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Breeding method for hybrid rice three-line male sterile line with recessive marker and application thereof

A technology of three-line male sterile line and hybrid rice, which is applied in the field of breeding of three-line male sterile line of hybrid rice with recessive markers, and achieves the effect of fast parental selection, improved parental purity, and improved breeding efficiency

Inactive Publication Date: 2012-01-18
江西现代种业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no example of using the recessive marker-complex marker. The present invention provides a new, intuitive and accurate method for the identification of hybrid rice seeds

Method used

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  • Breeding method for hybrid rice three-line male sterile line with recessive marker and application thereof
  • Breeding method for hybrid rice three-line male sterile line with recessive marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In 2005, a farm variety with compound glume markers - Maiyingdao was collected. In the winter of 2005, Maiyingdao was crossed with the three-line male sterile line Tianfeng A in Hainan, and it was planted in Ningdu in 2006. F 1 On behalf of non-compound sterility.

[0024] F 1 Generation, planted F in Hainan in the winter of 2006 1 , F 1 Representing Fafei Fuying, planted F in Ningdu in May 2007 2 , there are two types of compound glumes and non-complex glumes when heading, and the seeds of the compound glumes are selected, and they are sown in Hainan in October (F 3 ), after 6 generations of selfing, wheat B was selected as the maintainer line from the progeny with compound glumes, high rice quality, and good plant and leaf shape.

[0025] Planted F in April 2008 6 F 1 , sowing F in Ningdu in May 2008 1 , all were sterile when heading in August, backcross (Maiyingdao × Zhongzhe B) F 7 There are 6 single plants in the surrogate parent, and the plants with compou...

Embodiment 2

[0027] The wheat A (compound glume) bred by the present invention is crossed with the strong restorer line Huazhan (non-compound glume) bred by Chinese rice, and Mai A / Huazhan is the combination (variety) of non-compound glume. If there are complex glumes in the wheat A / Huazhan field, they are miscellaneous plants and should be removed.

[0028] In the process of wheat A seed production, non-complex plants appear as hybrid plants and should be removed.

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Abstract

The invention provides a breeding method for a hybrid rice three-line male sterile line with a recessive marker. The recessive marker used by the method is a multi-awn marker and specifically comprises the steps that: 1) a material with the multi-awn marker is used to hybridize with a non-multi-awn material, a generation F1 is expressed to be non-multi-awn, the multi-awn plants and the non-multi-awn plants of a generation F2 are separated according to a ratio of 1:3, and fertile parents with the multi-awn markers are bred through multi-generation directional selection; and 2) the multi-awn fertile plants obtained in the step 1 are taken as male parents which hybridize with the non-multi-awn three-line male sterile line, sterile plants are selected from the generation F1, the sterile plants selected from the generation F1 continuously back-cross with the paternal multi-awn fertile plants, thoroughly abortive individual plants with the multi-awn marker are selected from generations after multi-generation back-crossing and finally the rice three-line male sterile line with the recessive marker is bred. The method provided by the invention has the advantages that the purity of rice seeds can be detected and abnormal plants in a paddy field can be identified intuitively, conveniently, rapidly and accurately.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for breeding a three-line male sterile line of hybrid rice with a recessive marker and its application. Background technique [0002] The level of seed purity is the primary index to measure the quality of the first-generation hybrid seeds, and is also an important basis for seed grading. The level of purity directly affects the yield and product quality of the first-generation hybrids. In the process of hybrid seed production, due to the influence of factors such as isolation conditions, parental purity, and parental variability, the purity of hybrids will decrease. Therefore, the rapid identification of the purity and purity of hybrid rice seeds has always attracted the attention of farmers and relevant departments. Currently commonly used identification methods include Hainan or local field planting identification, isoenzyme electrophoresis identifica...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
Inventor 徐小红李土明周卫营曲姗姗刘杰阴云伙彭炳生
Owner 江西现代种业股份有限公司
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