Plant development associated protein, encoding gene and application thereof

A technology for encoding genes and plant development, which is applied in the field of plant development-related proteins and their encoding genes and applications, can solve problems such as increasing yield, and achieve the effects of increasing plant yield and improving plant type

Inactive Publication Date: 2010-07-21
INST OF BOTANY CHINESE ACAD OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After screening more than 100 mutants, people found the weak mutant d61-7 of OsBRI1. Although the mutant can increase the biomass of rice by dense planting, it does not increase the yield because d61-7 produces small seeds.

Method used

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  • Plant development associated protein, encoding gene and application thereof
  • Plant development associated protein, encoding gene and application thereof
  • Plant development associated protein, encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1, the discovery of OsILI1

[0063] 1. Obtaining and Morphological Observation of Mutant Plant ili1-D

[0064] 1. Obtaining of mutant plants ili1-D

[0065] A Nipponbare rice T-DNA insertion mutant library was constructed, and a mutant plant ili1-D with a significantly increased leaf angle was found.

[0066] 2. Morphological observation of mutant plants ili1-D

[0067] The following morphological observations were performed on the mutant plant ili1-D.

[0068] (1) leaf angle

[0069] The most important phenotype of ili1-D was that the leaf angle increased significantly at each growth stage. At the seedling stage, the mutant grew for about 5 days after germination, and after the second leaf was fully developed, it could be observed that the angle between the second leaf and the leaf angle was bent more than 90 degrees (see figure 1 ). At the tillering stage, each new tiller of the mutant showed a significantly increased leaf angle (see figure 2 ).

[00...

Embodiment 2

[0094] Embodiment 2, the discovery of OsIBP1

[0095] 1. Discovery of OsIBP1

[0096] According to the analysis of OsILI1 protein sequence, OsILI1 belongs to the bHLH family of transcription factors, but it does not have a typical basic domain. Such bHLH proteins are classified as class D bHLH proteins, which cannot directly bind DNA and regulate their functions mainly by forming heterodimers with canonical bHLH transcription factors. Using the full-length OsILI1 protein as bait, yeast two-hybrid technology was used to screen for proteins interacting with OsILI1. After the coding sequence of OsILI1 was amplified from the cDNA library, it was connected into the pBD-GAL4 vector, and then the constructed vector was transformed into yeast AH109, ​​and after being made into competent cells, the yeast two-hybrid library at the three-leaf stage of rice was co-transformed. The efficiency of the sieve library is 3×10 5 Clones / g DNA, the total amount of screening library is 3×10 6 C...

Embodiment 3

[0107] Embodiment 3, the acquisition of OsIBP1 overexpression rice

[0108] 1. Construction of recombinant expression vector

[0109] 1. Obtaining the 35S promoter fragment

[0110] Plasmid vector pBI221 (Clontech) was double digested with restriction endonucleases HindIII and BamHI, and a 35S promoter fragment with a length of about 0.8 kb was recovered after detection by agarose gel electrophoresis.

[0111] 2. The Noster poly A termination sequence was excised from the plasmid vector pBI221 (Clontech) with restriction enzymes Sac I and EcoR I, and connected to the corresponding site of the vector pUC19 (TaKaRa Company) to obtain a recombinant vector named as pUC19-Noster. Restriction endonucleases HindIII and BamHI were used to double digest pUC19-Noster, and after detection by agarose gel electrophoresis, the large linearized vector fragment was recovered, and the recovered fragment was connected with the 35S promoter fragment obtained in step 1 to obtain recombinant ve...

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Abstract

The invention discloses plant development associated protein, an encoding gene and application thereof. The protein provided by the invention is the protein of (a) or (b): (a) the protein formed by amino acid sequences shown as a third sequence in a sequence table; or (b) plant development associated derived protein formed through the substitution and / or lack and / or addition of one or a plurality of amino acid residue radicals on the protein of the third sequence in the sequence table. The plant development is embodied by the properties of the strain height of developed strains and / or the size of leaf angles and / or the fertility of the developed strains and / or the size of specific organs. The invention also obtains a reccanbinant vector containing the encoding gene OsIBP1, and when the reccanbinant vector is used for converting target plants, transgenic plants with shortened strain height of the developed strains and / or reduced leaf angles and / or reduced fertility of the developed strains and / or reduced size of the specific organs can be obtained. Thereby, OsIBP1 can be used as a potential molecular breeding tool for improving the plant strain types and improving the plant yield.

Description

technical field [0001] The invention relates to a plant development-related protein, its coding gene and application. Background technique [0002] Ideal plant architecture is the decisive factor for increasing rice yield. In the ideal plant type of rice, semi-dwarf and erect leaves are two very favorable traits for increasing yield (Wang Y and Li J,. 2008, Annu. Rev. Plant Biol., 59: 253-279). The Green Revolution is a successful example of improving rice yield by improving rice plant type. Some recent studies have shown that leaf erection is also one of the goals of achieving high-yielding rice plant architecture. Upright leaves can increase the photosynthetic rate of the canopy, improve the illumination of the lower part of the group, and increase the material production; at the same time, it can increase the amount of light at the base of the canopy, enhance the vitality of the root system, and improve the lodging resistance; it is also conducive to dense planting and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/00C12N15/82
Inventor 王志勇种康路铁刚白明义张丽颖朱佳瑛
Owner INST OF BOTANY CHINESE ACAD OF SCI
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