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Polynucleotides and methods for making plants resistant to fungal pathogens

a technology of fungal pathogens and polynucleotides, which is applied in the field of polynucleotides and methods for making plants resistant to fungal pathogens, can solve the problems of increasing problems, affecting the harvesting of lodged plants, and affecting the quality of the finished produ

Inactive Publication Date: 2006-10-05
UNIVERSITY OF DELAWARE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0025] Further embodiments include corn plants obtainable by a method comprising: crossing MP305 or DE811ASR(BC5) [Deposit No. PTO-7434] as a first parent plant, with a different plant that lacks an Rcg1 locus as a second parent plant, thereby to obtain progeny comprising the Rcg1 locus of the first parent; and optionally further comprising one or more further breeding steps to obtain progeny of one or more further generations comprising the Rcg1 locu

Problems solved by technology

Lodged plants are more difficult to harvest and are susceptible to other diseases.
Roots may grow through stalk debris and become infected.
This will become an increasing problem as “no till” methods of agriculture are more widely adopted due to their environmental benefits.
Farmers may combat infection by corn fungal diseases such as anthracnose through the use of fungicides, but these have environmental side effects, and require monitoring of fields and diagnostic techniques to determine which fungus is causing the infection so that the correct fungicide can be used.
Particularly with large field crops such as corn, this is difficult.
Introgression of resistance derived from such lines is complex.

Method used

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  • Polynucleotides and methods for making plants resistant to fungal pathogens
  • Polynucleotides and methods for making plants resistant to fungal pathogens
  • Polynucleotides and methods for making plants resistant to fungal pathogens

Examples

Experimental program
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Effect test

example 1

Fine Mapping of the Rcg1 Locus to a Specific Region of 4L

[0185] In order to map and clone the gene responsible for the resistance of corn line MP305 to Cg, lines had previously been created which differed as little as possible from each other genetically with the exception of the presence of the locus responsible for the resistant phenotype. Such lines are called near isogenic lines. To this end, DE811 had been crossed to MP305 and the progeny had been backcrossed to the sensitive line DE811 three times, at each backcross selecting for resistance to Cg and otherwise for characteristics of DE811 (Weldekidan and Hawk, (1993), Maydica, 38:189-192). The resulting line was designated DE811ASR (BC3) (Weldekidan and Hawk, (1993) supra). This line was used as the starting point for the fine mapping of the Rcg1 locus. It was first necessary to know roughly where in the maize genome it was located. Using standard genetic methods, Jung et al. ((1994) supra) had previously localized the locus ...

example 2

Isolation of BAC Clones from the Resistant Lines and Identification of Candidate Genes in the Region of the Rcg1 Locus

[0193] In order to isolate the gene responsible for the phenotype conferred by the Rcg1 locus, BACs containing the region between the FLP 8 and FLP 27 markers were isolated from a BAC library prepared from the resistant line DE811ASR (BC5). This library was prepared using standard techniques for the preparation of genomic DNA (Zhang et al. (1995) Plant Journal 7:175-184) followed by partial digestion with HindIII and ligation of size selected fragments into a modified form of the commercially available vector pCC1 BAC™ (Epicentre, Madison, USA). After transformation into EPI300™ E. coli cells following the vendors instructions (Epicentre, Madison, USA), 125,184 recombinant clones were arrayed into 326 384-well microtiter dishes. These clones were then gridded onto nylon filters (Hybond N+, Amersham Biosciences, Piscataway, USA).

[0194] The library was probed with ov...

example 3

Comparison of Genetic Structure in the Region of the Rcg1 Locus Between Resistant and Susceptible Lines and Expression Profiles of Candidate Genes Found in that Region Between Resistant and Susceptible Lines

[0201] Having found a candidate gene in the region genetically defined to carry the locus responsible for the resistance to anthracnose phenotype, efforts were undertaken first to determine if there might be other genes present in the region and second to determine if the expression patterns of the candidate gene were consistent with its putative role. Fu and Dooner ((2002), Proc Natl Acad Sci 99:9573-9578) and Brunner et al. ((2005), Plant Cell 17:343-360) have demonstrated that different corn inbred lines may have significant rearrangements and lack of colinearity with respect to each other. Comparison of such genomes over larger regions can thus be complex. Such a comparison of the genomes of Mo17 (Missouri 17) and DE811ASR (BC5) revealed that in the region where the candidat...

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Abstract

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application claims priority to and the benefit of U.S. Provisional Application Nos. 60 / 668,241 and 60 / 675,664, filed on Apr. 4, 2005 and Apr. 28, 2005, respectively, which are herein incorporated by reference in their entirety.THE NAMES OF THE PARTIES TO A JOINT RESEARCH AGREEMENT [0002] A joint Research Project Agreement was executed on Feb. 18, 2002 for map-based cloning and gene expression studies of a maize gene(s) that confer(s) resistance to ASR. The names of the parties executing the joint Research Project Agreement are the University of Delaware and E.I. du Pont de Nemours and Company. FIELD OF THE INVENTION [0003] This invention relates to compositions and methods useful in creating or enhancing pathogen-resistance in plants. Additionally, the invention relates to plants that have been genetically transformed with the compositions of the invention. BACKGROUND OF THE INVENTION [0004]Colletotrichum graminicola (Ces.) (Cg), m...

Claims

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Application Information

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IPC IPC(8): A01H1/00C07H21/04C12N15/82A01H5/00C12N5/04A01H5/10
CPCA01H1/04A01H5/10C12Q1/6895C12N15/8282C07K14/415C12Q2600/13C12Q2600/156C12Q2600/172A01H6/4684A01H1/045
Inventor BROGLIE, KARENBUTLER, KARLENEDE SILVA CONCEICAO, ALEXANDREFREY, TRAVISHAWK, JAMESMULTANI, DILBAGCECILIA WOLTERS, PETRA
Owner UNIVERSITY OF DELAWARE
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