Breeding method of new strain of sweet-waxy recessive gene corn selfing line
The technology of a recessive gene and an inbred line is applied in the breeding field of a new corn inbred line with a sweet-waxy recessive gene, and can solve the problems of narrow germplasm genetic basis, poor flavor and taste, poor disease resistance and stable yield, etc. Achieve the effect of being beneficial to effective utilization, good bract wrapping, and reducing grain rot.
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Embodiment 1
[0025] Example 1 : The breeding method of corn line NB group
[0026] (1) SN512, JN20-32, NB5B111, JNF20-K, Zhengnuo 61, Zhengnuo 621, Jingnuo F20, Jingnuo 12, Liunuo 587-111, Zhenzhunuo 22, Zhennuo 411, Zhenzhunuo in the spring of 2003 Nuo 31, DW586, Miscellaneous Sweet Flower Nuo White, Miscellaneous Sweet Flower Nuo Purple, Xiang 421, Cai Tian Nuo 12, Cai Tian Nuo 31, Jing Ke Nuo 42, Zheng Bai Nuo 35, Lai Nuo 15, Xi Xing Nuo 23, Black Nuo 4-2, Hei Nuo 5-2, Lu Yunuo-21, Xixing 131 mixed, Jingke Nuo 2-2, Zi Nuo 1, Zheng Hei Nuo-2, Hei Nuo-11, Jing Nuo Tian 21, Crystal Nuo-31, Tian Nuo 1, Zheng Nuo 11, Lijing 11, Zhenzhu Nuo 31, Mei Nuo Zajing, Mei Nuo 12, y5-1 Nuo, Zhen Nuo 512 and more than 40 self-bred lines were analyzed by genetic diversity ;
[0027] (2) In the autumn of 2003, according to the results of genetic diversity analysis, 9 species including SN512, JN20-32, NB5B111, JNF20-K, Zhengnuo 61, Zhengnuo 621, Jingnuo F20, Jingnuo 12 and Liunuo 587-111 were selected....
Embodiment 2
[0029] Example 2 : Breeding method of super sweet waxy double recessive waxy corn inbred line WT1791
[0030] (1) In the autumn of 2005, the inventors selected the waxy corn line NB group to cross with super sweet waxy corn combination Huatiannuo 179 containing super sweet gene (sh2 / sh2) as the basic material for line selection. From the spring of 2006 to the spring of 2007, the pedigree method combined with molecular marker-assisted directional selection was used to select the superior plants containing the super sweet and waxy double recessive gene for selfing and selection to the S3 generation. In this step, the planting density was selected to be 60,000 plants / hm 2 ;
[0031] (2) In the autumn of 2007, the combining ability of the S3 strains was determined, and in the autumn of 2008, strains such as NB05-3-6-1 and NB05-5-1-2 were identified. In this step, the planting density was selected as 60000 plants / hm 2 ;
[0032] (3) In the spring of 2009, the circular breeding...
Embodiment 3
[0034] Example 3 : Stress resistance, quality and utilization of WT1791
[0035] (1) Stress resistance of WT1791
[0036] The Institute of Plant Protection of Guangxi Academy of Agricultural Sciences identified the stress resistance of WT1791. The identification method was carried out according to conventional techniques. The identification results are as follows: the inoculation level of southern rust is 3, which belongs to resistance; , belong to high resistance; small spot disease inoculation identification is grade 3, which belongs to resistance; . Seedling pot plant drought-rewatering test in 2014 and 2015 and observation results of field drought tolerance for many years, the drought tolerance is above average.
[0037] (2) Quality of WT1791
[0038] WT1791 has white, darker grains, double recessive genotype of super sweet and waxy grains, and good appearance.
[0039] (3) Utilization of WT1791
[0040] WT1791 cannot be directly applied in production, but can be ap...
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