Rice leaf rolling-associated protein OsMYB103L as well as encoding gene and application thereof

A gene and curling technology is applied to the rice leaf curling-related protein OsMYB103L and its encoding gene and application fields, so as to achieve the effect of improving plant type and increasing rice yield

Inactive Publication Date: 2013-06-26
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many genes that may control rice leaf curl have been reported, but most of them have only been initially mapped to a certain segment of the rice chromosome

Method used

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  • Rice leaf rolling-associated protein OsMYB103L as well as encoding gene and application thereof
  • Rice leaf rolling-associated protein OsMYB103L as well as encoding gene and application thereof
  • Rice leaf rolling-associated protein OsMYB103L as well as encoding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Discovery of rice leaf curl-related protein OsMYB103L and its encoding gene

[0041] Systematic reverse genetics research on several rice MYB genes pre-screened from the japonica rice variety Nipponbare (Oryza sativa L.subsp.japonica cv.Nipponbare), the main research strategy is to overexpress and / or RNAi the target gene analyze. A novel protein and its coding gene were discovered during phenotypic analysis of transgenic rice populations.

[0042] The protein shown in Sequence 1 of the Sequence Listing was named OsMYB103L protein (consisting of 359 amino acid residues). The domain analysis of OsMYB103L protein on the protein conservation domain analysis website showed that there were two tandem MYB DNA binding domains (R2 and R3) at its N-terminus. Systematic evolution of the OsMYB103L protein and the related R2R3MYB protein of Arabidopsis thalina (Arabidopsis thalina L.) revealed that it has the highest homology with AtMYB103 (At1g63910), and the two are hig...

Embodiment 2

[0044] Example 2. Functional verification of OsMYB103L protein as a transcription factor

[0045] 1. Localization of OsMYB103L protein in plant cells

[0046] Transcription factors generally perform their biological functions in the nucleus. In order to analyze the localization of the OsMYB103L protein in cells, the 3' end of the full-length ORF of the OsMYB103L gene (the DNA shown in Sequence 2 of the Sequence Listing without the stop codon) was combined with the binary vector pEZR(K)-LN (Brown et al , PNAS, 2005, 102:18225-18230) was fused with the green fluorescent protein gene eGFP and placed under the control of the CaMV 35S promoter, thereby constructing a 35S::OsMYB103L-eGFP expression vector. The recombinant vector and the control empty vector were respectively introduced into the inner epidermis of the onion by a gene gun-mediated method, and after 24 hours of culture, the eGFP fluorescence signal was observed under a laser confocal microscope. The green fluorescent...

Embodiment 3

[0060] Embodiment 3, the expression analysis of OsMYB103L gene

[0061] To study the expression pattern of the OsMYB103L gene, it was first analyzed by quantitative RT-PCR in the roots, stems, leaves, young panicles (less than 5 mm in length) and mature panicles of the indica rice variety Kasalath (Oryza sativa L. subsp. indica cv. Kasalath). expression in other tissues. The fluorescent signal (expressed in Ct value) of OsMYB103L gene and the fluorescent signal (expressed in Ct value) of Actin1 gene (internal reference gene) were calculated according to the formula (relative expression amount=2 -ΔCt , where ΔCt=Ct 目的基因 -Ct 内参基因 ) calculated value as the relative expression level of OsMYB103L gene, the results are shown in image 3 . The results showed that the OsMYB103L gene was expressed in roots, stems, leaves and mature ears, with the highest expression level in stems, relatively low expression levels in roots, leaves and mature ears, and basically no expression in youn...

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Abstract

The invention discloses a rice leaf rolling-associated protein OsMYB103L as well as an encoding gene and application thereof. The protein provided by the invention is (a) or (b): (a) the protein is composed of the amino acid sequence shown in the sequence 1 in a sequence table; (b) the protein is associated with the plant leaf rolling character and derived from the sequence 1 through carrying out the substitution and / or deletion and / or addition of one or several amino acid residues on the amino acid sequence of the sequence 1. When the rice leaf rolling-associated gene is over-expressed, the rice leaves roll inwards, but the characters such as fertility and the like of the transgenic rice are not significantly influenced and no other undesirable agronomic characters are observed, so that a significant potential function is provided to further improve the rice yield. The gene disclosed by the invention and the encoded protein thereof play significant roles in cultivating the plant varieties with properly rolled leaves and improving the plant types.

Description

technical field [0001] The invention relates to a rice leaf curl-related protein OsMYB103L and its coding gene and application. Background technique [0002] Rice is one of the most important food crops in the world, and improving yield has always been the main purpose of rice breeding. Plant type is an important factor affecting rice high yield, and leaves are an important part of plant structure. In the process of rice high-yield breeding and plant type improvement, leaf traits have always been the focus of breeders. The improvement of leaf shape It has always been one of the important goals of plant type breeding. [0003] At present, it is considered that the moderate curling of leaves is an important factor for the ideal plant type of rice, and this trait has been directly or indirectly applied to the improvement of plant type of rice and the breeding of hybrid rice. The moderate curling of the leaves is conducive to keeping the leaves straight, while reducing the lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/21C12N1/19C12N15/11C12N15/82C12N15/84A01H5/00
Inventor 朱立煌杨春花李大勇刘雪
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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