III-type bacteriocin from L. crispatus, and preparation method and applications thereof
A technology of Lactobacillus crispatus and bacteriocin, which is applied in the biological field, can solve the problem of little research on type III bacteriocin, and achieve the effects of large application value, short production cycle and increased yield
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[0031] The present invention will be described in further detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.
[0032] It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.
[0033] The present invention provides a type III bacteriocin Helveticin-M molecule derived from Lactobacillus crispatus (L.crispatus), the Helveticin-M molecule has antibacterial effect, and the Helveticin-M molecule is as follows (a) or (b) or the polypeptide of (c):
[0034] (a) It is encoded by a DNA molecule whose base sequence is shown in SEQ ID NO:1,
[0035] (b) its amino acid sequence is shown in SEQ ID NO: 2,
[0036] (c) A polypeptide derived from (b) in which the amino acid sequence in (b) has been substituted, deleted or added with one or several amino acids and has...
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