Plant-related gene from paddy and its coded protein and application thereof

A gene and plant type technology, applied in the application field of improving plant type, can solve problems such as unknown function

Inactive Publication Date: 2010-07-14
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to our research progress, the isolated Nal1 candidate gene is a new type of gene with unknown function, which may encode a new type of receptor protein. In-depth research on its function will provide innovation for understanding the morphogenesis mechanism of rice leaves. Accumulation of knowledge about nature and the potential to open up new research directions

Method used

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  • Plant-related gene from paddy and its coded protein and application thereof
  • Plant-related gene from paddy and its coded protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Acquisition of the plant-type-related gene Nal1 derived from rice

[0046] 1. Obtaining rice nal1 mutants

[0047] A near-isogenic line of narrow-leaf dwarf material was obtained through about ten generations of backcrossing the early indica rice variety Zhefu 802 (Dong Fenggao, Xiong Zhenmin, Qian Qian, Zu Xudong, Chen Shihua (1994). Breeding Near- isogenic lines of morphological markers in indica rice. ChineseJ.Rice Sci.8, 135-139. The mutant can be purchased from the China Rice Research Institute), and the mutant material is named nal1 (narrow leaf 1), which is similar to the normal plant The most prominent phenotypes of nal1 were that the height of the main panicle (52±3.5cm) was significantly shorter than that of the normal plant (74±3.5cm), the leaves were narrower and darker, and the number of spikes per plant (22.5±1.8) was shorter than that of the normal plant (11.7±2.4) significantly increased.

[0048] 2. The plant type-related gene Nal1 from the...

Embodiment 2

[0056] Example 2. Confocal analysis of normal wild-type plants and nal1 mutants

[0057] The leaves of the normal wild-type Zhefu 802 plants (control, WT) and the nal1 mutant and the significantly shortened fourth internode were selected for con-electrofocus analysis, and the results were as follows: Figure 4 As shown (bar=100um), the total number of small vascular bundles (sv) in nal1 mutant leaves (blade) was significantly reduced compared with wild-type normal plants, and at the same time, two adjacent large vascular bundles (lv) in nal1 mutant leaves (blade) ), the number of small vascular bundles (sv) between nal1 mutants was also significantly reduced compared with that of wild-type normal plants, resulting in significantly narrower leaves of the nal1 mutant. The above detection results indicated that Nal1 gene may affect the width and size of leaves by regulating the formation of small vascular bundles (sv) in leaves (blade), and Nal1 gene also plays an important role ...

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Abstract

The invention discloses a relative gene and coded protein and application of rice and strain type, wherein the cDNA sequence of gene comprises the following sequences: 1) DNA sequence in the SEQ ID No:1; 2) DNA sequence in the SEQ ID No:2; 3) DNA sequence with 90% isogenesis corresponding to SEQ ID No:1 with modified plant strain nucleotide sequence; 4) crossing nucleotide sequence to limit DNA sequence in the SEQ ID No:1 under high strict condition.

Description

technical field [0001] The present invention relates to a gene related to plant type and its encoded protein and its application, in particular to a gene related to plant type derived from rice, its encoded protein and its application in improving plant type. Background technique [0002] Plant type refers to a group of characteristics related to the yield capacity of crop varieties or the arrangement of plants in space, that is, the growth and appearance. Ideal plant type (Ideal plant type), also known as ideal type (Ideotype), refers to the ideal plant type composed of traits that are beneficial to plant photosynthesis, growth and development, and grain yield, which can maximize the use of light energy in the group , increase biological yield and improve economic coefficient, etc. Regarding the research on rice plant type, in the late 1950s, Japanese scientist Shigesaburo Kakuda concluded from his research practice on rice, soybeans, sugarcane, etc. that the varieties sui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N5/10C12N15/82
Inventor 李传友齐静钱前孙加强李常保蒋红玲吴晓燕
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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