Chemical luminescence immune assay determination reagent kit for detecting human growth hormone

A technology of chemiluminescence immunity and human growth hormone, applied in the field of immunoassay, can solve the problems of radioactive pollution in the environment, short reagent storage time, complicated operation, etc., and achieve the effects of accurate and sensitive results, no radioactive pollution, and fast reaction speed.

Inactive Publication Date: 2009-02-18
北京科美东雅生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Because radioimmunoassay technology uses radioactive elements as markers, it has radioactive pollution to the environment, and has disadvantages such as low sensitivity, complicated operation, and short storage time of reagents; enzyme immunoassay has low sensitivity and low signal-to-noise ratio. Methodological constraints such as difficulty in judging near the gray area; chemiluminescence immunoassay is a more advanced and effective method, which can make the detection sensitivity reach 10 -18 Molar level, its enzyme label is stable and can be used for a long time

Method used

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  • Chemical luminescence immune assay determination reagent kit for detecting human growth hormone
  • Chemical luminescence immune assay determination reagent kit for detecting human growth hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of Growth Hormone Chemiluminescent Immunoassay Assay Kit Alkaline Phosphatase Luminescent Substrate Solution

[0048] Alkaline phosphatase chemiluminescence substrate solution formula:

[0049] Tris (Tris) 6.06g

[0050] 0.1mol / L hydrochloric acid (HCl) 3.45ml

[0051] 4-Methoxy-4-(3-phosphoryloxybenzene)-helix-(1,2-dioxetane-3,2′-adamantane) 150ml

[0052] Magnesium Chloride (MgCl 2 ) 0.15g

[0053] Sodium chloride (NaCl) 9g

[0054] Double distilled water to volume 1000ml

[0055] After the preparation is completed, it is clear and free of turbidity by visual inspection, and it is packed in brown-black polyethylene plastic bottles, each bottle is 10ml.

Embodiment 2

[0056] Example 2 Preparation of Growth Hormone Chemiluminescent Immunoassay Assay Kit Horseradish Peroxidase Luminescent Substrate Solution

[0057] According to the formula of horseradish peroxidase chemiluminescent substrate solution A:

[0058] Boric acid 11.4g

[0059] Borax 4.9g

[0060] Luminol 1.7716g

[0061] 4-Hydroxybiphenyl 0.051g g

[0062] 4-iodophenylboronic acid 0.012g

[0063] Double distilled water to volume 1000ml

[0064] After the preparation is completed, use polyethylene plastic bottles, and each bottle is divided into 10ml.

[0065] Formula of chemiluminescent substrate solution B solution:

[0066] Sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 O) 8.74g

[0067] Disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 51.58g

[0068] Carbamide peroxide (CH 6 N 2 o 3 ) 0.329g

[0069] Tween20 0.1ml

[0070] Double distilled water to volume 1000ml

[0071] After the preparation is completed, brown-black polyethylene plastic bottles are used, and...

Embodiment 3

[0072] Example 3 Preparation of Calibrator of Growth Hormone Chemiluminescent Immunoassay Assay Kit

[0073] Matrix formulation of calibrator according to the invention:

[0074] Horse Serum 300ml

[0075] Goat Serum 100ml

[0076] Casein 1g

[0077] Proclin300 1ml

[0078] Double distilled water to volume 1000ml

[0079] After preparing the matrix solution, dilute the pure growth hormone antigen with reference to the national standard product to a concentration of 5 μIU / ml, 10 μIU / ml, 25 μIU / ml, 50 μIU / ml, and 100 μIU / ml. After the preparation is completed, use a 3ml volume vial, Each bottle was divided into 1ml, and after labeling, it was stored in a freezer at -20°C.

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Abstract

The invention relates to the immunoassay field, in particular discloses a chemiluminescence immunoassay test kit and a preparing method thereof for detecting human growth hormone. The test kit uses principle of enzyme-catalyzed chemiluminescence, and adopts the micro hole plate as the solid-phase carrier so as to achieve batch detection. The monoclonal antibody can be labeled by alkaline phosphatase and horseradish peroxidase. Without radioactive pollution, the test kit has stable performance, simple operation, accurate and sensitive result and swift reaction speed.

Description

technical field [0001] The invention relates to the field of immunoanalysis, in particular, the invention discloses a chemiluminescence immunoassay assay kit for detecting human growth hormone and a preparation method thereof. Background technique [0002] Human Growth Hormone (hGH) is secreted in the anterior pituitary gland. It is a polypeptide containing disulfide bonds in two molecular chains. It exists alone or combined with some growth hormone binding proteins. The main form of growth hormone contains 191 amino acids and has a molecular weight of approximately 22,000. The main biological activity of growth hormone is to directly promote growth. It exerts its biological function by directly promoting bone and muscle growth and indirectly promoting the release of somatomodulin (a group of insulin-like growth factors, insulin-like growth factor, IGF) in the liver. neurological effects, especially somatoregulin C (IGF-1) is necessary for bone growth in children. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/577G01N21/76G01N33/535
Inventor 王宏锐应希堂宋胜利胡国茂唐宝军于尚永
Owner 北京科美东雅生物技术有限公司
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