The inventive method of producing a eukaryotic
viral vector comprises contacting a
eukaryotic cell, which comprises a unique
enzyme that nicks or cleaves
a DNA molecule, with a recombinant phage vector, or contacting a
eukaryotic cell, which does not comprise a unique
enzyme that nicks or cleaves
a DNA molecule, simultaneously or sequentially, in either order, with (i) a unique
enzyme that nicks or cleaves
a DNA molecule, and (ii) a recombinant phage vector. The recombinant phage vector comprises the
DNA molecule comprising (a) a eukaryotic
viral vector genome comprising a coding sequence, (b) a phage packaging site that is not contained within the eukaryotic
viral vector genome, and (c) a
promoter that is operably linked to the coding sequence.Alternatively, the
DNA molecule is not present within the recombinant phage vector. The
eukaryotic cell is contacted with the first
DNA molecule and a recombinant phage vector. The first DNA molecule comprises a replication deficient eukaryotic viral vector
genome comprising at least one adenoviral
inverted terminal repeat and a packaging
signal. The recombinant phage vector comprises a second DNA molecule and a phage packaging site, wherein the second DNA molecule complements in trans the replication deficient eukaryotic viral vector genome.The DNA molecule(s) enter the eukaryotic
cell, and the unique enzyme nicks or cleaves the DNA molecule in the eukaryotic
cell in at least one region not contained within the eukaryotic viral vector genome, thereby inducing the production of and ultimately producing a eukaryotic viral vector.