22 results about "Molecular recombination" patented technology

The application relates to preparation and application of a giant Eimeria nanosubunit vaccine. The nanosubunit vaccine PLGA-rEmLPL is prepared by coating E. maxima Treg-induced molecule EmLPL recombinant protein (rEmLPL) with PLGA nanoparticles. The immunoprotective effects of the subunit vaccine rEmLPL and the nanosubunit vaccine PLGA-rEmLPL are evaluated through animal immunoprotection tests, and the results show that both the vaccines can produce good immunoprotective effects on E. maxima infection. After the recombinant protein subunit vaccine rEmLPL is coated with the PLGA nanoparticles, the immunoprotective effect of the nanosubunit vaccine PLGA-rEmLPL on animals is improved compared with that of the subunit vaccine rEmLPL.

The invention discloses a Cas enzyme mutant, a combined protein, a nucleic acid molecule, a recombinant vector, a transgenic cell and application of the Cas enzyme mutant and the combined protein, the nucleic acid molecule, the recombinant vector, the transgenic cell and the application of the Cas enzyme mutant and the combined protein, and relates to the technical field of gene editing. According to the Cas enzyme mutant, PAM recognition preference (close to TTNPAM) of a Cas12a family is reserved, the editing efficiency is remarkably improved, the highest editing efficiency reaches 100% and is improved by 1.2-3.4 times compared with that of a wild type, a Cas protein optimization strategy of point mutation, structural domain replacement and combined modification is provided, a new path is provided for modification of low-activity Cas protein, the mutant shows efficient editing activity in rice, and the Cas enzyme mutant has good application prospects. The method can be widely applied to genetic improvement of crops and has important agricultural application value.

The invention discloses a lard liquid modification method based on enzymatic transesterification, and relates to the field of biochemistry, a solvent-free enzymatic transesterification reaction is adopted, lard and selected vegetable oil are mixed according to a preset mass ratio, and triacylglycerol molecular recombination is realized through a commercial lipase catalytic transesterification reaction. The modified lard oil is applied to food processing as a food base material or as a matrix of essence, perfume and lipophilic active molecules. The production process is simplified, the yield and stability of the liquid lard oil are improved, the problem that the lard oil is poor in flavor is solved, and the application market of the lard oil is widened.

The invention provides an mRNA molecule of IFN-omega, a recombinant expression vector, lipid nanoparticles and application, the mRNA molecule of IFN-omega comprises an ORF sequence for coding IFN-omega protein, and the nucleotide sequence of the ORF sequence is as shown in SEQ ID NO: 7. The invention develops a novel antiviral treatment scheme for expressing IFN-omega in vivo by using mRNA-LNP, so that the treatment effect of resisting FCV and FHV-1 is improved, the bioavailability is improved, the safety risk is reduced, and the application has important clinical significance and application value.

The application belongs to the technical field of bioengineering, and relates to a marine-derived antibacterial peptide FP2606 and a use of the marine-derived antibacterial peptide FP2606 in preparation of a skin antibacterial drug. Streptomyces globisporus The application also provides a DNA molecule encoding the antibacterial peptide, a recombinant expression vector and an engineering strain, and realizes high-efficiency heterologous expression of the FP2606 in vitro. Experimental verification shows that the antibacterial peptide FP2606 has strong bacteriostatic activity on common pathogenic bacteria causing skin infection of children, has extremely high biological safety, has no hemolytic activity, no skin cell toxicity and no skin irritation, perfectly adapts to the physiological characteristics of the tender skin of children, and can be widely applied to preparation of a skin external antibacterial preparation for children, a skin infection prevention and treatment drug for children and a skin barrier care product for children.

The invention discloses a fusion protein CTB-3MOG34-56, a DNA (deoxyribonucleic acid) molecule, a recombinant vector and application of the fusion protein CTB-3MOG34-56. Belongs to the technical field of biological medicine. The CTB-3MOG34-56 fusion protein is constructed for the first time, and it is proved that the CTB-3MOG34-56 fusion protein can significantly inhibit disease progression of EAE mice through oral administration or nasal mucosa administration, reduce inflammatory infiltration of a central nervous system and significantly inhibit Th17-mediated inflammation (IL-17A and IL-6) and TNF-alpha pathways at the same time; and the TGF-beta is specifically up-regulated to enhance the immunosuppression function of Treg. The fusion protein has the effects of prophylactic immunoregulation and therapeutic neuroprotection, provides a new potential candidate drug for the treatment of experimental autoimmune encephalomyelitis and multiple sclerosis, and has a wide clinical application prospect.

The present disclosure relates to nucleic acid molecules (e.g., self-replicating RNAs (srRNAs) expressing one or more antigenic determinants derived from Epstein-Barr virus (EBV)), recombinant cells containing the nucleic acid molecules and pharmaceutical compositions, and uses of such srRNA molecules, recombinant cells and compositions for eliciting pharmacodynamic effects in a subject. Methods for preventing and / or treating a variety of health conditions associated with EBV infection are also provided.

The application relates to the field of enzyme engineering, in particular to a xylanase, a xylanase optimization design method, a DNA molecule, a recombinant plasmid and a modified cell. The xylanase with an amino acid sequence of SEQ ID NO: 2 has high enzyme activity and thermal stability. The sequence of the xylanase is obtained through optimization design. The mechanism of a multi-domain matrix enzyme is predicted through an online protein structure prediction platform, and the motion trajectory of the matrix enzyme in a solution is simulated under an AMBER99SB force field through molecular dynamics simulation software, the root mean square fluctuation values of different fragments in a connecting section between adjacent functional domains of the matrix enzyme are analyzed and calculated, the fragment with the maximum root mean square fluctuation value in the connecting section is deleted, and the amino acid sequence of the design enzyme is obtained. The optimization design of the enzyme through the method has high reliability and is helpful to improve the enzyme activity and thermal stability of the matrix enzyme.

The invention discloses a method for detecting molecular weight of small-molecular recombinant collagen peptide. According to the method, four commercial peptides are adopted as standard substances, the same high performance liquid chromatography detection method is adopted to respectively carry out high performance liquid chromatography detection on the standard substances and the small molecule recombinant collagen peptides of a test sample, and the obtained atlas of the test sample and a standard curve constructed by the standard substances are compared, calculated and analyzed. Therefore, the molecular weight of the test sample is determined. The method disclosed by the invention can be used for effectively identifying and analyzing the small-molecule recombinant collagen peptide produced by a heat treatment process, and meets the requirements of daily quality detection of the small-molecule recombinant collagen peptide.

The invention discloses a feed additive for antibiotic-free breeding as well as a preparation method and application of the feed additive. The additive takes dual-vector co-expression recombinant lactococcus lactis engineering bacteria as a core and is supplemented by microencapsulated functional small molecules. The recombinant engineering bacterium can synchronously express the fusion polypeptide and a single-chain antibody fragment, wherein the fusion polypeptide contains an N-terminal antibacterial functional domain, a flexible linker and a C-terminal immune enhancement functional domain, and antibacterial-immune synergy is realized; the single-chain antibody fragment aims at escherichia coli F18 adhesin and can specifically block pathogenic bacterium colonization. During preparation, the recombinant engineering bacteria are firstly prepared into freeze-dried powder, and then the freeze-dried powder is compounded with the microencapsulated functional micromolecules in proportion. Experiments show that the additive can remarkably improve the growth performance of weaned piglets, reduce the diarrhea rate, enhance the immune function and improve intestinal health, the effect of the additive is better than that of traditional antibiotics and a scheme of directly adding functional components, and efficient, safe and sustainable technical support is provided for antibiotic-free breeding.

The invention provides a hair disulfide bond repairing composition and application thereof, and relates to the technical field of cosmetics, the hair disulfide bond repairing composition comprises ginsenoside CK, a recombinant collagen composition and multiple hydrolyzed protein, the recombinant collagen composition comprises small-molecule recombinant collagen, middle-molecule recombinant collagen and large-molecule recombinant collagen, and the multiple hydrolyzed protein comprises hydrolyzed vegetable protein, hydrolyzed sericin, hydrolyzed wheat protein, hydrolyzed soybean protein and hydrolyzed corn protein. The ginsenoside CK, the recombinant collagen composition and the multi-hydrolyzed protein are combined for use, so that the antioxidant capacity of the composition reaches the highest level, active oxygen can be removed to the greatest extent to prevent new fracture of disulfide bonds, the capacity of repairing the disulfide bonds can reach the highest, and the effect of repairing the disulfide bonds is achieved. The problem that hair is easy to break due to factors such as dyeing, perming and high temperature is fundamentally solved.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BccFae3074, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BcFae4711, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The application discloses a Cas enzyme mutant and a combined protein, a nucleic acid molecule, a recombinant vector, a transgenic cell and application thereof, relates to the field of gene editing technology, and the mutant is obtained based on CasEDG-01 protein modification, and the amino acid sequence of the CasEDG-01 protein is shown as SEQ ID NO. 1. The Cas enzyme mutant retains the PAM recognition preference (close to TTN PAM) of the Cas12a family, the editing efficiency is significantly improved, is up to 100%, is 1.2-3.4 times higher than that of the wild type, and an optimization strategy of point mutation, domain replacement and combined modification of the Cas protein is provided, a new path for the modification of low-activity Cas protein is provided, and the mutant shows high editing activity in rice, can be widely applied to crop genetic improvement, and has important agricultural application value.

The present invention relates to a system and method for manipulating water-based super vapor or gas to form charged ions. The system comprises a tube chamber (1) that houses the magnetrons (2), a power supply and control system (3) to regulate the emission of the magnetic field, a gas input system to introduce additional gases, and an output system to direct the processed vapor or gas to the condensation system (4). The method involves obtaining water-based super vapor or gas from a liquid source and subjecting it to an array of electromagnetic sources equipped with magnetrons (2) that generate a magnetic field. This exposure charges and ionizes the vapor or gas. Optionally, one or more additional gases may be introduced during this process to facilitate molecular recombination through restructuring, resulting in stable molecules. These restructured molecules can then be directed to a cooling or condensation system for collection. The present invention provides an efficient approach for enhancing the reactivity and stability of super vapor and gases for various industrial applications, including advanced oxidation processes, energy generation, and environmental remediation.

The application relates to preparation and application of a nano-subunit vaccine for preventing chicken coccidiosis. The nano-subunit vaccine PLGA-rEmTregIM-1 is prepared by coating a giant Eimeria maxima Treg inducing molecule EmTregIM-1 recombinant protein (rEmTregIM-1) with PLGA nanoparticles. The immunoprotective effects of the subunit vaccine rEmTregIM-1 and the nano-subunit vaccine PLGA-rEmTregIM-1 are evaluated through animal immunoprotection tests, and the results show that both the vaccines can produce partial immunoprotections for the giant Eimeria maxima infection. After the recombinant protein subunit vaccine rEmTregIM-1 is coated with the PLGA nanoparticles, the nano-subunit vaccine PLGA-rEmTregIM-1 has an improved immunoprotection effect on animals compared with the subunit vaccine rEmTregIM-1.

The invention discloses application of a Zm00001d033798 gene in regulation and control of the height of a corn plant, and belongs to the technical field of regulation and control of the height of the corn plant. The corn plant height key regulation gene Zm00001d033798 is cloned and functionally verified for the first time, the nucleotide sequence of the corn plant height key regulation gene Zm00001d033798 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO: 2. By inhibiting or knocking out the function of the gene, the plant height of the corn can be stably reduced by about 20-40 cm, the dwarfing effect is derived from uniform shortening between stem sections, and key yield characters such as spike length are not influenced. The invention provides application of a DNA molecule containing the gene, a recombinant vector, an expression cassette and a related gene editing tool (such as specific sgRNA) to reduction of corn plant height and cultivation of dwarf close planting resistant varieties. The gene provides a fully verified efficient target for corn plant type improvement and molecular breeding, and has important application value.

This invention belongs to the field of bioengineering technology and relates to marine-derived antimicrobial peptide FP2664 and its use in preparing skin antimicrobial drugs. The amino acid sequence of the antimicrobial peptide FP2664 is shown in SEQ ID NO.1. This antimicrobial peptide FP2664 is derived from marine bacteria of the genus *Cordia*. Cordia This invention also provides the DNA molecule encoding the antimicrobial peptide, the recombinant expression vector, and the engineered strain, achieving efficient heterologous expression of FP2664 in vitro. Experimental verification shows that the antimicrobial peptide exhibits good stability under the tested conditions, has a stable spatial structure and possesses environmental adaptability unique to marine microorganisms, while also demonstrating highly efficient nucleic acid (DNA / RNA) binding activity and broad-spectrum antimicrobial activity, showing significant industrial application prospects in the fields of medicine, food, and environmental remediation.

The invention provides feruloyl esterase, a DNA molecule for coding the feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the feruloyl esterase in production of the feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. The feruloyl esterase BgFae2148 is subjected to genetic engineering modification to obtain a corresponding expression vector and an engineering bacterium, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The present disclosure relates generally to nucleic acid molecules, e.g., self-replicating RNA (srRNA) expressing glucagon-like peptide 1 receptor (GLP-1R) agonists, as a monogenic or multigenic along with GPCR agonists, GIPR agonists, and GCGR agonists, pharmaceutical compositions and recombinant cells containing the same, as well as the use of such srRNA molecules, recombinant cells, and compositions for activating GLP-1R signaling in a subject. Also provided are methods for preventing and / or treating various health conditions associated with GLP-1R signaling.

The present disclosure relates to nucleic acids molecules, e.g., self-replicating RNA (srRNA) expressing antigenic determinant(s) derived from Epstein-Barr virus (EBV), recombinant cells and pharmaceutical compositions containing the same, as well as the use of such srRNA molecules, recombinant cells, and compositions for eliciting a pharmacodynamic effect in a subject. Also provided are methods for preventing and / or treating various health conditions associated with EBV infection.

This invention belongs to the field of bioengineering technology and relates to marine-derived antimicrobial peptide FP2667 and its use in preparing skin antimicrobial drugs. The amino acid sequence of the antimicrobial peptide FP2667 is shown in SEQ ID NO.1. This antimicrobial peptide FP2667 is derived from marine Streptomyces. Streptomyces sp This invention also provides the DNA molecule encoding the antimicrobial peptide, the recombinant expression vector, and the engineered strain, achieving efficient heterologous expression of FP2667 in vitro. Experimental verification shows that the antimicrobial peptide exhibits good stability under the tested conditions, has a stable spatial structure and possesses environmental adaptability unique to marine microorganisms, while also demonstrating highly efficient nucleic acid (DNA / RNA) binding activity and broad-spectrum antimicrobial activity, showing significant industrial application prospects in the fields of medicine, food, and environmental remediation.