The invention belongs to the technical field of nano silver antibacterial glue preparation, and discloses nano silver antibacterial glue as well as a preparation method thereof and an intelligent detection system. The intelligent detection system of the nano silver antibacterial glue and the preparation method thereof comprises a weighing module, a time module, a pH detection module, a temperaturedetection module, a main control module, a dissolution module, a mixing and stirring module, a heating module, a detection data storage module and a detection display module. The nano silver antibacterial glue provided by the invention has a better antibacterial effect and is lower in toxicity to human cells; silver in the antibacterial glue provided by the invention is in an atomic state and ata nanometer size, therefore, the loss of silver can be avoided, the oxidation can be delayed, and the antibacterial glue can keep the antibacterial activity for a long time; the invention provides thepreparation of nano silver powder by adopting water as a reaction solvent and an alcohol as a reducing agent, the reaction system is green and environmentally-friendly, high-quality nano silver powder is obtained, and the quality of the nano silver antibacterial glue is improved.