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Preparation method of high-purity lycium polysaccharides

A high-purity, wolfberry technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of increasing production costs, activity and quality degradation, and achieve the effect of ensuring purity and quality.

Pending Publication Date: 2019-07-30
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of Lycium barbarum polysaccharides obtained by this method is high, but soaking and extracting for 8 to 18 hours increases the production cost. The process of microwave and high temperature enzyme inactivation may cause the spatial structure and changes of Lycium barbarum glycopeptides, resulting in a decrease in activity and quality, and decolorization by activated carbon. Will absorb a large amount of Lycium barbarum glycopeptide active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Wash 500g wolfberry fruit with tap water and pure water successively, dry, crush, and pass through a 40-mesh sieve;

[0023] (2) Put the crushed and sieved Lycium barbarum into the extraction bag and tie the mouth, put it into the airtight extraction tank of the subcritical extraction equipment, close the feeding channel, vacuumize to -0.075MPa, and fill it with nitrogen for 10~20min. Pump 0.75L of butane according to the ratio of solid to liquid 1:1.5kg / L, heat to 35°C, set the extraction pressure to 0.5MPa, and the ultrasonic frequency to 15KHz for extraction, the extraction time is 15min, and the extraction is performed 3 times; after the extraction is completed, the The extraction solvent is pumped into the separation tank. Turn on the vacuum pump, reduce the pressure of the separation tank to -0.06MPa, keep the temperature at 35°C, evaporate the butane in the material, and compress the vaporized butane into a liquid state through the diaphragm compressor, retur...

Embodiment 2

[0033] (1) Drying the wolfberry waste residue obtained by preparing 30kg of wolfberry puree, pulverizing, and passing through a 40-mesh sieve;

[0034] (2) Divide the crushed and sieved wolfberry waste residue into extraction bags and tie the mouth. Each bag contains 5 kg, and puts them into 3 closed extraction tanks of subcritical extraction equipment, closes the feeding channel, and evacuates to - 0.075MPa, after filling nitrogen for 10~20min, open the butane storage tank, feed 39L butane according to the ratio of material to liquid 1:1.3kg / L, heat to 35°C, set the extraction pressure to 0.5MPa, the extraction time to 20min, the ultrasonic frequency 15KHz, extraction 5 times, after the extraction, extract the extraction solvent into the separation tank. Turn on the vacuum pump, reduce the pressure of the separation tank to -0.06MPa, keep the temperature at 35°C, evaporate the butane in the material, and compress the vaporized butane into a liquid state through the diaphragm ...

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Abstract

The invention discloses a preparation method of high-purity lycium polysaccharides. The method includes the following steps that lycium barbarums or lycium-barbarum waste residues are crushed, sievedand then subjected to subcritical degreasing, high-speed shearing, low-temperature wall breaking, water extraction, high-speed centrifugation, microfiltration-membrane separation, microfiltration-membrane concentration, inorganic / organic two-phase extraction, impurity removal with macroporous adsorption resin, ethanol precipitation and freeze-drying to obtain the high-purity lycium polysaccharides. It is tested that the active ingredient, namely, lycium barbarum polysaccharide, in the extract is not less than 80%. The lycium polysaccharides are separated by using a 30 kD microfiltration membrane, only the lycium polysaccharides with the molecular weight less than 30 kD are obtained, the lycium polysaccharides with the molecular weight not less than 30 kD are removed, as the lycium polysaccharides with the molecular weight not less than 30 kD are metabolized slowly in vivo, massive accumulation is resulted, 'internal heat' can be induced, and the biological activity of the lycium polysaccharides with the molecular weight not less than 30 kD is low. The four purification technologies are adopted, such as nanofiltration-membrane concentration, two-aqueous-phase extraction, impurity removal with macroporous adsorption resin and ethanol precipitation, and the purity and quality of the obtained lycium polysaccharides are ensured.

Description

technical field [0001] The invention relates to a preparation process of wolfberry glycopeptide, in particular to a preparation process of high-purity wolfberry glycopeptide, and belongs to the technical field of extraction and separation of active ingredients of traditional Chinese medicines. Background technique [0002] Lycium barbarum is a solanaceous plant Lycium barbarum ( Lycium barbarum L. ) dry ripe fruit, its name comes from "Shen Nong's Materia Medica", it has a history of more than 3000 years, and it is the earliest medicinal and edible substance approved by the Ministry of Health. There are many records of the medicinal effects of Lycium barbarum in traditional Chinese medicine classics. Modern pharmacology and natural product chemistry studies have shown that Lycium barbarum glycopeptide (LBP) is one of the main active components of Lycium barbarum. Glycopeptide structure, the content is 3%~9%. Studies have shown that wolfberry glycopeptide is a water-soluble...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/22C07K1/30C07K1/34C07K1/36
CPCC07K1/145C07K1/22C07K1/30C07K1/34C07K1/36
Inventor 邸多隆刘建飞
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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