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Method for controlling growth morphology of mycelia

A technology of mycelium and morphology, applied in the field of bioengineering, can solve the problems of inability to effectively control the fermentation morphology of filamentous fungi, complicated operation methods, harsh process conditions, etc., and is suitable for large-scale fermentation, easy to scale up production, operation simple effect

Active Publication Date: 2017-05-10
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In summary, the prior art has harsh process conditions and complicated operation methods, which cannot solve the problem of effectively controlling the fermentation morphology of filamentous fungi

Method used

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  • Method for controlling growth morphology of mycelia
  • Method for controlling growth morphology of mycelia

Examples

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Embodiment 1

[0054] This embodiment illustrates the method of utilizing farnesol to control mycelium morphology:

[0055] (1) Preparation of medium: basal medium includes, glucose 20g / L, (NH 4 ) 2 SO 4 5g / L, KH 2 PO 4 4g / L, MgSO 4 7H2O 2g / L, FeSO 4 7H2O 0.1g / L, CaCl 2 2H 2 O 0.5g / L, glucose is sterilized separately, sterilized at 115°C for 30min;

[0056] (2) Seed culture: add 1 mL of Rhizopus niger spore suspension to the medium described in step (1), cultivate it for 20 hours at 28° C. and 160 rpm, and obtain the seed liquid after the culture is homogenized;

[0057] (3) Farnesol is dissolved in ethanol at a concentration of 200 mM, and 0.22 μm membrane filter is sterilized to prepare a farnesol solution;

[0058] (4) At the 0th hour of the whole culture process, add farnesol solution until the concentration in the fermentation broth reaches 200 μM, and the culture medium without adding farnesol is used as a control;

[0059] (5) Fermentation culture: the seed liquid was inse...

Embodiment 2

[0062] This embodiment illustrates a method utilizing farnesol to control mycelium morphology:

[0063] (1) The preparation of the medium: the basic medium includes, sucrose 50g / L, urea 10g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 1g / L, CaCl 2 2H 2 O 0.05g / L, glucose is sterilized separately, sterilized at 115°C for 30min;

[0064] (2) Seed culture: add 1 mL of Aspergillus niger spore suspension to the medium described in step (1), cultivate at 30° C. and 150 rpm for 36 hours, and homogenize the culture to become the seed liquid;

[0065] (3) Farnesol is dissolved in ethanol at a concentration of 200 mM, and 0.22 μm membrane filter is sterilized to prepare a farnesol solution;

[0066] (4) At the 0th hour of the whole culture process, add farnesol solution until the concentration in the fermentation broth reaches 150 μM, and the culture medium without adding farnesol is used as a control, and the pH is adjusted to 6.0 with HCl;

[0067] (5) Fermentation culture: the seed liqui...

Embodiment 3

[0070] This example illustrates a method of using farnesol to control mycelium growth morphology to carry out lovastatin fermentation production:

[0071] (1) Preparation of medium: basal medium includes, glycerol 5%, glucose 10g / L, soybean peptone 2g / L, (NH 4 ) 2 SO 4 2g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 1g / L, FeSO 4 ·7H 2 O0.05g / L, CaCl 2 2H 2 O0.05g / L, glucose is sterilized separately, sterilized at 115°C for 30min;

[0072] (2) Seed culture: add 1 mL of Monascus spore suspension to the medium described in step (1), cultivate at 30° C. and 200 rpm for 48 hours, and homogenize the culture to become the seed liquid;

[0073] (3) Farnesol is dissolved in ethanol at a concentration of 200 mM, and 0.22 μm membrane filter is sterilized to prepare a farnesol solution;

[0074] (4) At the 48th hour of the entire culture process, add the farnesol solution in step (3) until the concentration in the fermentation broth reaches 500 μM, and the culture medium without addin...

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Abstract

The invention relates to the technical field of bioengineering and particularly relates to a method for controlling the growth morphology of mycelia. The method comprises the following steps: at a mycelium growth phase of a filamentous fungus fermentation process, adding farnesol into a fermented solution; and continually fermenting to obtain spherical filamentous fungus mycelia, wherein the final concentration of added farnesol is 150-1000 microns. According to the method provided by the invention, the growth morphology in the filamentous fungus fermentation process can be efficiently and conveniently controlled and the fermentation yield of filamentous fungi is improved; and the method is simple to operate and high in universality and is suitable for being amplified in industrial production.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for controlling the growth form of mycelia. Background technique [0002] Filamentous fungi are widely used in industrial production, and their products cover industrial enzyme preparations (such as amylase, cellulase, protease, lipase, etc.), organic acids, antibiotics, etc. In industrial production, submerged fermented filamentous fungi usually have three forms: agglomerates, flocs and globules. Different forms of filamentous fungi will directly affect the physical parameters and substrate transfer in the fermentation system, so the growth form is very important for the production of fermentation products and the control of the fermentation process. The growth form of filamentous fungi has a significant impact on cell metabolic pathways, product accumulation, and separation and extraction. Therefore, how to control the growth form of filamentous fungi and maxim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/66C12R1/845C12R1/885C12R1/645
CPCC12N1/14
Inventor 刘春朝王科峰郭晨
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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