61results about How to "Positively charged" patented technology

Compositions, systems and related methods for delivering a supercharged protein or a complex of a supercharged protein and therapeutic agent (e g, nucleic acid, peptide, small molecule) to cells are disclosed. Superpositively charged proteins may be associated with nucleic acids (which typically have a net negative charge) via electrostatic interactions. The systems and methods may involve altering the primary sequence of a protein in order to “supercharge” the protein (e g, to generate a superpositively-charged protein). The compositions may be used to treat proliferative diseases, infectious diseases, cardiovascular diseases, inborn errors in metabolism, genetic diseases, etc.

An aspect of the present invention relates to a method of manufacturing a magnetic recording medium comprising dispersing magnetic particles in a water-based solvent having a pH that is lower than an isoelectric point of the magnetic particles to prepare a magnetic liquid, wherein the dispersing is conducted to a state of dispersion where a particle diameter in liquid is equal to or lower than 35 nm; adjusting a zeta potential of the magnetic particles to within a range of 0 to 25 mV by modifying a surface of the magnetic particles with the addition of a prescribed surface-modifying agent to the. magnetic liquid; dispersing the magnetic particles after the adjusting together with an organic solvent and a binder to prepare a magnetic coating material; and forming a magnetic layer of the magnetic recording medium with the magnetic coating material that has been prepared.

The invention provides novel methods for the separation of charged molecules such as proteins according to the isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pI's by shifting both the buffer's pKa and the pI's of the eluted charged molecules while they are traversing the ion exchange column.

An electronic apparatus of the present invention includes an ion generator which generates ions in an atmosphere to remove chemical emission such as VOC and odor. The ion generator is arranged inside the duct which leads the chemical emission generated from the fixing unit to the discharge opening, and also generates positive ions and negative ions in the atmosphere, thereby can efficiently remove the chemical emission. With this arrangement, an electronic apparatus is realized which sufficiently suppresses the chemical emission such as VOC and odor, and is less likely to dirty the surroundings of the electronic apparatus such as the outer surface thereof and the walls surrounding the electronic apparatus.

In a capacitive sensor, a sensing electrode is positively charged, the sensing electrode and a sensing capacitor are charge-balanced, the sensing electrode is negatively charged, and then the sensing electrode and the sensing capacitor are again charge-balanced. The sensing electrode is positively and negatively charged in the same period when the capacitive sensor is sensed. So, the invention can not only effectively improve the high-frequency external interference but also effectively resist the interference when an external direct current electrical field approaches.

Sensors for detecting enzyme activity are provided that include a substrate module comprising a substrate for the enzyme of interest and a fluorescent label, a quencher, and a detection module. The detection module binds to the substrate module either before or after the enzyme acts on the substrate and sequesters the label from the quencher, resulting in an increased signal from the label. Sensors for detecting enzyme activity are also provided that include a substrate for the enzyme, a label, and a quencher that quenches the label. Action of the enzyme on the substrate results in a conformational change that relieves quenching. Sensors for detecting protein-protein interactions are also provided that include a quencher and a labeled first polypeptide. Binding of the first polypeptide to a second polypeptide sequesters the label from the quencher, resulting in an increased signal from the label. Methods using the sensors to detect enzyme activity and to screen for compounds affecting enzyme activity or to detect protein-protein interactions and to screen for compounds affecting protein-protein interactions, respectively, are also described.

The present invention provides a crosslinkable polysiloxane-containing composition and a process for treating a textile with the composition whereby desirable properties, such as water repellency and durability, are enhanced.

The invention relates to an ionic conduction membrane for flow battery and preparation and application thereof. Chloromethylation or bromoethylation organic polymer resin is taken as raw materials toprepare an ionic conduction membrane base body, or the mixed materials of the organic polymer resin and the chloromethylation or the bromoethylation organic polymer resin are taken as raw materials toprepare an ionic conduction membrane base body, a hydrophilic reagent is employed to graft with a hydrophilic group on the ionic conduction membrane base body, the hydrophilic group is a quaternary ammonium group, and a crosslinking agent is employed on the membrane base body to form an ionic conduction membrane with a crosslinked structure, wherein the mass ratio of the organic polymer resin andthe chloromethylation or bromoethylation organic polymer resin is 0:1, and the crosslinking agent is the diamine group. The ionic conduction membrane is simple in process, high in ion transmission performance and good in chemical and mechanical stability so that the all-vanadium redox flow battery assembled by the ionic conduction membrane prepared by employing the method is good in excellent ionic conduction performance, high in ion selection performance and good in cycling stability.

A composition comprising a photoactivatable larvicide and a suitable vector, the latter allowing the larvicide to be ingested by the larvae and a method for controlling mosquito larvae by using said photoactivatable larvicide are disclosed.

Disclosed is a polymer composite (polyplex) that contains nucleic acid, a cationic polymer, and an anionic polymer. The anionic polymer covers the surface of the composite comprising the cationic polymer and nucleic acid, has a negative charge at neutral pH, and can change so as to have a positive charge at mildly acidic pH.

A device for imparting a combination of therapies to a user's body or body parts has a first battery-powered array submerged into a liquid contained in a first reservoir and a second battery-powered array submerged into a liquid contained in a second reservoir. Each array has one or more degradable electrodes that release ions into the liquid in the reservoir. The electrodes can be copper, zinc, steel, silver, nickel, or a combination thereof. The solution in the reservoir may contain positively or negatively charged medicament ions. During a therapy session, the device is operated in one or both of an electrolysis mode and an iontophoresis mode. Direct current is applied to one or more of the electrodes depending on the mode selected and other session parameters. The device uses a current and voltage regulator to deliver a regulated amount of current through the arrays regardless of the conductivity of the liquid, and electronic circuitry is used to control the duration, polarity, electrode choice, and intensity of the treatment. Excessive heat is dissipated with a heat sink.

A wearable iontophoresis device for the prolonged delivery of a positively charged pharmaceutical species from a salt formulation is disclosed that includes a readily oxidizable metal-based sacrificial anode in the form of a generally planar layer having a connecting area that has a width that is sufficient to insure complete consumption of the oxidizable metal wherein the anode is configured to have a minimum operating life of at least 6 hours under skin-safe conditions, and a drug delivery gel pad in electrical contact with said anode for accommodating a gel containing a positively charged pharmaceutical species in salt form formulated for transdermal delivery.

The fuel cell system includes a fuel cell; a power conditioner that converts a cell power supplied from the fuel cell to a power corresponding to a load; a capacitor to which an excessive output power of the fuel cell is charged at the time of a low load operation and from which a power corresponding to the insufficient output power of the fuel cell is discharged at the time of a high load operation; a voltage detection means for detecting a voltage of the capacitor; and a control means that determines the low load operation or the high load operation, calculates an output power from the fuel cell to maintain the excessive power generated at the time of the low load operation within a space capacity of the capacitor, and controls the output power of the fuel cell not to exceed the calculated value.

The invention discloses a novel liquid semiconductor quantum dot fluorescent reagent, the surface of which has positive electricity property, for imprint display and extraction in the criminal investigation field. The liquid semiconductor CdTe/NH3+ quantum dot fluorescent reagent, the surface of which has positive electricity property, is prepared by surface modification of a traditional II-VI semiconductor CdTe quantum dot with a surface modified by mercaptoacetic acid through hydrazine hydrate. The fluorescent reagent solves the key technical problem of mutual repulsion and low affinity of the traditional mercaptoacetic acid modified semiconductor CdTe/COO- quantum dot fluorescent reagent under the alkali condition because the surface charge property is the same as the imprint residue charge property, so the fluorescent reagent has application advantages in the criminal science field. The liquid semiconductor quantum dot fluorescent reagent has the advantages of high fluorescence intensity, simple preparation process and good adhesion with fresh or old imprint, and is suitable for display and extraction of imprints on most impervious objects in criminal investigation.

The invention discloses a preparation method of chitosan/silk fibroin microspheres for thrombin immobilization. The method comprises steps as follows: S1, degummed fibroin fiber is added to an ethanol solution containing calcium chloride to be dissolved, and a fibroin solution is obtained after dialysis and filtration; S2, chitosan is dissolved in an acetic acid solution, the fibroin solution in S1 and a CuSO4 solution are added, and a mixed solution is obtained; S3, the mixed solution in S2 is added to an alkaline ethanol solution, hollow beads are prepared, EDTA is added, complex Cu2<+> is removed through stirring, dehydration, cleaning and drying are performed after filtration, and chitosan/fibroin beads are obtained; S4, chitosan/fibroin beads in S3 are swelled, a thrombin solution and a buffer solution are added, and a glutaraldehyde solution is added for an immobilized enzyme reaction. Chitosan and fibroin are used as carriers for immobilized enzyme, the enzyme stability can be improved, the immobilization activity recovery efficiency is guaranteed, and the method has great actual significance.

The present invention discloses anamine modifying material which is a copolymer polymerized from an amine functional acrylate, a poly(ethylene glycol) functional acrylate and a cross-linker. The application of the amine modifying material in the bio-molecules separation area is also provided in the present invention.

A device for iontophoresis. A first battery-powered array is submerged into a liquid contained in a first reservoir and a second battery-powered array is submerged into a liquid contained in a second reservoir. Each array has one or more degradable electrodes that releases ions into the liquid in the reservoir. The electrodes can be copper, zinc, steel, nickel, or a combination thereof. At the first array one of the electrodes can be positively charged while at the second array one of the electrodes can be negatively charged. Alternatively, an electrode at the second array can be positively charged while an electrode at the first array is negatively charged. The solution in the reservoir may also contain positively or negatively charged ions. Powering the arrays causes the charged molecules contained in the liquid to transport through a patient's skin. The device uses a current and voltage regulator to deliver a regulated amount of current through the arrays regardless of the conductivity of the liquid, and electronic circuitry is used to control the duration, polarity, electrode choice, and intensity of the treatment. Excessive heat is dissipated with a heat sink.

An electrical tool assembly includes a battery-operated electrical tool, which has a housing and a charging terminal mounted in an insertion portion of the housing, and a charger, which has a receptacle fitting the contour of the insertion portion for the insertion of the insertion portion in a predetermined direction, a charging terminal for receiving the charging terminal of the electrical tool to charge the rechargeable battery of the battery-operated electrical tool, and a recessed seat for holding a body of the housing of the electrical tool in position upon insertion of the insertion portion into the receptacle.

An ink jet printer which is a recording device which ejects ink droplets onto a recording surface of a recording medium thereby recording, includes a material of slave rollers that are in contact with the recording surface in a transport path through which the recording medium is transported, the material being located on a polarity side opposite to a polarity in which mist that is generated in accordance with ejection of the ink droplets in a charge string is charged, further than a material of a member which configures the recording surface.