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Phanerochaete chrysosporium mycelium growthform controlling method

A technology for the growth of Phanerochaete chrysosporium and hyphae, which is applied in the field of bioengineering, can solve the problems affecting the yield and activity of useful metabolites, restricting the application of Phanerochaete chrysosporium, reducing dissolved oxygen and mass transfer rates, and achieving The effect is remarkable, the cost is low, and the effect of improving the adsorption rate

Inactive Publication Date: 2015-02-04
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The filamentous fungus—Phanerochaete chrysosporium has a long fermentation period. As the fermentation time increases, the shape and number of mycelial balls change and the accumulation of extracellular metabolites causes the dissolved oxygen and mass transfer rate to decrease during the fermentation process. , thereby affecting the yield and activity of useful metabolites
This severely restricts the application of Phanerochaete chrysosporium

Method used

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  • Phanerochaete chrysosporium mycelium growthform controlling method
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  • Phanerochaete chrysosporium mycelium growthform controlling method

Examples

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Effect test

Embodiment 1

[0030] A method for controlling Phanerochaete chrysosporium mycelium growth form, it comprises the steps:

[0031] (1) Strain activation: Phanerochaete chrysosporium was inoculated on the plate medium for activation culture, the culture temperature was 37°C, and after 2-3 days of culture, it was transferred to room temperature for culture until the mycelia covered the plate;

[0032] (2) Adding inorganic nanoparticles: Based on 1L liquid medium, add 0.01g of TiO 2 Nanoparticles, the particle diameter of the nanoparticles is 10nm, so as not to add the TiO 2 The medium of nanoparticles is used as a comparative example;

[0033] (3) Cultivation of bacteria: prepare the activated strain obtained in step (1) into a spore suspension, and inoculate it into the obtained in step (2) with TiO 2 Nanoparticle-based liquid medium supplemented with TiO per liter 2 The spore inoculum in the liquid medium of nanoparticles was 2.0×10 6 ~3.0×10 6 one, cultured on a shaking table at 30° C.,...

Embodiment 2

[0042] The method for controlling the growth morphology of Phanerochaete chrysosporium mycelium in this embodiment is roughly the same as in Example 1, except that: 1) Based on 1L liquid medium, add 5g of SiO 2 Nanoparticles, the particle size of the nanoparticles is 500nm; 2) The conditions of fermentation culture are: culture on a shaking table at 32° C., the speed of the shaking table is 145 rpm, and cultivate for 5 to 7 days.

[0043] Carry out the test of dye decolorization rate and adsorption rate according to the method identical with embodiment 1. like Figure 5 As shown, after adding crystal violet dye to the fermentation broth, the spherical shape of Phanerochaete chrysosporium mycelium in this example is clearly visible. Within 6 hours, the decolorization rate of the dye increased from 21% to 70%, an increase of about 3.5 times; within 1 hour, the adsorption rate of the dye increased from 15% to 70%, an increase of about 4.5 times.

Embodiment 3

[0045] The method for controlling the mycelial growth morphology of Phanerochaete chrysosporium in this embodiment is roughly the same as in Example 1, except that: 1) Based on 1L of liquid medium, add 10g of Al 2 o 3 Nanoparticles, the particle size of the nanoparticles is 800nm; 2) The conditions of the fermentation culture are: culture on a shaking table at 33° C., the speed of the shaking table is 140 rpm, and cultivate for 5 to 7 days.

[0046] Carry out the test of dye decolorization rate and adsorption rate according to the method identical with embodiment 1. like Figure 6 As shown, after adding crystal violet dye to the fermentation broth, the spherical shape of Phanerochaete chrysosporium mycelium in this example is clearly visible. Within 6 hours, the dye decolorization rate increased from 21% to 49%, an increase of about 2.3 times; within 1 hour, the dye adsorption rate increased from 15% to 48%, an increase of about 3.3 times.

[0047] Known from embodiment 1 t...

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Abstract

The invention relates to a phanerochaete chrysosporium mycelium growthform controlling method and belongs to the field of bioengineering. The phanerochaete chrysosporium mycelium growthform controlling method comprises the following steps: adding inorganic nanoparticles to a fluid nutrient medium of the phanerochaete chrysosporium to carry out ferment cultivation, and obtaining a mycelium group of the phanerochaete chrysosporium due to the facts that the inorganic nanoparticles react with spores and mycelia, and the inorganic nanoparticles enter into mycelial beads. The mycelium group has a good application effect in dye decolorization.

Description

technical field [0001] The invention belongs to the field of biological engineering and relates to a method for controlling the growth form of Phanerochaete chrysosporium mycelium. Background technique [0002] Phanerochaete chrysosporium ( Phanerochaete chrysosporium ) is a class of filamentous basidiomycete white-rot fungi that contain an excellent lignin decomposition system. This excellent lignin decomposition system consists of a series of extracellular peroxidases, typical enzymes such as lignin peroxidase and manganese peroxidase. These extracellular peroxidases are highly non-specific in the oxidation of substrates, and can degrade a variety of organic substances, including natural polymers, reducing compounds, oxidizing compounds, and toxic compounds. Therefore, Phanerochaete chrysosporium has a wide application value in many biotechnology fields, such as the treatment of hazardous waste, the bioremediation of polluted land, the preparation of biofuel, biopulping ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12R1/645
Inventor 苏宝连李其昌李昱谢浩
Owner WUHAN UNIV OF TECH
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