Method for producing mutant of hemerocallis middendorffii by in vitro mutagenesis of ethyl methane sulfonate

Abstract

A method for producing mutant of hemerocallis middendorffii by in vitro mutagenesis of ethyl methane sulfonate (EMS) amis to provide an EMS in vitro mutagenesis method for breed improvement of hemerocallis and creation of new germplasm resources and to obtain a target variant strain. The method for producing the mutant of the hemerocallis middendorffii by the in vitro mutagenesis of the ethyl methane sulfonate adopts the 1 to 2cm flower buds in normal growth and development state of the hemerocallis middendorffii as materials, inoculates callus induced by taking ovaries as explants to a differential medium, and green buds are differentiated. After the callus with buds is subjected to differentiation culture for 10 days, a callus block is treated with EMS semi-lethal dose at a concentration of 0.75-1.0 percent (w/v). The treated callus is inoculated to the differentiation medium for differentiation culture to obtain regenerated plants. When the regenerated plants grow to 1 to 2 cm high, the regenerated plants are cut down from the callus and put in a subculture medium to conduct subculture for 15 days, hemerocallis septoria tritici toxin semi-lethal dose at a concentration of 40 percent (v/v) is taken as directional selection pressure for stress screening, and disease-resistant mutant plants are obtained.

Description

technical field [0001] The invention relates to a method for in vitro inducing mutants of a flower tree, Hemerocallis grandiflorum, specifically a method for mutagenizing callus of Hemerocallis grandiflorum by using a chemical mutagen ethyl methanesulfonate (EMS), Technical methods for inducing mutants. Background technique [0002] Hemerocallis has a history of thousands of years of cultivation in our country. Hemerocallis is also known as scorpion grass, which means to forget, and it is also called forgetting grass. Hemerocallis hybrida is a perennial herb of the genus Hemerocallis in the family Liliaceae. Its leaves are like orchids, and its flowers are like lilies. There are more than ten kinds of flower colors, such as light yellow, golden yellow, light yellow, bright red, pink, double color, etc., which are gorgeous and colorful. Hemerocallis grandiflora has excellent cultivation characteristics in the dry and cold regions of the north: it has short rhizomes and fle...

AI Technical Summary

Problems solved by technology

The research on the generation of mutants by EMS in vitro mutagenesis of receptor materials has been widely carried out in crops and horticultural plants, but there

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