Composite tissue engineering skin containing live cells and preparation method for composite tissue engineering skin

A composite tissue, engineering skin technology, applied in medical science, prosthesis, etc., can solve the problems of harsh growth and storage conditions of epidermal living cells, poor ability to isolate external bacteria, easy to cause infection, etc., to improve the isolation of external bacterial infection. Ability, shorten healing time, overcome the effect of being susceptible to external bacterial infection

Inactive Publication Date: 2015-07-01
陕西艾尔肤组织工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing skin substitutes containing living cells include: a tissue-engineered skin with double-layer living cells, which includes an epidermis made of epidermal living cells and a dermis made of fibroblast living cells. Although this skin substitute is more effective than Good, but due to the harsh grow...

Method used

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  • Composite tissue engineering skin containing live cells and preparation method for composite tissue engineering skin
  • Composite tissue engineering skin containing live cells and preparation method for composite tissue engineering skin
  • Composite tissue engineering skin containing live cells and preparation method for composite tissue engineering skin

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preparation example Construction

[0035] On the other hand, an embodiment of the present invention provides a method for preparing composite tissue-engineered skin containing living cells, including:

[0036]After culturing the cell-collagen gel solution on a polymeric material film, a composite tissue-engineered skin containing living cells is obtained. The composite tissue-engineered skin includes a dermis layer and a degradable polymeric material protective layer, and the dermis layer contains human-derived synthetic materials. Fibroblasts or mesenchymal stem cells that can differentiate into fibroblasts.

[0037] Wherein, the polymer material film can be obtained through commercial channels, and can also be obtained by the following preparation method:

[0038] The polymer material solution or melt is jet-spun under the action of a strong electric field to obtain a superfine fiber membrane.

[0039] The obtained microfiber membrane has the characteristics of small pore size, no pollution, good biocompatib...

Embodiment 1

[0056] 1. Preparation of polylactic-co-glycolic acid (PLGA) film protective layer.

[0057] Specifically include:

[0058] Preparation of high-voltage electrospun non-woven fabric membrane: Jet-spin the polylactic acid-glycolic acid copolymer solution or melt under the action of a strong electric field to obtain an ultrafine fiber PLGA membrane with a pore size less than or equal to 220nm;

[0059] Collagen gel preparation: Dissolve type I collagen in sterile 0.1% acetic acid and stir until dissolved at a low temperature of 6°C;

[0060] Membrane spreading: Submerge the prepared PLGA membrane into the prepared collagen gel, quickly take it out and lay it on the pitot in the skin slice generator, and solidify at 37°C.

[0061] 2. Prepare the cell-collagen gel solution.

[0062] Add 10% newborn bovine serum and 10% 10 times DMEM culture solution to the prepared collagen gel, adjust the pH to 7.0, and mix well-growing skin fibroblasts with neutral collagen gel to obtain fibrobl...

Embodiment 2

[0068] 1. Preparation of polylactic-co-glycolic acid (PLGA) film protective layer.

[0069] Specifically include:

[0070] Preparation of high-voltage electrospun non-woven fabric membrane: Jet-spin the polylactic acid-glycolic acid copolymer solution or melt under the action of a strong electric field to obtain an ultrafine fiber PLGA membrane with a pore size less than or equal to 220nm;

[0071] Collagen gel preparation: Dissolve type I collagen in sterile 0.05% acetic acid and stir until dissolved at a low temperature of 4°C;

[0072] Membrane spreading: Submerge the prepared PLGA membrane into the prepared collagen gel, quickly take it out and lay it in the pitot of the skin slice generator, and solidify at 32°C.

[0073] 2. Prepare the cell-collagen gel solution.

[0074] Add 4% newborn bovine serum and 5% 8 times DMEM culture solution to the prepared collagen gel, adjust the pH to 6.0, and mix well-growing skin fibroblasts with neutral collagen gel to obtain fibroblas...

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Abstract

The invention relates to the biological field, and in particular relates to composite tissue engineering skin containing live cells and a preparation method for the composite tissue engineering skin. The composite tissue engineering skin not only contains live cells, but also contains a degradable polymer material protective layer, so that the composite tissue engineering skin is reinforced in isolating capacity, the cost is reduced and the quality guarantee period is prolonged. The defects that the tissue engineering skin containing live cells in the prior art is high in cost, short in quality guarantee period and poor in isolating capacity are overcome. According to the embodiment of the invention, the composite tissue engineering skin containing live cells provided by the invention comprises a corium layer and a degradable polymer material protective layer, wherein the corium layer comprises liver humanized fibroblast or mesenchymal stem cells which are differentiated towards the fibroblast.

Description

technical field [0001] The invention relates to the field of biology, in particular to a composite tissue engineering skin containing living cells and a preparation method thereof. Background technique [0002] The skin covers the whole body, and it protects various tissues and organs in the body from physical, mechanical, chemical and pathogenic microorganisms. However, with the development of society, there are more and more skin defects caused by various traumas, especially burns, senile diseases, etc., and there are more and more skin substitute repair products applied to skin damage. Among them, tissue engineered skin is Mix dermal fibroblasts with extracellular matrix substitutes to make artificial skin, or simply use porous extracellular matrix instead of dermis to implant wounds, and transplant epithelial cells to cover it or wait for its own epithelial cover to replace the damaged skin. [0003] Existing skin substitutes are of various types, and their effects vary...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/50A61L27/38A61L27/54
Inventor 凡孝菊王海江张爱兵
Owner 陕西艾尔肤组织工程有限公司
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