Gene set for liver cancer detection and detection and design method of panel of gene set

Abstract

The invention discloses a preparation method of a liver cancer detection panel. The preparation method comprises the following steps: screening liver cancer information and related genes thereof, thusobtaining a gene set; selecting a gene target zone; designing a panel probe; synthesizing an RNA (Ribonucleic Acid) single-stranded probe, thus obtaining the gene detection panel. The gene detectionpanel designed by the invention can be used for diagnosis, typing and prognosis of liver cancer, has wide gene coverage degree and strong transferability and is suitable for newly-developed detectionplatforms.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a gene set for liver cancer detection and a panel detection design method thereof, and also relates to the application of the gene set in a gene chip. Background technique [0002] Liver cancer is one of the most common malignant tumors in clinical practice. According to statistics, there are about 740,000 new cases of liver cancer and 700,000 deaths from liver cancer every year worldwide, about half of which occur in China. Due to the high morbidity and mortality of liver cancer, liver cancer is paid special attention worldwide, and the incidence and mortality of liver cancer in my country are also increasing year by year. Liver cancer ranks second in the overall cause of death related to various malignant tumors. Seriously endangering human health, it has become a global and serious public health problem. The occurrence and development of liver cancer is a complex biol...

AI Technical Summary

Problems solved by technology

The technology of diagnosing liver cancer by high-throughput sequencing of the genome in biological samples has made great progress, but the existing liver cancer detection has the following defects: 1. The detection content is single and the function is limited

At present, most panel designs are only aimed at drug guidelines, labels, and limited literature collection, and there are few comprehensive analyzes based on the results of large-scale sequencing platforms, public databases, and text mining techniques

3. At present, the technical process of panel design and synthesis method is single, and there are always areas that cannot be designed according to the fixed algorithm, so it is inevitable to omit some important sites

4. There are few applicable platforms. For the developed panel detection, there is already a set of detection system schemes on common platforms, but for new detection platforms, such as BGISEQ-500, there is no suitable detection scheme yet

5. The process of information analysis method is cumbersome

The bioinformatics analysis of off-machine data needs to be evaluated step by step, and the results are completed step by step. The steps are cumbersome, time-consuming, and the portability is not high, which cannot meet the current analysis needs.

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