Disease resistance intensity identifying method for resistance to tobacco anthracnose

An anthracnose resistance and identification method technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of destroying tobacco seedlings, poor seedling vigor, affecting the economic benefits of tobacco planting and the like

Inactive Publication Date: 2017-02-01
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The leaves of seedlings are densely covered with diseased spots, and when the disease is severe, the whole tobacco seedlings are often destroyed. Generally, although the seedlings are not destroyed when the disease occurs, the see

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method for identifying the strength and weakness of tobacco resistance to anthracnose comprises the following steps:

[0021] Step 1, planting tobacco varieties to be identified, known anthracnose-resistant varieties, moderate anthracnose-resistant varieties and known anthracnose-susceptible varieties in the tobacco field;

[0022] Step 2: Transplant indoors, inoculate the anthracnose spores when the tobacco seedlings grow 5 true leaves, spray the spore suspension onto the plants with a small hand-held sprayer, so that the front and back of the leaves are fully moist, and keep the temperature at 26°C after inoculation , growing under environmental conditions with a relative humidity of more than 80%, and inducing morbidity; wherein, the anthrax spore suspension adopts potato dextrose agar (PDA) medium, and is cultivated at 26°C for 6 days, and the total number of bacterial communities contained in each milliliter of anthrax spore suspension 0.8×10 8 CFU / mL;

[002...

Embodiment 2

[0025] The method for identifying the strength and weakness of tobacco resistance to anthracnose comprises the following steps:

[0026] Step 1: Plant tobacco varieties to be identified, known anthracnose-resistant varieties, moderately anthracnose-resistant varieties, known anthracnose-susceptible varieties, local anthracnose-resistant varieties, local anthracnose-resistant varieties and local anthracnose-susceptible varieties in the tobacco field ;

[0027] Step 2: Transplant indoors, inoculate the anthracnose spores when the tobacco seedlings grow 6 true leaves, spray the spore suspension onto the plants with a small hand-held sprayer to make the front and back of the leaves fully moist, and keep the temperature at 30°C after inoculation , grow under environmental conditions with a relative humidity of 80%, and induce disease; wherein, the anthrax spore suspension adopts potato dextrose agar (PDA) medium, and is cultivated at 26°C for 6-8d, and the bacteria contained in eac...

Embodiment 3

[0030] The method for identifying the strength and weakness of tobacco resistance to anthracnose comprises the following steps:

[0031] Step 1, planting tobacco varieties to be identified, known anthracnose-resistant varieties, moderate anthracnose-resistant varieties and known anthracnose-susceptible varieties in the tobacco field;

[0032] Step 2: Transplant indoors, inoculate the anthracnose spores when the tobacco seedlings grow 5 true leaves, spray the spore suspension onto the plants with a small hand-held sprayer to make the front and back of the leaves fully moist, and keep the temperature at 28°C after inoculation , growing under environmental conditions with a relative humidity of more than 80%, and inducing morbidity; wherein, the anthracis spore suspension adopts potato dextrose agar (PDA) medium, and is cultivated at 26°C for 7 days, and the total number of bacterial communities contained in each milliliter of anthrax spore suspension 1×10 8 CFU / mL;

[0033] St...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a disease resistance intensity identifying method for resistance to tobacco anthracnose. The method comprises the following steps: step (I), planting a to-be-identified tobacco variety, a known anthracnose-resistant variety, a moderate anthracnose-resistant variety and a known anthracnose sensing variety in a tobacco field; step (II), transplanting indoors; when true leaves grow on the tobacco seedling, inoculating anthracnose spores; and after the inoculation, growing under the environmental conditions that the temperature is 26-30 DEG C and the relative humidity is over 80% to induce attack; and step (III), detecting the disease resistance intensity of resistance to each variety anthracnose. The invention provides a disease resistance intensity identifying method for resistance to tobacco anthracnose, which can effectively identify the disease resistance intensity of anthracnose to a tobacco variety.

Description

technical field [0001] The invention relates to a method for identifying the strength of tobacco resistance to anthracnose. Background technique [0002] Tobacco anthracnose is caused by fungal infection of the genus Ignomonia subhexapia. After tobacco anthrax was first reported by Brazil in 1922, it was also discovered in Germany, Japan, the United States, China, Australia, India, North Korea and Africa. The disease can occur in all growth stages of tobacco, but it is common and serious at the seedling stage. The leaves of seedlings are densely covered with diseased spots, and the whole tobacco seedlings are often destroyed when the disease is severe. Generally, although the seedlings are not destroyed when the disease occurs, the seedlings have poor growth potential, and they can continue to cause damage after transplanting to the field, resulting in greater losses. The occurrence of anthracnose has seriously affected the economic benefits of tobacco cultivation. How to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G1/00A01G7/06
CPCA01G7/06
Inventor 陈泽鹏
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap