Method for directionally and selectively separating intestinal bacteria

Abstract

The invention relates to a method for directionally and selectively separating intestinal bacteria, and belongs to the technical field of microbial separation. The invention provides a method for directionally and selectively separating intestinal bacteria. The method comprises the following steps: before separating samples, performing high-throughput sequencing on samples and samples growing in different culture media; selecting samples with high abundance content of required strains through bioinformatics standardized analysis; and mastering the requirements of nutritional ingredients and the interaction relationship among the bacteria so as to increase or reduce corresponding nutrient substances and bacterial fermentation liquor, and performing directional separation to obtain the required strains.

Description

technical field [0001] The invention relates to a method for directional and selective separation of intestinal bacteria, which belongs to the technical field of microorganism separation. Background technique [0002] Gut flora is a hotspot in the field of human health research. Intestinal bacteria are the microorganisms with the largest number in the human body and the most closely related to human health. According to existing research data, there are more than 1,000 species of bacteria in the human intestinal tract, with a total weight of about 1.5kg and a total of about 10 bacteria. 14 1, which is 10-20 times of the total number of tissue cells in the human body. There are as many as 3 million microbial genes in the human intestinal tract, which is 150 times that of its own genes (Byrd, A.L., Belkaid, Y. & Segre, J.A. The human skinmicrobiome. Nat. Rev . Microbiol. 16, 143-155 (2018)). Intestinal flora is an important part of the intestinal micro-ecosystem. Usually, a...

AI Technical Summary

Problems solved by technology

[0004] At present, since simple gene sequencing cannot answer questions about the function and metabolism of microbial communities, it is even more difficult to clarify the functions of microorganisms closely related to humans, especially intestinal flora, in the human body. Therefore, the isolation of uncultured microorganisms method is gaining more and more attention from researchers

Although the development and application of in situ culture technology, co-culture technology and culture-omics technology have isolated and obtained microorganisms that were previously considered unculturable, these methods often take a long time, consume a lot of manpower, and blindly isolate from a large number of samples. Unknown microorganisms, and 90% of the strains that may be obtained are repeated strains that have been isolated

Images