Method for directionally and selectively separating intestinal bacteria

A technology for directional separation of intestinal bacteria, applied in the direction of separation of microorganisms, biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as long-term, labor-consuming, and inability to answer microbial community functions and metabolism problems

Active Publication Date: 2019-08-06
XINXIANG MEDICAL UNIV
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Problems solved by technology

[0004] At present, since simple gene sequencing cannot answer questions about the function and metabolism of microbial communities, it is even more difficult to clarify the functions of microorganisms closely related to humans, especially intestinal flora, in the human body. Therefore, the isolation of uncultured microorganisms method is gaining more and more attention from researchers
Although the development

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  • Method for directionally and selectively separating intestinal bacteria
  • Method for directionally and selectively separating intestinal bacteria
  • Method for directionally and selectively separating intestinal bacteria

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Embodiment 1

[0042] The method for directional and selective isolation of intestinal bacteria (Bacteroides) in the present embodiment comprises the following steps:

[0043] 1. Cultivate the samples to be separated on different culture media to obtain specific culture flora.

[0044] 1) Take some feces (about 0.1g) of three animals with the same background (number: 37, 39, 52) and put them into 2mL centrifuge tubes respectively, add 1mL of normal saline for each 0.1g of feces, stir and shake in a vortex instrument 5min, diluted with normal saline to 10% of feces -6 and 10 -7 times.

[0045] 2) Prepare 8 kinds of common intestinal flora medium solid plate medium, improved GAM medium (GAM), brain heart medium (BH), cooked meat medium (CM), nutrient broth medium (NA ), soybean casein agar medium (TSA), enhanced Clostridium medium (RCM), Mueller-Hinton medium (MH) were purchased from Qingdao Haibo Biological Company, and blood culture medium (BL) was purchased from Guangdong Huankai Biologi...

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Abstract

The invention relates to a method for directionally and selectively separating intestinal bacteria, and belongs to the technical field of microbial separation. The invention provides a method for directionally and selectively separating intestinal bacteria. The method comprises the following steps: before separating samples, performing high-throughput sequencing on samples and samples growing in different culture media; selecting samples with high abundance content of required strains through bioinformatics standardized analysis; and mastering the requirements of nutritional ingredients and the interaction relationship among the bacteria so as to increase or reduce corresponding nutrient substances and bacterial fermentation liquor, and performing directional separation to obtain the required strains.

Description

technical field [0001] The invention relates to a method for directional and selective separation of intestinal bacteria, which belongs to the technical field of microorganism separation. Background technique [0002] Gut flora is a hotspot in the field of human health research. Intestinal bacteria are the microorganisms with the largest number in the human body and the most closely related to human health. According to existing research data, there are more than 1,000 species of bacteria in the human intestinal tract, with a total weight of about 1.5kg and a total of about 10 bacteria. 14 1, which is 10-20 times of the total number of tissue cells in the human body. There are as many as 3 million microbial genes in the human intestinal tract, which is 150 times that of its own genes (Byrd, A.L., Belkaid, Y. & Segre, J.A. The human skinmicrobiome. Nat. Rev . Microbiol. 16, 143-155 (2018)). Intestinal flora is an important part of the intestinal micro-ecosystem. Usually, a...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12Q1/10C12Q1/689
CPCC12N1/02C12Q1/689C12Q1/10Y02A50/30
Inventor 闫冬谷妍蓉吴敏娜李敏杨帆王晶晶
Owner XINXIANG MEDICAL UNIV
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