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Lactobacillus casei Zhang microcapsule, preparation method and applications thereof

A kind of Lactobacillus casei, microcapsule technology, applied in dairy products, fixed on/in organic carrier, application and other directions

Inactive Publication Date: 2010-06-09
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But only a few studies have addressed the use of whey protein

Method used

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  • Lactobacillus casei Zhang microcapsule, preparation method and applications thereof
  • Lactobacillus casei Zhang microcapsule, preparation method and applications thereof
  • Lactobacillus casei Zhang microcapsule, preparation method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0102] Embodiment 1: the preparation method of the microcapsule of Lactobacillus casei Zhang

[0103] This embodiment proceeds according to the following steps:

[0104] a. Bacteria activation:

[0105] Inoculate the cryopreserved Lactobacillus casei Zhang CGMCC 1697 in the MRS liquid medium, culture at 37°C for 18-24 hours, and subculture twice in this way to obtain the activated Lactobacillus casei Zhang strain;

[0106] b. Preparation of culture medium:

[0107] First, weigh the following raw materials respectively: 80 parts by weight of glucose monohydrate, 10 parts by weight of yeast powder, 10 parts by weight of soybean peptone, 3.2 parts by weight of dipotassium hydrogen phosphate, 14 parts by weight of sodium acetate, 2.2 parts by weight of sodium citrate, 0.9 parts by weight parts by weight MgSO 4 .7H 2 O, 0.06 parts by weight MnSO 4 .5H 2 O and 0.9 parts by weight Tween 80;

[0108] Then, the above raw materials were dissolved in 1000 parts by weight of water,...

Embodiment 2

[0118] Example 2: Lactobacillus casei Zhang microcapsules are applied to a probiotic starter.

[0119] Add the Lactobacillus casei freeze-dried bacteria powder into the mixed straight-in starter by Lactobacillus bulgaricus and Streptococcus thermophilus by 10% by weight, mix evenly to obtain the probiotic starter containing Lactobacillus casei (Lactobacillus casei Zhang) . The starter can be used in the production and processing of fermented yoghurt or fermented milk drink.

[0120] Adding the Lactobacillus casei Zhang microcapsules obtained in Example 1 to the starter can increase the prebiotic effect of the probiotic starter. The L.casei Zhang embedded by the above method can maintain a higher survival rate than the unembedded bacteria after being treated with pH2.0 or pH2.5 artificial digestion solution. The method described in this manual was used to measure, and the results showed that the survival rates of the embedded and unembedded Lactobacillus casei Zhang in pH2.0 ...

Embodiment 3

[0122] Example 3: Lactobacillus casei microcapsules are applied to probiotic preparations.

[0123] The Lactobacillus casei microcapsules are added into the bifidobacterium medical probiotic preparation matrix by 10% by weight, mixed evenly, and then made into capsules or tablets, so as to obtain probiotic capsules or probiotic tablets containing Lactobacillus casei.

[0124] Adding the Lactobacillus casei Zhang microcapsules obtained in Example 1 to the probiotic capsules or probiotic tablets can increase the prebiotic effect of the probiotic starter. The L.casei Zhang embedded by the above method can maintain a higher survival rate than the unembedded bacteria after being treated with pH2.0 or pH2.5 artificial digestion solution. The method described in this specification was used to measure, and the results showed that the survival rates of the embedded and unembedded Lactobacillus casei Zhang in pH2.0 and pH2.5 artificial digestion solutions were 58.10%, 66.95%, 34.70%, an...

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Abstract

The invention relates to a microcapsule embedding preparation method of a Lactobacillus casei Zhang microcapsule Zhang CGMCC No.1697, comprising the following steps: activating Lactobacillus casei strain, preparing culture medium, preparing strain fermentation liquor, preparing microcapsule wall material, preparing microcapsule embedding solution and microcapsule.Compared with Lactobacillus casei which is not embedded, the Lactobacillus casei microcapsule prepared by the method of the invention, after being processed in artificial digestive juice, can have viable count increased by one magnitude order.After being preserved for 30 days at 37 DEG C, the Lactobacillus casei microcapsule can have viable count increased by more than one magnitude order.Therefore, the Lactobacillus casei microcapsule prepared by the method of the invention can greatly improve efficacy of probiotics in the process of processing, storage and transportation.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganism protection and application. More specifically, the present invention relates to a preparation method of Lactobacillus casei Zhang microcapsules, and the present invention also relates to the microcapsules prepared by the preparation method and their application in the dairy industry. 【Background technique】 [0002] As we all know, lactic acid bacteria is a general term for a class of Gram-positive bacteria that ferment lactose to produce lactic acid, and are probiotics that have important physiological and health functions for the human body. As a kind of probiotics, lactic acid bacteria have been used in food industry production due to their good fermentation ability. Lactic acid bacteria can not only impart flavor to fermented dairy products, but also prevent many spoilage microorganisms from surviving due to their high acidity, thereby avoiding some gastrointestinal diseases. For lact...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04A23P1/04A23C9/12
Inventor 张和平陈霞
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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