38 results about "Molecular recombination" patented technology

The invention belongs to the technical field of biology, and provides a porcine epidemic diarrhea virus mRNA vaccine and application. The mRNA molecule comprises a coding region for coding the S protein of the PEDV; the PEDV is of a G2a type, and two proline mutations I1076P and L1077P are introduced into an S protein. The invention further discloses a DNA molecule. An S protein of PEDV; a recombinant plasmid; recombining the host cell; an mRNA vaccine formulation which can be used to prepare an antibody against the S protein of PEDV after immunizing an animal; the invention further discloses application of the mRNA molecule, the DNA molecule, the recombinant plasmid or the host cell to preparation of a medicine for preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The vaccine prepared by the invention is fast in antibody generation and high in protection rate, can generate high-level neutralizing antibodies in serum and breast milk of sows after the pregnant sows are immunized before parturition, has a remarkable protection effect on piglets, and can effectively prevent the piglets from being infected with PEDV (Porcine Epidemic Diarrhea Virus).

The invention provides a micromolecular recombinant XVII type collagen as well as a preparation method and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening a plurality of spiral region sequences such as a collagen structural domain, a C-terminal region and a middle region of the human XVII type collagen. The amino acid composition of the recombinant XVII type collagen is consistent with that of a natural collagen alpha1 chain, immunological rejection is not generated when the recombinant XVII type collagen is applied to a human body, the recombinant XVII type collagen can be widely applied to surgical medical treatment and medical beauty industries, the molecular weight of the collagen is small, efficient secretion soluble expression in eukaryotic host cells such as pichia pastoris can be achieved, and the recombinant XVII type collagen has a good application prospect. And industrial large-scale production is carried out. According to the present invention, the verification results show that the micromolecular recombinant XVII type collagen further has basement membrane anti-aging activity, wherein the basement membrane anti-aging activity specifically comprises the improvement of the expression levels of the IV type collagen, the VII type collagen and the XVII type collagen;

Provided are a recombinant collagen, an expression method and use thereof. In the present disclosure, the recombinant collagen is expressed by a human embryonic kidney cell Expi293F at a high protein expression level, thereby realizing the expression of a large-molecular-weight recombinant collagen. Activity studies have showed that the expressed recombinant collagen has desirable activity in promoting cell migration, while the recombinant collagen III+I with a fusion tag shows high stability at 4° C. In addition, cell activity studies have shown that the recombinant type III collagen expressed by human cells has better activity in promoting the cell migration than that of a commercially available type III collagen. These results indicate that the human cell expression system is conducive to the expression of highly active and large-molecular-weight recombinant collagen.

The present disclosure relates to RNA molecules, recombinant nucleic acids and gene therapy vectors for the treatment of diseases and conditions associated with myelination deficiency or dysfunction.

The application relates to preparation and application of a giant Eimeria nanosubunit vaccine. The nanosubunit vaccine PLGA-rEmLPL is prepared by coating E. maxima Treg-induced molecule EmLPL recombinant protein (rEmLPL) with PLGA nanoparticles. The immunoprotective effects of the subunit vaccine rEmLPL and the nanosubunit vaccine PLGA-rEmLPL are evaluated through animal immunoprotection tests, and the results show that both the vaccines can produce good immunoprotective effects on E. maxima infection. After the recombinant protein subunit vaccine rEmLPL is coated with the PLGA nanoparticles, the immunoprotective effect of the nanosubunit vaccine PLGA-rEmLPL on animals is improved compared with that of the subunit vaccine rEmLPL.

The present invention discloses a small-molecule recombinant collagen peptide and a method for preparing the same. The method involves placing an aqueous solution of recombinant collagen with a molecular weight of 55 kD into a reactor and heat-treating the reactor to a temperature of 121-125°C using steam heating and pressure control to produce a small-molecule recombinant collagen peptide with an average molecular weight of 1000 Da and a molecular weight of 4000 Da. While maintaining the good biological activity of the original recombinant collagen, the small-molecule recombinant collagen peptide produced by the present invention exhibits improved transdermal absorption, a short onset of action, and rapid efficacy.

The invention discloses a Cas enzyme mutant, a combined protein, a nucleic acid molecule, a recombinant vector, a transgenic cell and application of the Cas enzyme mutant and the combined protein, the nucleic acid molecule, the recombinant vector, the transgenic cell and the application of the Cas enzyme mutant and the combined protein, and relates to the technical field of gene editing. According to the Cas enzyme mutant, PAM recognition preference (close to TTNPAM) of a Cas12a family is reserved, the editing efficiency is remarkably improved, the highest editing efficiency reaches 100% and is improved by 1.2-3.4 times compared with that of a wild type, a Cas protein optimization strategy of point mutation, structural domain replacement and combined modification is provided, a new path is provided for modification of low-activity Cas protein, the mutant shows efficient editing activity in rice, and the Cas enzyme mutant has good application prospects. The method can be widely applied to genetic improvement of crops and has important agricultural application value.

The invention discloses a lard liquid modification method based on enzymatic transesterification, and relates to the field of biochemistry, a solvent-free enzymatic transesterification reaction is adopted, lard and selected vegetable oil are mixed according to a preset mass ratio, and triacylglycerol molecular recombination is realized through a commercial lipase catalytic transesterification reaction. The modified lard oil is applied to food processing as a food base material or as a matrix of essence, perfume and lipophilic active molecules. The production process is simplified, the yield and stability of the liquid lard oil are improved, the problem that the lard oil is poor in flavor is solved, and the application market of the lard oil is widened.

The invention provides an mRNA molecule of IFN-omega, a recombinant expression vector, lipid nanoparticles and application, the mRNA molecule of IFN-omega comprises an ORF sequence for coding IFN-omega protein, and the nucleotide sequence of the ORF sequence is as shown in SEQ ID NO: 7. The invention develops a novel antiviral treatment scheme for expressing IFN-omega in vivo by using mRNA-LNP, so that the treatment effect of resisting FCV and FHV-1 is improved, the bioavailability is improved, the safety risk is reduced, and the application has important clinical significance and application value.

The application belongs to the technical field of bioengineering, and relates to a marine-derived antibacterial peptide FP2606 and a use of the marine-derived antibacterial peptide FP2606 in preparation of a skin antibacterial drug. Streptomyces globisporus The application also provides a DNA molecule encoding the antibacterial peptide, a recombinant expression vector and an engineering strain, and realizes high-efficiency heterologous expression of the FP2606 in vitro. Experimental verification shows that the antibacterial peptide FP2606 has strong bacteriostatic activity on common pathogenic bacteria causing skin infection of children, has extremely high biological safety, has no hemolytic activity, no skin cell toxicity and no skin irritation, perfectly adapts to the physiological characteristics of the tender skin of children, and can be widely applied to preparation of a skin external antibacterial preparation for children, a skin infection prevention and treatment drug for children and a skin barrier care product for children.

The present invention relates to the field of biofuel technology, and discloses a clean molecular recombinant biofuel and a method for preparing the same. The method comprises five steps: raw material pretreatment and component classification, acoustic wave-enhanced synergistic conversion, in-situ hydrogen supply and activation, phase change thermal energy utilization and byproduct conversion, and bio-interface regulation and heterogeneous catalysis. The present invention achieves diversified raw material sources through the synergistic conversion of waste oils and lignin, employs an ultrasonic field and an interphase catalytic system to reduce reaction conditions, utilizes biomass derivatives as a hydrogen source for efficient hydrogenation, utilizes phase change materials to recover heat energy to form an energy closed loop, and enhances catalyst performance through bio-interface regulation technology, ultimately producing a clean biofuel with stable quality that meets aviation fuel standards.

The invention discloses a fusion protein CTB-3MOG34-56, a DNA (deoxyribonucleic acid) molecule, a recombinant vector and application of the fusion protein CTB-3MOG34-56. Belongs to the technical field of biological medicine. The CTB-3MOG34-56 fusion protein is constructed for the first time, and it is proved that the CTB-3MOG34-56 fusion protein can significantly inhibit disease progression of EAE mice through oral administration or nasal mucosa administration, reduce inflammatory infiltration of a central nervous system and significantly inhibit Th17-mediated inflammation (IL-17A and IL-6) and TNF-alpha pathways at the same time; and the TGF-beta is specifically up-regulated to enhance the immunosuppression function of Treg. The fusion protein has the effects of prophylactic immunoregulation and therapeutic neuroprotection, provides a new potential candidate drug for the treatment of experimental autoimmune encephalomyelitis and multiple sclerosis, and has a wide clinical application prospect.

The invention provides a polymer nano synthetic ice material and a preparation method thereof, and belongs to the technical field of polymer materials. The mechanical property, the wear resistance and the stability of the material are enhanced through the double-layer core-shell structure of the nano silicon dioxide and the silane coupling agent; according to the present invention, with the combination of the synergistic effect of the disulfide bond of cystamine and the Schiff base dynamic bond in the dynamic self-repairing prepolymer, the molecular recombination and the self-repairing after the damage are achieved, and the reaction of the diamine and the isocyanate improves the crosslinking density so as to make the material have excellent toughness, excellent strength and excellent aging resistance; besides, by introducing bis (aminopropyl) polydimethylsiloxane, perfluorooctyl acrylate and trimethylolpropane triacrylate, the surface energy is synergistically reduced, the sliding performance and the anti-fouling performance are enhanced, and meanwhile, the hardness, the wear resistance and the thermal stability are further improved through a three-dimensional network structure with high crosslinking density, so that the material is endowed with excellent comprehensive performance.

The invention discloses mRNA (messenger ribonucleic acid) molecules of horse chorionic gonadotropin, a recombinant expression vector, a lipid nanoparticle preparation and application. Relates to the technical field of therapeutic drugs, the mRNA molecule comprises sequences for coding an eCG protein alpha subunit and a beta subunit, the amino acid sequence of the alpha subunit is shown as SEQ ID NO.5, and the amino acid sequence of the beta subunit is shown as SEQ ID NO.6; the alpha subunit and the beta subunit are expressed by connecting the same mRNA molecule through an IRES sequence or a P2A sequence, or the alpha subunit and the beta subunit are connected through a Linker sequence or a Linker-Fold-Linker sequence or a Linker-GCN4-Linker. The technical scheme of the invention aims to design a novel mRNA molecule capable of improving the content of chorionic gonadotropin.

The invention discloses a process for green synthesis of hydrotalcite through a forced molecular recombination diffusion technology, which comprises the following steps: S1, preparation of a magnesium-aluminum emulsion: in the presence of water, stirring and mixing a magnesium source, an aluminum source and a grinding aid to obtain the magnesium-aluminum emulsion, and grinding the magnesium-aluminum emulsion at low temperature for later use; s2, crystallization reaction: adding water to dilute the magnesium-aluminum emulsion subjected to low-temperature grinding, heating to a first temperature, then carrying out high-temperature grinding and ultrasonic dispersion under a closed condition, simultaneously heating to the first temperature, introducing CO3 < 2-> into the system, and continuously heating to a second temperature for crystallization reaction so as to promote the growth of magnesium-aluminum hydrotalcite crystal grains; s3, modification: cooling the crystallization reaction system to a third temperature, adding a modifier into the system for modification, continuously cooling to a fourth temperature, and filtering or centrifuging to obtain water-containing magnesium-aluminum hydrotalcite; the third temperature is lower than the first temperature; and S4, drying: drying the water-containing magnesium-aluminum hydrotalcite to obtain the magnesium-aluminum hydrotalcite.

The present disclosure relates to nucleic acid molecules (e.g., self-replicating RNAs (srRNAs) expressing one or more antigenic determinants derived from Epstein-Barr virus (EBV)), recombinant cells containing the nucleic acid molecules and pharmaceutical compositions, and uses of such srRNA molecules, recombinant cells and compositions for eliciting pharmacodynamic effects in a subject. Methods for preventing and / or treating a variety of health conditions associated with EBV infection are also provided.

The application relates to the field of enzyme engineering, in particular to a xylanase, a xylanase optimization design method, a DNA molecule, a recombinant plasmid and a modified cell. The xylanase with an amino acid sequence of SEQ ID NO: 2 has high enzyme activity and thermal stability. The sequence of the xylanase is obtained through optimization design. The mechanism of a multi-domain matrix enzyme is predicted through an online protein structure prediction platform, and the motion trajectory of the matrix enzyme in a solution is simulated under an AMBER99SB force field through molecular dynamics simulation software, the root mean square fluctuation values of different fragments in a connecting section between adjacent functional domains of the matrix enzyme are analyzed and calculated, the fragment with the maximum root mean square fluctuation value in the connecting section is deleted, and the amino acid sequence of the design enzyme is obtained. The optimization design of the enzyme through the method has high reliability and is helpful to improve the enzyme activity and thermal stability of the matrix enzyme.

The invention relates to the technical field of biofuels, and discloses a clean type molecular recombination biofuel and a preparation method thereof, and the preparation method comprises the following five steps: raw material pretreatment and component grading, sound wave reinforced synergistic conversion, in-situ hydrogen supply and activation, phase change heat energy utilization and by-product conversion, and biological interface regulation and control and heterogeneous catalysis. According to the invention, raw material source diversification is realized through synergistic conversion of waste grease and lignin, reaction conditions are reduced by adopting an ultrasonic field and an interphase catalytic system, efficient hydrogenation is realized by using a biomass derivative as a hydrogen source, heat energy is recovered by using a phase change material to form an energy closed loop, and catalyst performance is improved through a biological interface regulation technology; and finally, the clean biofuel which is stable in quality and meets the aviation fuel standard is prepared.

The present invention provides an electrochemistry biosensor based on a recombinant human renin molecularly imprinted polymer, a preparation method and an application thereof. The electrochemistry biosensor has a recombinant human renin molecularly imprinted polymer loaded on the surface of an electrode. Among them, the molecularly imprinted polymer is prepared by an electrochemical polymerization method using recombinant human renin as a template and methyldopa as a functional monomer. The electrochemistry biosensor has strong specificity for the target molecule recombinant human renin, can specifically detect the target molecule recombinant human renin, and can also treat hypertension, achieving the purpose of integrating detection and treatment. The preparation method of the electrochemistry biosensor is simple, convenient to operate and has a low cost.

The invention discloses a method for detecting molecular weight of small-molecular recombinant collagen peptide. According to the method, four commercial peptides are adopted as standard substances, the same high performance liquid chromatography detection method is adopted to respectively carry out high performance liquid chromatography detection on the standard substances and the small molecule recombinant collagen peptides of a test sample, and the obtained atlas of the test sample and a standard curve constructed by the standard substances are compared, calculated and analyzed. Therefore, the molecular weight of the test sample is determined. The method disclosed by the invention can be used for effectively identifying and analyzing the small-molecule recombinant collagen peptide produced by a heat treatment process, and meets the requirements of daily quality detection of the small-molecule recombinant collagen peptide.

The invention discloses a feed additive for antibiotic-free breeding as well as a preparation method and application of the feed additive. The additive takes dual-vector co-expression recombinant lactococcus lactis engineering bacteria as a core and is supplemented by microencapsulated functional small molecules. The recombinant engineering bacterium can synchronously express the fusion polypeptide and a single-chain antibody fragment, wherein the fusion polypeptide contains an N-terminal antibacterial functional domain, a flexible linker and a C-terminal immune enhancement functional domain, and antibacterial-immune synergy is realized; the single-chain antibody fragment aims at escherichia coli F18 adhesin and can specifically block pathogenic bacterium colonization. During preparation, the recombinant engineering bacteria are firstly prepared into freeze-dried powder, and then the freeze-dried powder is compounded with the microencapsulated functional micromolecules in proportion. Experiments show that the additive can remarkably improve the growth performance of weaned piglets, reduce the diarrhea rate, enhance the immune function and improve intestinal health, the effect of the additive is better than that of traditional antibiotics and a scheme of directly adding functional components, and efficient, safe and sustainable technical support is provided for antibiotic-free breeding.

The invention provides a hair disulfide bond repairing composition and application thereof, and relates to the technical field of cosmetics, the hair disulfide bond repairing composition comprises ginsenoside CK, a recombinant collagen composition and multiple hydrolyzed protein, the recombinant collagen composition comprises small-molecule recombinant collagen, middle-molecule recombinant collagen and large-molecule recombinant collagen, and the multiple hydrolyzed protein comprises hydrolyzed vegetable protein, hydrolyzed sericin, hydrolyzed wheat protein, hydrolyzed soybean protein and hydrolyzed corn protein. The ginsenoside CK, the recombinant collagen composition and the multi-hydrolyzed protein are combined for use, so that the antioxidant capacity of the composition reaches the highest level, active oxygen can be removed to the greatest extent to prevent new fracture of disulfide bonds, the capacity of repairing the disulfide bonds can reach the highest, and the effect of repairing the disulfide bonds is achieved. The problem that hair is easy to break due to factors such as dyeing, perming and high temperature is fundamentally solved.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BccFae3074, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BcFae4711, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The application discloses a Cas enzyme mutant and a combined protein, a nucleic acid molecule, a recombinant vector, a transgenic cell and application thereof, relates to the field of gene editing technology, and the mutant is obtained based on CasEDG-01 protein modification, and the amino acid sequence of the CasEDG-01 protein is shown as SEQ ID NO. 1. The Cas enzyme mutant retains the PAM recognition preference (close to TTN PAM) of the Cas12a family, the editing efficiency is significantly improved, is up to 100%, is 1.2-3.4 times higher than that of the wild type, and an optimization strategy of point mutation, domain replacement and combined modification of the Cas protein is provided, a new path for the modification of low-activity Cas protein is provided, and the mutant shows high editing activity in rice, can be widely applied to crop genetic improvement, and has important agricultural application value.

The present invention relates to a system and method for manipulating water-based super vapor or gas to form charged ions. The system comprises a tube chamber (1) that houses the magnetrons (2), a power supply and control system (3) to regulate the emission of the magnetic field, a gas input system to introduce additional gases, and an output system to direct the processed vapor or gas to the condensation system (4). The method involves obtaining water-based super vapor or gas from a liquid source and subjecting it to an array of electromagnetic sources equipped with magnetrons (2) that generate a magnetic field. This exposure charges and ionizes the vapor or gas. Optionally, one or more additional gases may be introduced during this process to facilitate molecular recombination through restructuring, resulting in stable molecules. These restructured molecules can then be directed to a cooling or condensation system for collection. The present invention provides an efficient approach for enhancing the reactivity and stability of super vapor and gases for various industrial applications, including advanced oxidation processes, energy generation, and environmental remediation.

The application relates to preparation and application of a nano-subunit vaccine for preventing chicken coccidiosis. The nano-subunit vaccine PLGA-rEmTregIM-1 is prepared by coating a giant Eimeria maxima Treg inducing molecule EmTregIM-1 recombinant protein (rEmTregIM-1) with PLGA nanoparticles. The immunoprotective effects of the subunit vaccine rEmTregIM-1 and the nano-subunit vaccine PLGA-rEmTregIM-1 are evaluated through animal immunoprotection tests, and the results show that both the vaccines can produce partial immunoprotections for the giant Eimeria maxima infection. After the recombinant protein subunit vaccine rEmTregIM-1 is coated with the PLGA nanoparticles, the nano-subunit vaccine PLGA-rEmTregIM-1 has an improved immunoprotection effect on animals compared with the subunit vaccine rEmTregIM-1.

The invention relates to the field of enzyme engineering, in particular to xylanase, an optimal design method of the xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a modified cell. The invention relates to xylanase with an amino acid sequence of SEQ ID NO: 2. The xylanase has relatively high enzymatic activity and thermal stability. The sequence of the xylanase is obtained through optimization design. The method comprises the following steps: performing mechanism prediction on a matrix enzyme of multiple structural domains through an online protein structure prediction platform, simulating a motion trail of the matrix enzyme in a solution under an AMBER99SB force field by molecular dynamics simulation software, and analyzing and calculating to obtain a root mean square fluctuation value of different fragments in a connection segment of the matrix enzyme connected between adjacent functional structural domains; and deleting the segment with the maximum root mean square fluctuation value in the connection segment to obtain the amino acid sequence of the designed enzyme. The method is used for carrying out optimal design on the enzyme, so that the method has relatively high reliability and is beneficial to improving the enzyme activity and the thermal stability of the matrix enzyme.

The invention discloses application of a Zm00001d033798 gene in regulation and control of the height of a corn plant, and belongs to the technical field of regulation and control of the height of the corn plant. The corn plant height key regulation gene Zm00001d033798 is cloned and functionally verified for the first time, the nucleotide sequence of the corn plant height key regulation gene Zm00001d033798 is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO: 2. By inhibiting or knocking out the function of the gene, the plant height of the corn can be stably reduced by about 20-40 cm, the dwarfing effect is derived from uniform shortening between stem sections, and key yield characters such as spike length are not influenced. The invention provides application of a DNA molecule containing the gene, a recombinant vector, an expression cassette and a related gene editing tool (such as specific sgRNA) to reduction of corn plant height and cultivation of dwarf close planting resistant varieties. The gene provides a fully verified efficient target for corn plant type improvement and molecular breeding, and has important application value.

This invention belongs to the field of bioengineering technology and relates to marine-derived antimicrobial peptide FP2664 and its use in preparing skin antimicrobial drugs. The amino acid sequence of the antimicrobial peptide FP2664 is shown in SEQ ID NO.1. This antimicrobial peptide FP2664 is derived from marine bacteria of the genus *Cordia*. Cordia This invention also provides the DNA molecule encoding the antimicrobial peptide, the recombinant expression vector, and the engineered strain, achieving efficient heterologous expression of FP2664 in vitro. Experimental verification shows that the antimicrobial peptide exhibits good stability under the tested conditions, has a stable spatial structure and possesses environmental adaptability unique to marine microorganisms, while also demonstrating highly efficient nucleic acid (DNA / RNA) binding activity and broad-spectrum antimicrobial activity, showing significant industrial application prospects in the fields of medicine, food, and environmental remediation.