Vibrio parahaemolyticus standard substance containing fish substrate

Abstract

The invention belongs to the technical field of food microorganisms, and particularly relates to a vibrio parahaemolyticus standard substance containing fish substrates. Concretely, a finished productwith the bacterium number being 100 to 350 CFU (colony forming unit) per bottle is prepared through storage liquid preparation, substrate pretreatment, suspension preparation, standard substance preparation, strain identification, uniformity verification, constant value obtaining and stability inspection. The obtained vibrio parahaemolyticus standard substance containing fish substrates has gooduniformity; the problem that the fish substrates in the existing standard substance are difficult to dissolve in water can be solved; the quality is stable; the quality is controllable; the vibrio parahaemolyticus standard substance is applicable to efficient and accurate quantitative detection of microorganisms.

Description

technical field [0001] The invention belongs to the technical field of food microbes, and in particular relates to a standard substance of vibrio parahaemolyticus containing a fish meat matrix. Background technique [0002] Bacteria reference materials are a special type of biological characteristic reference materials. The use of bacterial reference materials can effectively ensure the efficient and accurate operation of microbial quantitative detection. However, at present, only a few bureaus in the entry-exit inspection and quarantine system in China Research and development, most of the reference materials are imported from abroad. In 2006, the standard material for the total number of microbial colonies in food developed by the Liaoning Entry-Exit Inspection and Quarantine Bureau was approved as a national secondary standard material. The standard material includes two development methods: one is made by simulating food, adding Enterobacter, Serratia, Bacillus and othe...

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Problems solved by technology

[0002] Bacteria reference materials are a special type of biological characteristic reference materials. The use of bacterial reference materials can effectively ensure the efficient and accurate operation of microbial quantitative detection. However, at present, only a few bureaus in the entry-exit inspection and quarantine system in China Research and development, most of the reference materials are imported from abroad

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