, pH adjustment, precipitation, activated carbon adsorption, and finally dilution and aeration treatment. These methods all have incomplete treatment or are diluted with a large amount of water.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of cultures on test tube slant

[0023] A ring of Phanerochaete chrysosporium strains ( Phanerochaete chrysosporium ) (CCGMC5.776), 26°C, cultured for 50 hours; stored at 4°C for later use.

[0024] 2. Preparation of cultured strains in liquid shake flasks

[0025] Accurately weigh 5 grams of glucose, 0 grams of peptone, 5 grams of yeast extract, 0.2 grams of magnesium sulfate, 0.5 grams of Tween 80, 2 grams of potassium dihydrogen phosphate, 1000 mL of water, pH 5, and pack into 250 mL Erlenmeyer flasks, each containing 50 grams, a total of 20 bottles, sterilized at 120°C for 40 minutes; after cooling, each bottle was inoculated with 3 slant cells of Phanerochaete chrysosporium test tubes (5×5mm), and cultivated at 25°C, 150 rpm, for 72 hours;

[0026] 3. Preparation of primary liquid culture

[0027] Accurately weigh 50 grams of glucose, 0 grams of peptone, 50 grams of yeast extract, 2 grams of magnesium sulfate, 5 grams of Tween 80, 20 grams of pota...

Embodiment 2

[0031] 1. Preparation of cultures on test tube slant

[0032] Insert a ring of Phanerochaete chrysosporium strains into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technical Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) (China Agricultural Microbiological Strain Preservation Management Center ACCC 30942), 30°C, incubation time 100 hours; store at 7°C for later use.

[0033] 2. Preparation of cultured strains in liquid shake flasks

[0034] Accurately weigh 150 grams of glucose, 25 grams of peptone, 25 grams of yeast extract, 0.4 grams of magnesium sulfate, 4 grams of Tween 80, 5 grams of potassium dihydrogen phosphate, 10 L of water, pH 6, and pack into 250 mL Erlenmeyer flasks, each 100 grams, a total of 100 bottles, sterilized at 130°C for 30 minutes; after cooling, inoculate each bottle with 4 pieces of Phanerochaete chrysosporium test tube slant (5×5mm), and cultivate for 50 hours at 30°C, 150 rpm ;

[0035] 3. Pre...

Embodiment 3

[0041] 1. Preparation of cultures on test tube slant

[0042] Insert a ring of Phanerochaete chrysosporium strain into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technical Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367) China Industrial Microbiology Culture Collection and Management Center CICC14067), 35°C, incubation time 144 hours; store at 10°C for later use.

[0043] 2. Preparation of cultured strains in liquid shake flasks

[0044] Accurately weigh 300 grams of glucose, 50 grams of peptone, 0 grams of yeast extract, 10 grams of magnesium sulfate, 100 grams of Tween 80, 90 grams of potassium dihydrogen phosphate, 10 L of water, pH 7.5, and pack in 250 mL triangular flasks, each with 120 mL, a total of 80 bottles, sterilized at 140°C for 20 minutes; after cooling, inoculate each bottle with 5 slant cells of Phanerochaete chrysosporium test tubes (5×5 mm), and culture at 35°C, 150 rpm, for 72 hours;

[0045] 3. Prepa...

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PUM

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Abstract

The invention discloses a technique for degrading gallic acid in wastewater by using Phanerochaete chrysosporium, relating to the field of bioengineering. The technique comprises the following steps: by using Phanerochaete chrysosporium as the initial strain, carrying out liquid shake culture and liquid strain amplification culture, transferring the strain solution into the wastewater containing gallic acid, supplementing certain nutrient substances, and carrying out fermentation culture. By using the technique, the removal rate of the gallic acid can reach 60-100%.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to using Phanerochaete chrysosporium to degrade waste water containing gallic acid. After concentrating or diluting the original waste water until the content of gallic acid is 1-50 g / L, the components of the culture medium are supplemented, and Phanerochaete chrysosporium strains are added to degrade the gallic acid in the waste water. After degradation by Phanerochaete chrysosporium, the removal rate of gallic acid can reach 60-100%. Background technique [0002] The main components of dissolved organic matter (DOM) in natural water bodies are natural organic acids (Natural organic acids, NOA), and 15-30% of natural organic acids are hydrophilic small molecule organic acids, and gallic acid is a natural organic acid. The ubiquitous hydrophilic small molecule organic acids will bring the following problems in the drinking water purification process: 1) affect the color, tas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/14C12R1/645C02F101/34
Inventor 张志才
Owner JIANGSU UNIV
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