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Applications of lactobacillus casei for antioxidation

A Lactobacillus casei, anti-oxidation technology, applied in biochemical equipment and methods, bacteria, microorganism-based methods, etc., can solve the problem that active oxygen cannot be completely eliminated

Active Publication Date: 2008-12-31
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the active oxygen is constantly produced by the body, the defense system cannot completely eliminate the active oxygen produced by metabolism and stimulation by external conditions.

Method used

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  • Applications of lactobacillus casei for antioxidation
  • Applications of lactobacillus casei for antioxidation
  • Applications of lactobacillus casei for antioxidation

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: a kind of Lactobacillus casei, described Lactobacillus casei (Lactobacillus casei Zhang) bacterial strain is the probiotic bacterial strain of acid resistance and bile acid resistance isolated from kumiss; Committee General Microorganism Collection Center, collection number: CGMCCNo.1697.

[0021] 1. Sample collection and isolation and identification of Lactobacillus

[0022] The sample is: kumiss made by traditional method, and the sample was collected in Zhenglan Banner, Xilingol League, Inner Mongolia, China.

[0023] 2. Isolation and purification of Lactobacillus in samples: see figure 1 , the isolation and purification process of lactobacillus casei (Lactobacillus casei Zhang) among the present invention is as follows:

[0024] Using the traditional functional fermented milk in Inner Mongolia——Kums milk as the sample for the isolation of Lactobacillus, the kos milk produced by the natural fermentation of the herdsman's family is fully stirred in the...

Embodiment 2

[0032] Embodiment 2: an application of Lactobacillus casei as described in Example 1, the application of said Lactobacillus casei in the aspect of antioxidant effect; especially the application of improving the antioxidant effect of fermented milk in fermented milk products.

[0033]The acid resistance of Lactobacillus casei Zhang of the present invention (Lactobacillus casei Zhang) and the tolerance analysis in artificial gastrointestinal fluid thereof:

[0034] Among the 50 strains of Lactobacillus isolated and identified from the kumiss milk sample, after the growth test in the MRS liquid medium with a pH value of 3.5 and the determination of the survival rate in the artificial gastric juice with a pH value of 3.0, one acid-resistant strain was screened. Strong Lactobacillus, Lactobacillus casei Zhang.

[0035] The Lactobacillus casei Zhang strain was tested for growth in MRS liquid medium with a pH value of 3.5 and the survival rate in artificial gastric juice with a pH va...

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Abstract

The invention relates to the preparation of a Lactobacillus casei Zhang strain and an application of the strain at the aspect of antioxidant products. The Lactobacillus casei Zhang strain is an acid- and bile acid-resistant probiotics strain separated from Lactobacillus casei Zhang of fermented mare milk which is the traditional dairy product of the inner Mongolia nationality; and the strain has been stored in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the Collection No. is: CGMCC No.1697. Besides the beneficial function to body health possessed by common lactobacillus, the Lactobacillus casei Zhang of the invention can also be used for the production of anti-oxidation functional products, such as: the production of various dairy products with antioxidant activity; and the Lactobacillus casei Zhang can be present in specific products by mixing with other materials, and can also be produced in the form of live bacteria preparation.

Description

technical field [0001] The invention relates to the preparation of a Lactobacillus casei Zhang strain isolated from the traditional dairy product of the Inner Mongolian nation - kumiss and the application of the strain in anti-oxidation products. technical background [0002] Oxidation is necessary for many living organisms. The energy it produces is used to maintain normal life activities. However, along with the oxidation, it will also produce some reactive oxygen species that can damage biological molecules. These reactive oxygen species include not only Oxygen-centered free radicals, such as O 2 - and OH, including some non-radical derivatives of oxygen, such as H 2 o 2 These reactive oxygen species are closely related to the aging of organisms and the occurrence of diseases. Free radicals in the body can trigger the peroxidation reaction of unsaturated fatty acids in biomembranes to produce products such as lipid peroxide and malondialdehyde (Maleic dialaldehyde, MD...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/245
Inventor 张和平王俊国孟和毕力格
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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