Method for constructing pepper mutant library from ethylmethane sulfonate

Abstract

The invention discloses a method for constructing a pepper mutant library from ethylmethane sulfonate. The method is characterized in that a Zunla 1 is used as a mutagenesis object; on the premise ofpointing out the influence of treating fluid dose and excluding space of each seed on germination percentage, semi-lethal dose is determined by comparing the germination percentage of pepper seeds with EMS treating fluid with different concentrations at different mutagenesis time; the pepper seeds are treated by mutagenesis of the semi-lethal dose; mutation frequency and mutation types of M2 generation are investigated, a mutant capable of stably inheriting of M4 generation is identified and a mutant library is constructed; the mutation types of leaves, stems, fruits, growth period, flower organs, fertility and the like are obtained and create abundant materials for functional genomics reach of pepper; and meanwhile, partial beneficial mutation can be directly applied to breeding practice.

Description

technical field [0001] The invention belongs to the technical field of crop mutation breeding, and relates to a method for constructing a capsicum mutant library by using ethyl methanesulfonate. Background technique [0002] Pepper is one of the most important vegetable crops in my country, and its planting area accounts for about 10% of the total vegetable planting area, which plays an important role in ensuring the annual balanced supply of vegetables in our country. Guizhou has the largest planting area of ​​pepper in the country, and the planting area of ​​Chaotian pepper accounts for about 40% of the total pepper planting area in the province. Therefore, Chaotian pepper occupies a pivotal position in the pepper industry in Guizhou Province and even the whole country. What's more worth mentioning is that Guizhou chaotian pepper has a strong aroma and moderate spiciness, and its unique flavor quality is irreplaceable for Guizhou people. Although Chaotian pepper has outst...

AI Technical Summary

Problems solved by technology

Although Chaotian pepper has outstanding flavor quality and industrial advantages, there are still some deficiencies in production practice: first, the wrinkled fruit surface reduces the commercial quality of the fruit; Third, the loose plant type is not conducive to reasonable dense planting, which affects the high yield of pepper

However, ethyl methanesulfonate has little research on capsicum breeding, and there is no relevant report on the mutant library of Chaotian pepper

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