Paddy rice autotetraploid hybrid viger utilization and its propagation seed making technology

A technology of autotetraploidy and heterosis, applied in the fields of application, botany equipment and methods, and plant gene improvement, can solve problems such as retention, achieve increased yield, huge economic and social benefits, and far-reaching theoretical and practical significance Effect

Inactive Publication Date: 2003-09-17
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Looking at the utilization of heterosis, although there have been continuous improvements and innovations in methods and technologies, they still remain at the diploid level and have not been able to break through the limitation of the ploidy level.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: The inventor began to use rice as a material to explore this technology in 1996, using various excellent diploid rice materials such as Minghui 63 and RT2248, and carried out 0.1% colchicine at the cellular level Doubled, more than 40 kinds of autotetraploid rice and more than 300 selected lines have been successfully created.

[0034] From 1996 to 2001, the autotetraploid rice materials created by biotechnology were carried out in Lingshui County, Hainan Province, and Shuangliu Farm, Chengdu Institute of Biology, Chinese Academy of Sciences, for multi-generational conventional hybridization and backcross improvement. Seed setting rate, plumpness and agronomic traits have been significantly improved, among which the highest seed setting rate is 90%, and one-third of the seed setting rate is above 70%. Some autotetraploid materials can basically meet the actual production requirements. needs.

[0035] At the same time, 4 sets of autotetraploid rice male steri...

Embodiment 2

[0036]Example 2: From 1998 to 2002, in Shuangliu Farm, Chengdu Institute of Biology, Chinese Academy of Sciences, and Wenjiang High-tech Biotechnology Experimental Base, Chengdu Institute of Biology, Chinese Academy of Sciences, the combination of autotetraploid rice male sterile three lines was carried out. Matching and screening, nearly 200 combinations were trial-produced, and the world's first batch of super-dominant autotetraploid hybrid rice combinations such as D46A×D237 and D46A×D71 were bred, namely super rice. Among them, the output of the former plot is equivalent to 1015 kg per mu, which is 33% higher than that of the control D You 527 mu, which is 761 kg.

[0037] The breeding and seed production experiments carried out by the inventor are similar to the breeding and seed production principles of the common diploid rice male sterile three lines. At present, field experiments are being stepped up to improve its reproduction and yield.

[0038] During reproduction ...

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PUM

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Abstract

An application of the homogenetic tetrapolid hybrid vigor of paddy rice in preparing seeds includes such steps ad artificial reduplication of excellent diploid rice to obtain homogenetic tetraploid, improving by several generations, selectively culturing the trilinear homogenetic tetraploid male sterility line, further improving, culturing the homogenetic tetraploid hybrid combination and the newvariety with ultra-strong dominance, sterile reproduction between homogenetic tetraploid metal sterility line and maintenance line, and preparing the homogenetic tetraploid hybrid rice seeds with homogenetic tetraplid male sterility line and recovery line. The resultant seed features, ultra-strong hybrid vigor, high resistance and adaptability, and high protential to increase yield.

Description

[0001] 1. Fields: [0002] The invention belongs to the new technique of polyploid heterosis utilization. 2. Background technology [0003] Plant polyploid phenomenon widely exists in nature, and the proportion of polyploid in gramineous plants is as high as 3 / 4. Polyploid has the advantages of luxuriance, large size, high resistance, strong adaptability, and high effective substance content. It has always been the goal pursued by breeders for a long time. The discovery of the doubling effect of colchicine in 1937 opened the door for the artificial induction and utilization of polyploidy. Over the past 60 years, great achievements have been made. Tetraploid rye, tetraploid sugar beet, tetraploid rubber grass, tetraploid grape, triploid watermelon, hexaploid and octoploid triticale have been applied Production. Chinese scholar Bao Wenkui carried out research on rice autotetraploid as early as the early 1950s, and pointed out that rice autotetraploid hybrids may exhibit heter...

Claims

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Application Information

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IPC IPC(8): A01H1/04
Inventor 孔繁伦涂升斌徐琼芳高朝龙
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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