Disease resistance intensity identifying method for resistance to tobacco anthracnose
An anthracnose resistance and identification method technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of destroying tobacco seedlings, poor seedling vigor, affecting the economic benefits of tobacco planting and the like
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Embodiment 1
[0020] The method for identifying the strength and weakness of tobacco resistance to anthracnose comprises the following steps:
[0021] Step 1, planting tobacco varieties to be identified, known anthracnose-resistant varieties, moderate anthracnose-resistant varieties and known anthracnose-susceptible varieties in the tobacco field;
[0022] Step 2: Transplant indoors, inoculate the anthracnose spores when the tobacco seedlings grow 5 true leaves, spray the spore suspension onto the plants with a small hand-held sprayer, so that the front and back of the leaves are fully moist, and keep the temperature at 26°C after inoculation , growing under environmental conditions with a relative humidity of more than 80%, and inducing morbidity; wherein, the anthrax spore suspension adopts potato dextrose agar (PDA) medium, and is cultivated at 26°C for 6 days, and the total number of bacterial communities contained in each milliliter of anthrax spore suspension 0.8×10 8 CFU / mL;
[002...
Embodiment 2
[0025] The method for identifying the strength and weakness of tobacco resistance to anthracnose comprises the following steps:
[0026] Step 1: Plant tobacco varieties to be identified, known anthracnose-resistant varieties, moderately anthracnose-resistant varieties, known anthracnose-susceptible varieties, local anthracnose-resistant varieties, local anthracnose-resistant varieties and local anthracnose-susceptible varieties in the tobacco field ;
[0027] Step 2: Transplant indoors, inoculate the anthracnose spores when the tobacco seedlings grow 6 true leaves, spray the spore suspension onto the plants with a small hand-held sprayer to make the front and back of the leaves fully moist, and keep the temperature at 30°C after inoculation , grow under environmental conditions with a relative humidity of 80%, and induce disease; wherein, the anthrax spore suspension adopts potato dextrose agar (PDA) medium, and is cultivated at 26°C for 6-8d, and the bacteria contained in eac...
Embodiment 3
[0030] The method for identifying the strength and weakness of tobacco resistance to anthracnose comprises the following steps:
[0031] Step 1, planting tobacco varieties to be identified, known anthracnose-resistant varieties, moderate anthracnose-resistant varieties and known anthracnose-susceptible varieties in the tobacco field;
[0032] Step 2: Transplant indoors, inoculate the anthracnose spores when the tobacco seedlings grow 5 true leaves, spray the spore suspension onto the plants with a small hand-held sprayer to make the front and back of the leaves fully moist, and keep the temperature at 28°C after inoculation , growing under environmental conditions with a relative humidity of more than 80%, and inducing morbidity; wherein, the anthracis spore suspension adopts potato dextrose agar (PDA) medium, and is cultivated at 26°C for 7 days, and the total number of bacterial communities contained in each milliliter of anthrax spore suspension 1×10 8 CFU / mL;
[0033] St...
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